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Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market

Petunia plants with unusual orange flowers were noticed on the European market and confirmed to be genetically modified (GM) by the Finnish authorities in spring 2017. Later in 2017, inspections and controls performed by several official laboratories of national competent authorities in the European...

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Autores principales: Voorhuijzen, Marleen M., Prins, Theo W., Belter, Anke, Bendiek, Joachim, Brünen-Nieweler, Claudia, van Dijk, Jeroen P., Goerlich, Ottmar, Kok, Esther J., Pickel, Benjamin, Scholtens, Ingrid M. J., Stolz, Andrea, Grohmann, Lutz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7372090/
https://www.ncbi.nlm.nih.gov/pubmed/32760413
http://dx.doi.org/10.3389/fpls.2020.01047
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author Voorhuijzen, Marleen M.
Prins, Theo W.
Belter, Anke
Bendiek, Joachim
Brünen-Nieweler, Claudia
van Dijk, Jeroen P.
Goerlich, Ottmar
Kok, Esther J.
Pickel, Benjamin
Scholtens, Ingrid M. J.
Stolz, Andrea
Grohmann, Lutz
author_facet Voorhuijzen, Marleen M.
Prins, Theo W.
Belter, Anke
Bendiek, Joachim
Brünen-Nieweler, Claudia
van Dijk, Jeroen P.
Goerlich, Ottmar
Kok, Esther J.
Pickel, Benjamin
Scholtens, Ingrid M. J.
Stolz, Andrea
Grohmann, Lutz
author_sort Voorhuijzen, Marleen M.
collection PubMed
description Petunia plants with unusual orange flowers were noticed on the European market and confirmed to be genetically modified (GM) by the Finnish authorities in spring 2017. Later in 2017, inspections and controls performed by several official laboratories of national competent authorities in the European Union detected several GM petunia varieties with orange flowers, but also another group of unusually colored flowers. In the latter group, a so far undetected gene coding for a flavonoid 3’5’ hydroxylase (F3’5’H) responsible for the purple color was identified by German and Dutch authorities, suggesting that the petunias found on the markets contain different genetic constructs. Here, a strategy is described for the identification of GM petunia varieties. It is based on an initial GMO screening for known elements using (real-time) PCR and subsequent identification of the insertion sites by a gene walking-like approach called ALF (amplification of linearly-enriched fragments) in combination with Sanger and MinION sequencing. The results indicate that the positively identified GM petunias can be traced back to two dissimilar GM events used for breeding of the different varieties. The test results also confirm that the transgenic petunia event RL01-17 used in the first German field trial in 1991 is not the origin of the GM petunias sold on the market. On basis of the obtained sequence data, event-specific real-time PCR confirmatory methods were developed and validated. These methods are applicable for the rapid detection and identification of GM petunias in routine analysis. In addition, a decision support system was developed for revealing the most likely origin of the GM petunia.
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spelling pubmed-73720902020-08-04 Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market Voorhuijzen, Marleen M. Prins, Theo W. Belter, Anke Bendiek, Joachim Brünen-Nieweler, Claudia van Dijk, Jeroen P. Goerlich, Ottmar Kok, Esther J. Pickel, Benjamin Scholtens, Ingrid M. J. Stolz, Andrea Grohmann, Lutz Front Plant Sci Plant Science Petunia plants with unusual orange flowers were noticed on the European market and confirmed to be genetically modified (GM) by the Finnish authorities in spring 2017. Later in 2017, inspections and controls performed by several official laboratories of national competent authorities in the European Union detected several GM petunia varieties with orange flowers, but also another group of unusually colored flowers. In the latter group, a so far undetected gene coding for a flavonoid 3’5’ hydroxylase (F3’5’H) responsible for the purple color was identified by German and Dutch authorities, suggesting that the petunias found on the markets contain different genetic constructs. Here, a strategy is described for the identification of GM petunia varieties. It is based on an initial GMO screening for known elements using (real-time) PCR and subsequent identification of the insertion sites by a gene walking-like approach called ALF (amplification of linearly-enriched fragments) in combination with Sanger and MinION sequencing. The results indicate that the positively identified GM petunias can be traced back to two dissimilar GM events used for breeding of the different varieties. The test results also confirm that the transgenic petunia event RL01-17 used in the first German field trial in 1991 is not the origin of the GM petunias sold on the market. On basis of the obtained sequence data, event-specific real-time PCR confirmatory methods were developed and validated. These methods are applicable for the rapid detection and identification of GM petunias in routine analysis. In addition, a decision support system was developed for revealing the most likely origin of the GM petunia. Frontiers Media S.A. 2020-07-14 /pmc/articles/PMC7372090/ /pubmed/32760413 http://dx.doi.org/10.3389/fpls.2020.01047 Text en Copyright © 2020 Voorhuijzen, Prins, Belter, Bendiek, Brünen-Nieweler, van Dijk, Goerlich, Kok, Pickel, Scholtens, Stolz and Grohmann http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Voorhuijzen, Marleen M.
Prins, Theo W.
Belter, Anke
Bendiek, Joachim
Brünen-Nieweler, Claudia
van Dijk, Jeroen P.
Goerlich, Ottmar
Kok, Esther J.
Pickel, Benjamin
Scholtens, Ingrid M. J.
Stolz, Andrea
Grohmann, Lutz
Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market
title Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market
title_full Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market
title_fullStr Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market
title_full_unstemmed Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market
title_short Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market
title_sort molecular characterization and event-specific real-time pcr detection of two dissimilar groups of genetically modified petunia (petunia x hybrida) sold on the market
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7372090/
https://www.ncbi.nlm.nih.gov/pubmed/32760413
http://dx.doi.org/10.3389/fpls.2020.01047
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