Cargando…
A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis
BACKGROUND: Neisseria meningitidis is a major cause of bacterial meningitis, and these infections are associated with a high mortality rate. Rapid and reliable diagnosis of bacterial meningitis is critical in clinical practice. However, this disease often occurs in economically depressed areas, so a...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7372874/ https://www.ncbi.nlm.nih.gov/pubmed/32689953 http://dx.doi.org/10.1186/s12879-020-05250-w |
_version_ | 1783561400701943808 |
---|---|
author | Fan, Shu-Jin Tan, Hong-Kun Xu, Yu-Cheng Chen, Yuan-Zhi Xie, Tian-Ao Pan, Zhi-Yong Yang, Shi-Ou Li, Qin Li, Xiao-yan Li, Zhen-Xing Guo, Xu-Guang |
author_facet | Fan, Shu-Jin Tan, Hong-Kun Xu, Yu-Cheng Chen, Yuan-Zhi Xie, Tian-Ao Pan, Zhi-Yong Yang, Shi-Ou Li, Qin Li, Xiao-yan Li, Zhen-Xing Guo, Xu-Guang |
author_sort | Fan, Shu-Jin |
collection | PubMed |
description | BACKGROUND: Neisseria meningitidis is a major cause of bacterial meningitis, and these infections are associated with a high mortality rate. Rapid and reliable diagnosis of bacterial meningitis is critical in clinical practice. However, this disease often occurs in economically depressed areas, so an inexpensive, easy to use, and accurate technology is needed. We performed a pooled-analysis to assess the potential of the recently developed loop-mediated isothermal amplification (LAMP) assay for detection of meningococcus. METHODS: Pubmed, Embase, and Web of Science were searched to identify original studies that used the LAMP assay to detect meningococcus. After pooling of data, the sensitivity and specificity were calculated, a summary receiver operating characteristic (SROC) curve was determined, and the area under the SROC curve was computed to determine diagnostic accuracy. Publication bias was assessed using Deek’s funnel plot. RESULTS: We examined 14 studies within 6 publications. The LAMP assay had high sensitivity (94%) and specificity (100%) in the detection of meningococcus in all studies. The area under the SROC curve (0.980) indicated high overall accuracy of the LAMP assay. There was no evidence of publication bias. DISCUSSION: The LAMP assay has accuracy comparable to bacterial culture and PCR for detection of meningococcus, but is less expensive and easier to use. We suggest the adoption of the LAMP assay to detect meningococcus, especially in economically depressed areas. |
format | Online Article Text |
id | pubmed-7372874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-73728742020-07-21 A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis Fan, Shu-Jin Tan, Hong-Kun Xu, Yu-Cheng Chen, Yuan-Zhi Xie, Tian-Ao Pan, Zhi-Yong Yang, Shi-Ou Li, Qin Li, Xiao-yan Li, Zhen-Xing Guo, Xu-Guang BMC Infect Dis Research Article BACKGROUND: Neisseria meningitidis is a major cause of bacterial meningitis, and these infections are associated with a high mortality rate. Rapid and reliable diagnosis of bacterial meningitis is critical in clinical practice. However, this disease often occurs in economically depressed areas, so an inexpensive, easy to use, and accurate technology is needed. We performed a pooled-analysis to assess the potential of the recently developed loop-mediated isothermal amplification (LAMP) assay for detection of meningococcus. METHODS: Pubmed, Embase, and Web of Science were searched to identify original studies that used the LAMP assay to detect meningococcus. After pooling of data, the sensitivity and specificity were calculated, a summary receiver operating characteristic (SROC) curve was determined, and the area under the SROC curve was computed to determine diagnostic accuracy. Publication bias was assessed using Deek’s funnel plot. RESULTS: We examined 14 studies within 6 publications. The LAMP assay had high sensitivity (94%) and specificity (100%) in the detection of meningococcus in all studies. The area under the SROC curve (0.980) indicated high overall accuracy of the LAMP assay. There was no evidence of publication bias. DISCUSSION: The LAMP assay has accuracy comparable to bacterial culture and PCR for detection of meningococcus, but is less expensive and easier to use. We suggest the adoption of the LAMP assay to detect meningococcus, especially in economically depressed areas. BioMed Central 2020-07-20 /pmc/articles/PMC7372874/ /pubmed/32689953 http://dx.doi.org/10.1186/s12879-020-05250-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Fan, Shu-Jin Tan, Hong-Kun Xu, Yu-Cheng Chen, Yuan-Zhi Xie, Tian-Ao Pan, Zhi-Yong Yang, Shi-Ou Li, Qin Li, Xiao-yan Li, Zhen-Xing Guo, Xu-Guang A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis |
title | A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis |
title_full | A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis |
title_fullStr | A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis |
title_full_unstemmed | A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis |
title_short | A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis |
title_sort | pooled analysis of the lamp assay for the detection of neisseria meningitidis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7372874/ https://www.ncbi.nlm.nih.gov/pubmed/32689953 http://dx.doi.org/10.1186/s12879-020-05250-w |
work_keys_str_mv | AT fanshujin apooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT tanhongkun apooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT xuyucheng apooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT chenyuanzhi apooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT xietianao apooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT panzhiyong apooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT yangshiou apooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT liqin apooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT lixiaoyan apooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT lizhenxing apooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT guoxuguang apooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT fanshujin pooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT tanhongkun pooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT xuyucheng pooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT chenyuanzhi pooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT xietianao pooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT panzhiyong pooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT yangshiou pooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT liqin pooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT lixiaoyan pooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT lizhenxing pooledanalysisofthelampassayforthedetectionofneisseriameningitidis AT guoxuguang pooledanalysisofthelampassayforthedetectionofneisseriameningitidis |