Molecular characterization of hepatitis A virus circulating in Uttar Pradesh, India: A hospital-based study

BACKGROUND & OBJECTIVES: Hepatitis A is prevalent worldwide and is among the leading cause of acute viral hepatitis in India. Major geographical differences in endemicity of hepatitis A are closely related to hygienic and sanitary conditions and other indicators of the level of socio-economic de...

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Autores principales: Prakash, Shantanu, Shukla, Suruchi, Shukla, Ratnam, Bhagat, Amit, Srivastava, Shakti S., Jain, Amita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7371058/
https://www.ncbi.nlm.nih.gov/pubmed/32461402
http://dx.doi.org/10.4103/ijmr.IJMR_429_18
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author Prakash, Shantanu
Shukla, Suruchi
Shukla, Ratnam
Bhagat, Amit
Srivastava, Shakti S.
Jain, Amita
author_facet Prakash, Shantanu
Shukla, Suruchi
Shukla, Ratnam
Bhagat, Amit
Srivastava, Shakti S.
Jain, Amita
author_sort Prakash, Shantanu
collection PubMed
description BACKGROUND & OBJECTIVES: Hepatitis A is prevalent worldwide and is among the leading cause of acute viral hepatitis in India. Major geographical differences in endemicity of hepatitis A are closely related to hygienic and sanitary conditions and other indicators of the level of socio-economic development. The present study was aimed to know the seropositivity prevalence and predominant circulating strain of HAV in a north India. METHODS: Patients with acute viral hepatitis were enrolled. Blood samples were collected over a period of one year from June 2016 to May 2017. Serum samples were tested for anti-immunoglobulin M (IgM) HAV antibodies. The seropositive samples were analyzed for HAV-RNA by real-time reverse transcription-polymerase chain reaction (RT-PCR). Samples detected on molecular assay were subjected to conventional semi-nested RT-PCR for VP1 gene. Further sequencing of amplified RT-PCR products was done, and data were analyzed. RESULTS: A total of 1615 patients were enrolled, and serum samples were collected and tested. The male:female ratio was 1.3:1 with a mean age of 24.31±17.02 yr (range 0-83 yr). Among these, 128 (7.93%) were positive for anti-HAV IgM antibodies; 41.63 per cent of seropositive patients were in their childhood or early adolescent age group. Of all seropositive samples, 59 (46.09%) were positive for HAV RNA. Genotyping sequencing of 10 representative strains was carried out, and the circulating genotype was found to be IIIA. The nucleotide sequences showed homology among the strains. INTERPRETATION & CONCLUSIONS: Our results showed that hepatitis A was a common disease in children with IIIA as a circulating genotype in this region. In approximately 50 per cent of cases, HAV RNA could be detected. Higher number of HAV IgM-seropositive cases was observed during monsoon period.
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spelling pubmed-73710582020-07-30 Molecular characterization of hepatitis A virus circulating in Uttar Pradesh, India: A hospital-based study Prakash, Shantanu Shukla, Suruchi Shukla, Ratnam Bhagat, Amit Srivastava, Shakti S. Jain, Amita Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Hepatitis A is prevalent worldwide and is among the leading cause of acute viral hepatitis in India. Major geographical differences in endemicity of hepatitis A are closely related to hygienic and sanitary conditions and other indicators of the level of socio-economic development. The present study was aimed to know the seropositivity prevalence and predominant circulating strain of HAV in a north India. METHODS: Patients with acute viral hepatitis were enrolled. Blood samples were collected over a period of one year from June 2016 to May 2017. Serum samples were tested for anti-immunoglobulin M (IgM) HAV antibodies. The seropositive samples were analyzed for HAV-RNA by real-time reverse transcription-polymerase chain reaction (RT-PCR). Samples detected on molecular assay were subjected to conventional semi-nested RT-PCR for VP1 gene. Further sequencing of amplified RT-PCR products was done, and data were analyzed. RESULTS: A total of 1615 patients were enrolled, and serum samples were collected and tested. The male:female ratio was 1.3:1 with a mean age of 24.31±17.02 yr (range 0-83 yr). Among these, 128 (7.93%) were positive for anti-HAV IgM antibodies; 41.63 per cent of seropositive patients were in their childhood or early adolescent age group. Of all seropositive samples, 59 (46.09%) were positive for HAV RNA. Genotyping sequencing of 10 representative strains was carried out, and the circulating genotype was found to be IIIA. The nucleotide sequences showed homology among the strains. INTERPRETATION & CONCLUSIONS: Our results showed that hepatitis A was a common disease in children with IIIA as a circulating genotype in this region. In approximately 50 per cent of cases, HAV RNA could be detected. Higher number of HAV IgM-seropositive cases was observed during monsoon period. Wolters Kluwer - Medknow 2020-04 /pmc/articles/PMC7371058/ /pubmed/32461402 http://dx.doi.org/10.4103/ijmr.IJMR_429_18 Text en Copyright: © 2020 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Prakash, Shantanu
Shukla, Suruchi
Shukla, Ratnam
Bhagat, Amit
Srivastava, Shakti S.
Jain, Amita
Molecular characterization of hepatitis A virus circulating in Uttar Pradesh, India: A hospital-based study
title Molecular characterization of hepatitis A virus circulating in Uttar Pradesh, India: A hospital-based study
title_full Molecular characterization of hepatitis A virus circulating in Uttar Pradesh, India: A hospital-based study
title_fullStr Molecular characterization of hepatitis A virus circulating in Uttar Pradesh, India: A hospital-based study
title_full_unstemmed Molecular characterization of hepatitis A virus circulating in Uttar Pradesh, India: A hospital-based study
title_short Molecular characterization of hepatitis A virus circulating in Uttar Pradesh, India: A hospital-based study
title_sort molecular characterization of hepatitis a virus circulating in uttar pradesh, india: a hospital-based study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7371058/
https://www.ncbi.nlm.nih.gov/pubmed/32461402
http://dx.doi.org/10.4103/ijmr.IJMR_429_18
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