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Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase

The role of non-bilayer lipids and non-lamellar lipid phases in biological membranes is an enigmatic problem of membrane biology. Non-bilayer lipids are present in large amounts in all membranes; in energy-converting membranes they constitute about half of their total lipid content—yet their functio...

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Autores principales: Dlouhý, Ondřej, Kurasová, Irena, Karlický, Václav, Javornik, Uroš, Šket, Primož, Petrova, Nia Z., Krumova, Sashka B., Plavec, Janez, Ughy, Bettina, Špunda, Vladimír, Garab, Győző
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7371714/
https://www.ncbi.nlm.nih.gov/pubmed/32686730
http://dx.doi.org/10.1038/s41598-020-68854-x
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author Dlouhý, Ondřej
Kurasová, Irena
Karlický, Václav
Javornik, Uroš
Šket, Primož
Petrova, Nia Z.
Krumova, Sashka B.
Plavec, Janez
Ughy, Bettina
Špunda, Vladimír
Garab, Győző
author_facet Dlouhý, Ondřej
Kurasová, Irena
Karlický, Václav
Javornik, Uroš
Šket, Primož
Petrova, Nia Z.
Krumova, Sashka B.
Plavec, Janez
Ughy, Bettina
Špunda, Vladimír
Garab, Győző
author_sort Dlouhý, Ondřej
collection PubMed
description The role of non-bilayer lipids and non-lamellar lipid phases in biological membranes is an enigmatic problem of membrane biology. Non-bilayer lipids are present in large amounts in all membranes; in energy-converting membranes they constitute about half of their total lipid content—yet their functional state is a bilayer. In vitro experiments revealed that the functioning of the water-soluble violaxanthin de-epoxidase (VDE) enzyme of plant thylakoids requires the presence of a non-bilayer lipid phase. (31)P-NMR spectroscopy has provided evidence on lipid polymorphism in functional thylakoid membranes. Here we reveal reversible pH- and temperature-dependent changes of the lipid-phase behaviour, particularly the flexibility of isotropic non-lamellar phases, of isolated spinach thylakoids. These reorganizations are accompanied by changes in the permeability and thermodynamic parameters of the membranes and appear to control the activity of VDE and the photoprotective mechanism of non-photochemical quenching of chlorophyll-a fluorescence. The data demonstrate, for the first time in native membranes, the modulation of the activity of a water-soluble enzyme by a non-bilayer lipid phase.
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spelling pubmed-73717142020-07-22 Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase Dlouhý, Ondřej Kurasová, Irena Karlický, Václav Javornik, Uroš Šket, Primož Petrova, Nia Z. Krumova, Sashka B. Plavec, Janez Ughy, Bettina Špunda, Vladimír Garab, Győző Sci Rep Article The role of non-bilayer lipids and non-lamellar lipid phases in biological membranes is an enigmatic problem of membrane biology. Non-bilayer lipids are present in large amounts in all membranes; in energy-converting membranes they constitute about half of their total lipid content—yet their functional state is a bilayer. In vitro experiments revealed that the functioning of the water-soluble violaxanthin de-epoxidase (VDE) enzyme of plant thylakoids requires the presence of a non-bilayer lipid phase. (31)P-NMR spectroscopy has provided evidence on lipid polymorphism in functional thylakoid membranes. Here we reveal reversible pH- and temperature-dependent changes of the lipid-phase behaviour, particularly the flexibility of isotropic non-lamellar phases, of isolated spinach thylakoids. These reorganizations are accompanied by changes in the permeability and thermodynamic parameters of the membranes and appear to control the activity of VDE and the photoprotective mechanism of non-photochemical quenching of chlorophyll-a fluorescence. The data demonstrate, for the first time in native membranes, the modulation of the activity of a water-soluble enzyme by a non-bilayer lipid phase. Nature Publishing Group UK 2020-07-20 /pmc/articles/PMC7371714/ /pubmed/32686730 http://dx.doi.org/10.1038/s41598-020-68854-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dlouhý, Ondřej
Kurasová, Irena
Karlický, Václav
Javornik, Uroš
Šket, Primož
Petrova, Nia Z.
Krumova, Sashka B.
Plavec, Janez
Ughy, Bettina
Špunda, Vladimír
Garab, Győző
Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase
title Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase
title_full Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase
title_fullStr Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase
title_full_unstemmed Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase
title_short Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase
title_sort modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7371714/
https://www.ncbi.nlm.nih.gov/pubmed/32686730
http://dx.doi.org/10.1038/s41598-020-68854-x
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