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Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed
Novel mutant alleles related to isoflavone content are useful for breeding programs to improve the disease resistance and nutritional content of soybean. However, identification of mutant alleles from high-density mutant libraries is expensive and time-consuming because soybean has a large, complica...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society of Breeding
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7372027/ https://www.ncbi.nlm.nih.gov/pubmed/32714048 http://dx.doi.org/10.1270/jsbbs.19134 |
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author | Sarkar, Md. Abdur Rauf Otsu, Wakana Suzuki, Akihiro Hashimoto, Fumio Anai, Toyoaki Watanabe, Satoshi |
author_facet | Sarkar, Md. Abdur Rauf Otsu, Wakana Suzuki, Akihiro Hashimoto, Fumio Anai, Toyoaki Watanabe, Satoshi |
author_sort | Sarkar, Md. Abdur Rauf |
collection | PubMed |
description | Novel mutant alleles related to isoflavone content are useful for breeding programs to improve the disease resistance and nutritional content of soybean. However, identification of mutant alleles from high-density mutant libraries is expensive and time-consuming because soybean has a large, complicated genome. Here, we identified the gene responsible for increased genistein-to-daidzein ratio in seed of the mutant line F333ES017D9. For this purpose, we used a time- and cost-effective approach based on selective genotyping of a small number of F(2) plants showing the mutant phenotype with nearest-neighboring-nucleotide substitution–high-resolution melting analysis markers, followed by alignment of short reads obtained by next-generation sequencing analysis with the identified locus. In the mutant line, GmCHR5 harbored a single-base deletion that caused a change in the substrate flow in the isoflavone biosynthetic pathway towards genistein. Mutated GmCHR5 was expressed at a lower level during seed development than wild-type GmCHR5. Ectopic overexpression of GmCHR5 increased the production of daidzein derivatives in both the wild-type and mutant plants. The present strategy will be useful for accelerating identification of mutant alleles responsible for traits of interest in agronomically important crops. |
format | Online Article Text |
id | pubmed-7372027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Japanese Society of Breeding |
record_format | MEDLINE/PubMed |
spelling | pubmed-73720272020-07-23 Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed Sarkar, Md. Abdur Rauf Otsu, Wakana Suzuki, Akihiro Hashimoto, Fumio Anai, Toyoaki Watanabe, Satoshi Breed Sci Research Paper Novel mutant alleles related to isoflavone content are useful for breeding programs to improve the disease resistance and nutritional content of soybean. However, identification of mutant alleles from high-density mutant libraries is expensive and time-consuming because soybean has a large, complicated genome. Here, we identified the gene responsible for increased genistein-to-daidzein ratio in seed of the mutant line F333ES017D9. For this purpose, we used a time- and cost-effective approach based on selective genotyping of a small number of F(2) plants showing the mutant phenotype with nearest-neighboring-nucleotide substitution–high-resolution melting analysis markers, followed by alignment of short reads obtained by next-generation sequencing analysis with the identified locus. In the mutant line, GmCHR5 harbored a single-base deletion that caused a change in the substrate flow in the isoflavone biosynthetic pathway towards genistein. Mutated GmCHR5 was expressed at a lower level during seed development than wild-type GmCHR5. Ectopic overexpression of GmCHR5 increased the production of daidzein derivatives in both the wild-type and mutant plants. The present strategy will be useful for accelerating identification of mutant alleles responsible for traits of interest in agronomically important crops. Japanese Society of Breeding 2020-06 2020-05-19 /pmc/articles/PMC7372027/ /pubmed/32714048 http://dx.doi.org/10.1270/jsbbs.19134 Text en Copyright © 2020 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Sarkar, Md. Abdur Rauf Otsu, Wakana Suzuki, Akihiro Hashimoto, Fumio Anai, Toyoaki Watanabe, Satoshi Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed |
title | Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed |
title_full | Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed |
title_fullStr | Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed |
title_full_unstemmed | Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed |
title_short | Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed |
title_sort | single-base deletion in gmchr5 increases the genistein-to-daidzein ratio in soybean seed |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7372027/ https://www.ncbi.nlm.nih.gov/pubmed/32714048 http://dx.doi.org/10.1270/jsbbs.19134 |
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