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Differential immune activation profile of SARS-CoV-2 and SARS-CoV infection in human lung and intestinal cells: Implications for treatment with IFN-β and IFN inducer

OBJECTIVES: Respiratory and intestinal tract are two primary target organs of SARS-CoV-2 infection. However, detailed characterization of the host-virus interplay in infected human lung and intestinal epithelial cells is lacking. METHODS: We utilized immunofluorescence assays, flow cytometry, and RT...

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Autores principales: Shuai, Huiping, Chu, Hin, Hou, Yuxin, Yang, Dong, Wang, Yixin, Hu, Bingjie, Huang, Xiner, Zhang, Xi, Chai, Yue, Cai, Jian-Piao, Chan, Jasper Fuk-Woo, Yuen, Kwok-Yung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The British Infection Association. Published by Elsevier Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373021/
https://www.ncbi.nlm.nih.gov/pubmed/32707230
http://dx.doi.org/10.1016/j.jinf.2020.07.016
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author Shuai, Huiping
Chu, Hin
Hou, Yuxin
Yang, Dong
Wang, Yixin
Hu, Bingjie
Huang, Xiner
Zhang, Xi
Chai, Yue
Cai, Jian-Piao
Chan, Jasper Fuk-Woo
Yuen, Kwok-Yung
author_facet Shuai, Huiping
Chu, Hin
Hou, Yuxin
Yang, Dong
Wang, Yixin
Hu, Bingjie
Huang, Xiner
Zhang, Xi
Chai, Yue
Cai, Jian-Piao
Chan, Jasper Fuk-Woo
Yuen, Kwok-Yung
author_sort Shuai, Huiping
collection PubMed
description OBJECTIVES: Respiratory and intestinal tract are two primary target organs of SARS-CoV-2 infection. However, detailed characterization of the host-virus interplay in infected human lung and intestinal epithelial cells is lacking. METHODS: We utilized immunofluorescence assays, flow cytometry, and RT-qPCR to delineate the virological features and the innate immune response of the host cells against SARS-CoV-2 infection in two prototype human cell lines representing the human lung (Calu3) and intestinal (Caco2) epithelium when compared with SARS-CoV. RESULTS: Lung epithelial cells were significantly more susceptible to SARS-CoV-2 compared to SARS-CoV. However, SARS-CoV-2 infection induced an attenuated pro-inflammatory cytokines/chemokines induction and type I and type II IFN responses. A single dose of 10 U/mL interferon-β (IFNβ) pretreatment potently protected both Calu3 and Caco2 against SARS-CoV-2 infection. Interestingly, SARS-CoV-2 was more sensitive to the pretreatment with IFNβ and IFN inducer than SARS-CoV in Calu3. CONCLUSIONS: Despite robust infection in both human lung and intestinal epithelial cells, SARS-CoV-2 could attenuate the virus-induced pro-inflammatory response and IFN response. Pre-activation of the type I IFN signaling pathway primed a highly efficient antiviral response in the host against SARS-CoV-2 infection, which could serve as a potential therapeutic and prophylactic maneuver to COVID-19 patients.
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spelling pubmed-73730212020-07-22 Differential immune activation profile of SARS-CoV-2 and SARS-CoV infection in human lung and intestinal cells: Implications for treatment with IFN-β and IFN inducer Shuai, Huiping Chu, Hin Hou, Yuxin Yang, Dong Wang, Yixin Hu, Bingjie Huang, Xiner Zhang, Xi Chai, Yue Cai, Jian-Piao Chan, Jasper Fuk-Woo Yuen, Kwok-Yung J Infect Full Length Article OBJECTIVES: Respiratory and intestinal tract are two primary target organs of SARS-CoV-2 infection. However, detailed characterization of the host-virus interplay in infected human lung and intestinal epithelial cells is lacking. METHODS: We utilized immunofluorescence assays, flow cytometry, and RT-qPCR to delineate the virological features and the innate immune response of the host cells against SARS-CoV-2 infection in two prototype human cell lines representing the human lung (Calu3) and intestinal (Caco2) epithelium when compared with SARS-CoV. RESULTS: Lung epithelial cells were significantly more susceptible to SARS-CoV-2 compared to SARS-CoV. However, SARS-CoV-2 infection induced an attenuated pro-inflammatory cytokines/chemokines induction and type I and type II IFN responses. A single dose of 10 U/mL interferon-β (IFNβ) pretreatment potently protected both Calu3 and Caco2 against SARS-CoV-2 infection. Interestingly, SARS-CoV-2 was more sensitive to the pretreatment with IFNβ and IFN inducer than SARS-CoV in Calu3. CONCLUSIONS: Despite robust infection in both human lung and intestinal epithelial cells, SARS-CoV-2 could attenuate the virus-induced pro-inflammatory response and IFN response. Pre-activation of the type I IFN signaling pathway primed a highly efficient antiviral response in the host against SARS-CoV-2 infection, which could serve as a potential therapeutic and prophylactic maneuver to COVID-19 patients. The British Infection Association. Published by Elsevier Ltd. 2020-10 2020-07-21 /pmc/articles/PMC7373021/ /pubmed/32707230 http://dx.doi.org/10.1016/j.jinf.2020.07.016 Text en © 2020 The British Infection Association. Published by Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Full Length Article
Shuai, Huiping
Chu, Hin
Hou, Yuxin
Yang, Dong
Wang, Yixin
Hu, Bingjie
Huang, Xiner
Zhang, Xi
Chai, Yue
Cai, Jian-Piao
Chan, Jasper Fuk-Woo
Yuen, Kwok-Yung
Differential immune activation profile of SARS-CoV-2 and SARS-CoV infection in human lung and intestinal cells: Implications for treatment with IFN-β and IFN inducer
title Differential immune activation profile of SARS-CoV-2 and SARS-CoV infection in human lung and intestinal cells: Implications for treatment with IFN-β and IFN inducer
title_full Differential immune activation profile of SARS-CoV-2 and SARS-CoV infection in human lung and intestinal cells: Implications for treatment with IFN-β and IFN inducer
title_fullStr Differential immune activation profile of SARS-CoV-2 and SARS-CoV infection in human lung and intestinal cells: Implications for treatment with IFN-β and IFN inducer
title_full_unstemmed Differential immune activation profile of SARS-CoV-2 and SARS-CoV infection in human lung and intestinal cells: Implications for treatment with IFN-β and IFN inducer
title_short Differential immune activation profile of SARS-CoV-2 and SARS-CoV infection in human lung and intestinal cells: Implications for treatment with IFN-β and IFN inducer
title_sort differential immune activation profile of sars-cov-2 and sars-cov infection in human lung and intestinal cells: implications for treatment with ifn-β and ifn inducer
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373021/
https://www.ncbi.nlm.nih.gov/pubmed/32707230
http://dx.doi.org/10.1016/j.jinf.2020.07.016
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