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Kinetics of Phenol Biodegradation by Heavy Metal Tolerant Rhizobacteria Glutamicibacter nicotianae MSSRFPD35 From Distillery Effluent Contaminated Soils
Biodegradation of phenol using bacteria is recognized as an efficient, environmentally friendly and cost-effective approach for reducing phenol pollutants compared to the current conventional physicochemical processes adopted. A potential phenol degrading bacterial strain Glutamicibacter nicotianae...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373764/ https://www.ncbi.nlm.nih.gov/pubmed/32760369 http://dx.doi.org/10.3389/fmicb.2020.01573 |
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author | Duraisamy, Purushothaman Sekar, Jegan Arunkumar, Anu D. Ramalingam, Prabavathy V. |
author_facet | Duraisamy, Purushothaman Sekar, Jegan Arunkumar, Anu D. Ramalingam, Prabavathy V. |
author_sort | Duraisamy, Purushothaman |
collection | PubMed |
description | Biodegradation of phenol using bacteria is recognized as an efficient, environmentally friendly and cost-effective approach for reducing phenol pollutants compared to the current conventional physicochemical processes adopted. A potential phenol degrading bacterial strain Glutamicibacter nicotianae MSSRFPD35 was isolated and identified from Canna indica rhizosphere grown in distillery effluent contaminated sites. It showed high phenol degrading efficiency up to 1117 mg L(–1) within 60 h by the secretion of catechol 1,2-dioxygenase via ortho intradial pathway. The strain MSSRFPD35 possess both the catechol 1,2 dioxygenase and catechol 2,3 dioxygenase coding genes that drive the ortho and meta pathways, but the enzymatic assay revealed that the strain cleaves catechol via ortho pathway. Haldane’s kinetic method was well fit to exponential growth data and the following kinetic parameter was obtained: μ(∗) = 0.574 h(–1), K(i) = 268.1, K(s) = 20.29 mg L(–1). The true μ(max) and S(m) were calculated as 0.37 h(–1) and 73.76 mg L(–1), respectively. The Haldane’s constant values were similar to earlier studies and healthy fitness depicted in correlation coefficient value R(2) of 0.98. Phenol degrading kinetic’s was predicted using Haldane’s model as q(max) 0.983, K(i)′ 517.5 and K(s)′ 9.152. Further, MSSRFPD35 was capable of utilizing different monocyclic and polycyclic aromatic hydrocarbons and to degrade phenol in the presence of different heavy metals. This study for the first time reports high phenol degrading efficiency of G. nicotianae MSSRFPD35 in the presence of toxic heavy metals. Thus, the strain G. nicotianae MSSRFPD35 can be exploited for the bioremediation of phenol and its derivatives polluted environments, co-contaminated with heavy metals. |
format | Online Article Text |
id | pubmed-7373764 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73737642020-08-04 Kinetics of Phenol Biodegradation by Heavy Metal Tolerant Rhizobacteria Glutamicibacter nicotianae MSSRFPD35 From Distillery Effluent Contaminated Soils Duraisamy, Purushothaman Sekar, Jegan Arunkumar, Anu D. Ramalingam, Prabavathy V. Front Microbiol Microbiology Biodegradation of phenol using bacteria is recognized as an efficient, environmentally friendly and cost-effective approach for reducing phenol pollutants compared to the current conventional physicochemical processes adopted. A potential phenol degrading bacterial strain Glutamicibacter nicotianae MSSRFPD35 was isolated and identified from Canna indica rhizosphere grown in distillery effluent contaminated sites. It showed high phenol degrading efficiency up to 1117 mg L(–1) within 60 h by the secretion of catechol 1,2-dioxygenase via ortho intradial pathway. The strain MSSRFPD35 possess both the catechol 1,2 dioxygenase and catechol 2,3 dioxygenase coding genes that drive the ortho and meta pathways, but the enzymatic assay revealed that the strain cleaves catechol via ortho pathway. Haldane’s kinetic method was well fit to exponential growth data and the following kinetic parameter was obtained: μ(∗) = 0.574 h(–1), K(i) = 268.1, K(s) = 20.29 mg L(–1). The true μ(max) and S(m) were calculated as 0.37 h(–1) and 73.76 mg L(–1), respectively. The Haldane’s constant values were similar to earlier studies and healthy fitness depicted in correlation coefficient value R(2) of 0.98. Phenol degrading kinetic’s was predicted using Haldane’s model as q(max) 0.983, K(i)′ 517.5 and K(s)′ 9.152. Further, MSSRFPD35 was capable of utilizing different monocyclic and polycyclic aromatic hydrocarbons and to degrade phenol in the presence of different heavy metals. This study for the first time reports high phenol degrading efficiency of G. nicotianae MSSRFPD35 in the presence of toxic heavy metals. Thus, the strain G. nicotianae MSSRFPD35 can be exploited for the bioremediation of phenol and its derivatives polluted environments, co-contaminated with heavy metals. Frontiers Media S.A. 2020-07-15 /pmc/articles/PMC7373764/ /pubmed/32760369 http://dx.doi.org/10.3389/fmicb.2020.01573 Text en Copyright © 2020 Duraisamy, Sekar, Arunkumar and Ramalingam. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Duraisamy, Purushothaman Sekar, Jegan Arunkumar, Anu D. Ramalingam, Prabavathy V. Kinetics of Phenol Biodegradation by Heavy Metal Tolerant Rhizobacteria Glutamicibacter nicotianae MSSRFPD35 From Distillery Effluent Contaminated Soils |
title | Kinetics of Phenol Biodegradation by Heavy Metal Tolerant Rhizobacteria Glutamicibacter nicotianae MSSRFPD35 From Distillery Effluent Contaminated Soils |
title_full | Kinetics of Phenol Biodegradation by Heavy Metal Tolerant Rhizobacteria Glutamicibacter nicotianae MSSRFPD35 From Distillery Effluent Contaminated Soils |
title_fullStr | Kinetics of Phenol Biodegradation by Heavy Metal Tolerant Rhizobacteria Glutamicibacter nicotianae MSSRFPD35 From Distillery Effluent Contaminated Soils |
title_full_unstemmed | Kinetics of Phenol Biodegradation by Heavy Metal Tolerant Rhizobacteria Glutamicibacter nicotianae MSSRFPD35 From Distillery Effluent Contaminated Soils |
title_short | Kinetics of Phenol Biodegradation by Heavy Metal Tolerant Rhizobacteria Glutamicibacter nicotianae MSSRFPD35 From Distillery Effluent Contaminated Soils |
title_sort | kinetics of phenol biodegradation by heavy metal tolerant rhizobacteria glutamicibacter nicotianae mssrfpd35 from distillery effluent contaminated soils |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373764/ https://www.ncbi.nlm.nih.gov/pubmed/32760369 http://dx.doi.org/10.3389/fmicb.2020.01573 |
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