Cargando…

Murine hepatoma treatment with mature dendritic cells stimulated by Trichinella spiralis excretory/secretory products

Excretory/Secretory Products (ESPs) of the nematode Trichinella spiralis contain antitumor-active substances that inhibit tumor growth. Mature dendritic cells (DCs) play a critical role in the antitumor immunity of the organism. As pathogen-derived products, it ought to be discussed whether T. spira...

Descripción completa

Detalles Bibliográficos
Autores principales: Ding, Jing, Liu, Xiaolei, Tang, Bin, Bai, Xue, Wang, Yang, Li, Shicun, Li, Jian, Liu, Mingyuan, Wang, Xuelin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373160/
https://www.ncbi.nlm.nih.gov/pubmed/32692308
http://dx.doi.org/10.1051/parasite/2020045
_version_ 1783561451834703872
author Ding, Jing
Liu, Xiaolei
Tang, Bin
Bai, Xue
Wang, Yang
Li, Shicun
Li, Jian
Liu, Mingyuan
Wang, Xuelin
author_facet Ding, Jing
Liu, Xiaolei
Tang, Bin
Bai, Xue
Wang, Yang
Li, Shicun
Li, Jian
Liu, Mingyuan
Wang, Xuelin
author_sort Ding, Jing
collection PubMed
description Excretory/Secretory Products (ESPs) of the nematode Trichinella spiralis contain antitumor-active substances that inhibit tumor growth. Mature dendritic cells (DCs) play a critical role in the antitumor immunity of the organism. As pathogen-derived products, it ought to be discussed whether T. spiralis ESPs will reduce the antitumor effect of mature DCs from the host before it is applied to patients’ tumors. Therefore, the aim of this work was to evaluate the immunological effect of DCs stimulated by T. spiralis ESPs in H22 tumor-bearing mice. H22 tumor model mice in this study were randomly divided into four groups according to the treatment: PBS control group, ESP group, DCs group, and DCs stimulated with T. spiralis ESP (ESP+DCs group). The antitumor effect was evaluated by tumor inhibition rate and cytokine detection using ELISA. The results showed significant inhibition in tumor growth in the ESP+DCs, DCs and ESP groups when compared with the PBS control group (p < 0.01, p < 0.01, and p < 0.05, respectively). However, no significant difference was observed on tumor inhibition rates between the ESP+DCs and DCs groups. The decrease in IL-4, IL-6, and IL-10, and the increase in IFN-γ between the DCs and ESP+DCs groups were also not significant. Therefore, DCs stimulated by ESP did not reduce the antitumor effect of mature DCs, which demonstrated that the T. spiralis ESP would not affect the antitumor effect of mature DCs by modulating the immune response of the host, and that ESPs are safe in antitumor immunology when applied in a tumor model mice.
format Online
Article
Text
id pubmed-7373160
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher EDP Sciences
record_format MEDLINE/PubMed
spelling pubmed-73731602020-07-30 Murine hepatoma treatment with mature dendritic cells stimulated by Trichinella spiralis excretory/secretory products Ding, Jing Liu, Xiaolei Tang, Bin Bai, Xue Wang, Yang Li, Shicun Li, Jian Liu, Mingyuan Wang, Xuelin Parasite Research Article Excretory/Secretory Products (ESPs) of the nematode Trichinella spiralis contain antitumor-active substances that inhibit tumor growth. Mature dendritic cells (DCs) play a critical role in the antitumor immunity of the organism. As pathogen-derived products, it ought to be discussed whether T. spiralis ESPs will reduce the antitumor effect of mature DCs from the host before it is applied to patients’ tumors. Therefore, the aim of this work was to evaluate the immunological effect of DCs stimulated by T. spiralis ESPs in H22 tumor-bearing mice. H22 tumor model mice in this study were randomly divided into four groups according to the treatment: PBS control group, ESP group, DCs group, and DCs stimulated with T. spiralis ESP (ESP+DCs group). The antitumor effect was evaluated by tumor inhibition rate and cytokine detection using ELISA. The results showed significant inhibition in tumor growth in the ESP+DCs, DCs and ESP groups when compared with the PBS control group (p < 0.01, p < 0.01, and p < 0.05, respectively). However, no significant difference was observed on tumor inhibition rates between the ESP+DCs and DCs groups. The decrease in IL-4, IL-6, and IL-10, and the increase in IFN-γ between the DCs and ESP+DCs groups were also not significant. Therefore, DCs stimulated by ESP did not reduce the antitumor effect of mature DCs, which demonstrated that the T. spiralis ESP would not affect the antitumor effect of mature DCs by modulating the immune response of the host, and that ESPs are safe in antitumor immunology when applied in a tumor model mice. EDP Sciences 2020-07-21 /pmc/articles/PMC7373160/ /pubmed/32692308 http://dx.doi.org/10.1051/parasite/2020045 Text en © J. Ding et al., published by EDP Sciences, 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ding, Jing
Liu, Xiaolei
Tang, Bin
Bai, Xue
Wang, Yang
Li, Shicun
Li, Jian
Liu, Mingyuan
Wang, Xuelin
Murine hepatoma treatment with mature dendritic cells stimulated by Trichinella spiralis excretory/secretory products
title Murine hepatoma treatment with mature dendritic cells stimulated by Trichinella spiralis excretory/secretory products
title_full Murine hepatoma treatment with mature dendritic cells stimulated by Trichinella spiralis excretory/secretory products
title_fullStr Murine hepatoma treatment with mature dendritic cells stimulated by Trichinella spiralis excretory/secretory products
title_full_unstemmed Murine hepatoma treatment with mature dendritic cells stimulated by Trichinella spiralis excretory/secretory products
title_short Murine hepatoma treatment with mature dendritic cells stimulated by Trichinella spiralis excretory/secretory products
title_sort murine hepatoma treatment with mature dendritic cells stimulated by trichinella spiralis excretory/secretory products
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373160/
https://www.ncbi.nlm.nih.gov/pubmed/32692308
http://dx.doi.org/10.1051/parasite/2020045
work_keys_str_mv AT dingjing murinehepatomatreatmentwithmaturedendriticcellsstimulatedbytrichinellaspiralisexcretorysecretoryproducts
AT liuxiaolei murinehepatomatreatmentwithmaturedendriticcellsstimulatedbytrichinellaspiralisexcretorysecretoryproducts
AT tangbin murinehepatomatreatmentwithmaturedendriticcellsstimulatedbytrichinellaspiralisexcretorysecretoryproducts
AT baixue murinehepatomatreatmentwithmaturedendriticcellsstimulatedbytrichinellaspiralisexcretorysecretoryproducts
AT wangyang murinehepatomatreatmentwithmaturedendriticcellsstimulatedbytrichinellaspiralisexcretorysecretoryproducts
AT lishicun murinehepatomatreatmentwithmaturedendriticcellsstimulatedbytrichinellaspiralisexcretorysecretoryproducts
AT lijian murinehepatomatreatmentwithmaturedendriticcellsstimulatedbytrichinellaspiralisexcretorysecretoryproducts
AT liumingyuan murinehepatomatreatmentwithmaturedendriticcellsstimulatedbytrichinellaspiralisexcretorysecretoryproducts
AT wangxuelin murinehepatomatreatmentwithmaturedendriticcellsstimulatedbytrichinellaspiralisexcretorysecretoryproducts