Critical Relevance of Stochastic Effects on Low-Bacterial-Biomass 16S rRNA Gene Analysis

The bacterial microbiome of human body sites, previously considered sterile, remains highly controversial because it can be challenging to isolate signal from noise when low-biomass samples are being analyzed. We tested the hypothesis that stochastic sequencing noise, separable from reagent contamin...

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Detalles Bibliográficos
Autores principales: Erb-Downward, John R., Falkowski, Nicole R., D’Souza, Jennifer C., McCloskey, Lisa M., McDonald, Roderick A., Brown, Christopher A., Shedden, Kerby, Dickson, Robert P., Freeman, Christine M., Stringer, Kathleen A., Foxman, Betsy, Huffnagle, Gary B., Curtis, Jeffrey L., Adar, Sara D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373192/
https://www.ncbi.nlm.nih.gov/pubmed/32518181
http://dx.doi.org/10.1128/mBio.00258-20

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