Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Single-chain variable fragments (scFvs) have gained increased attention among researchers in both academic and industrial fields owing to simple production in E. coli. The E. coli periplasm has been the site of choice for the expression of scFv molecules due to its oxidizing milieu facilitating corr...

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Detalles Bibliográficos
Autores principales: Ghamghami, Elham, Abri Aghdam, Marjan, Tohidkia, Mohammad Reza, Ahmadikhah, Asadollah, Khanmohammadi, Morteza, Mehdipour, Tayebeh, Mokhtarzadeh, Ahad, Baradaran, Behzad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7371774/
https://www.ncbi.nlm.nih.gov/pubmed/32691183
http://dx.doi.org/10.1186/s13568-020-01063-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7371774/
https://www.ncbi.nlm.nih.gov/pubmed/32691183
http://dx.doi.org/10.1186/s13568-020-01063-x

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