Cargando…

Improving the Therapeutic Effect of Ultrasound Combined With Microbubbles on Muscular Tumor Xenografts With Appropriate Acoustic Pressure

Ultrasound combined with microbubbles (USMB) is a promising antitumor therapy because of its capability to selectively disrupt tumor perfusion. However, the antitumor effects of repeated USMB treatments have yet to be clarified. In this study, we established a VX2 muscular tumor xenograft model in r...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Yan, Yu, Meiling, Wang, Jie, Xi, Fen, Zhong, Jiali, Yang, Yuwen, Jin, Hai, Liu, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373785/
https://www.ncbi.nlm.nih.gov/pubmed/32760276
http://dx.doi.org/10.3389/fphar.2020.01057
_version_ 1783561561116246016
author He, Yan
Yu, Meiling
Wang, Jie
Xi, Fen
Zhong, Jiali
Yang, Yuwen
Jin, Hai
Liu, Jianhua
author_facet He, Yan
Yu, Meiling
Wang, Jie
Xi, Fen
Zhong, Jiali
Yang, Yuwen
Jin, Hai
Liu, Jianhua
author_sort He, Yan
collection PubMed
description Ultrasound combined with microbubbles (USMB) is a promising antitumor therapy because of its capability to selectively disrupt tumor perfusion. However, the antitumor effects of repeated USMB treatments have yet to be clarified. In this study, we established a VX2 muscular tumor xenograft model in rabbits, and performed USMB treatments at five different peak negative acoustic pressure levels (1.0, 2.0, 3.0, 4.0, or 5.0 MPa) to determine the appropriate acoustic pressure. To investigate whether repeated USMB treatments could improve the antitumor effects, a group of tumor-bearing rabbits was subjected to one USMB treatment per day for three consecutive days for comparison with the single-treatment group. Contrast-enhanced ultrasonic imaging and histological analyses showed that at an acoustic pressure of 4.0 MPa, USMB treatment contributed to substantial cessation of tumor perfusion, resulting in severe damage to the tumor cells and microvessels without causing significant effects on the normal tissue. Further, the percentages of damaged area and apoptotic cells in the tumor were significantly higher, and the tumor growth inhibition effect was more obvious in the multiple-treatment group than in the single USMB treatment group. These findings indicate that with an appropriate acoustic pressure, the USMB treatment can selectively destroy tumor vessels in muscular tumor xenograft models. Moreover, the repeated treatments strategy can significantly improve the antitumor effect. Therefore, our results provide a foundation for the clinical application of USMB to treat solid tumors using a novel therapeutic strategy.
format Online
Article
Text
id pubmed-7373785
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-73737852020-08-04 Improving the Therapeutic Effect of Ultrasound Combined With Microbubbles on Muscular Tumor Xenografts With Appropriate Acoustic Pressure He, Yan Yu, Meiling Wang, Jie Xi, Fen Zhong, Jiali Yang, Yuwen Jin, Hai Liu, Jianhua Front Pharmacol Pharmacology Ultrasound combined with microbubbles (USMB) is a promising antitumor therapy because of its capability to selectively disrupt tumor perfusion. However, the antitumor effects of repeated USMB treatments have yet to be clarified. In this study, we established a VX2 muscular tumor xenograft model in rabbits, and performed USMB treatments at five different peak negative acoustic pressure levels (1.0, 2.0, 3.0, 4.0, or 5.0 MPa) to determine the appropriate acoustic pressure. To investigate whether repeated USMB treatments could improve the antitumor effects, a group of tumor-bearing rabbits was subjected to one USMB treatment per day for three consecutive days for comparison with the single-treatment group. Contrast-enhanced ultrasonic imaging and histological analyses showed that at an acoustic pressure of 4.0 MPa, USMB treatment contributed to substantial cessation of tumor perfusion, resulting in severe damage to the tumor cells and microvessels without causing significant effects on the normal tissue. Further, the percentages of damaged area and apoptotic cells in the tumor were significantly higher, and the tumor growth inhibition effect was more obvious in the multiple-treatment group than in the single USMB treatment group. These findings indicate that with an appropriate acoustic pressure, the USMB treatment can selectively destroy tumor vessels in muscular tumor xenograft models. Moreover, the repeated treatments strategy can significantly improve the antitumor effect. Therefore, our results provide a foundation for the clinical application of USMB to treat solid tumors using a novel therapeutic strategy. Frontiers Media S.A. 2020-07-15 /pmc/articles/PMC7373785/ /pubmed/32760276 http://dx.doi.org/10.3389/fphar.2020.01057 Text en Copyright © 2020 He, Yu, Wang, Xi, Zhong, Yang, Jin and Liu http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
He, Yan
Yu, Meiling
Wang, Jie
Xi, Fen
Zhong, Jiali
Yang, Yuwen
Jin, Hai
Liu, Jianhua
Improving the Therapeutic Effect of Ultrasound Combined With Microbubbles on Muscular Tumor Xenografts With Appropriate Acoustic Pressure
title Improving the Therapeutic Effect of Ultrasound Combined With Microbubbles on Muscular Tumor Xenografts With Appropriate Acoustic Pressure
title_full Improving the Therapeutic Effect of Ultrasound Combined With Microbubbles on Muscular Tumor Xenografts With Appropriate Acoustic Pressure
title_fullStr Improving the Therapeutic Effect of Ultrasound Combined With Microbubbles on Muscular Tumor Xenografts With Appropriate Acoustic Pressure
title_full_unstemmed Improving the Therapeutic Effect of Ultrasound Combined With Microbubbles on Muscular Tumor Xenografts With Appropriate Acoustic Pressure
title_short Improving the Therapeutic Effect of Ultrasound Combined With Microbubbles on Muscular Tumor Xenografts With Appropriate Acoustic Pressure
title_sort improving the therapeutic effect of ultrasound combined with microbubbles on muscular tumor xenografts with appropriate acoustic pressure
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373785/
https://www.ncbi.nlm.nih.gov/pubmed/32760276
http://dx.doi.org/10.3389/fphar.2020.01057
work_keys_str_mv AT heyan improvingthetherapeuticeffectofultrasoundcombinedwithmicrobubblesonmusculartumorxenograftswithappropriateacousticpressure
AT yumeiling improvingthetherapeuticeffectofultrasoundcombinedwithmicrobubblesonmusculartumorxenograftswithappropriateacousticpressure
AT wangjie improvingthetherapeuticeffectofultrasoundcombinedwithmicrobubblesonmusculartumorxenograftswithappropriateacousticpressure
AT xifen improvingthetherapeuticeffectofultrasoundcombinedwithmicrobubblesonmusculartumorxenograftswithappropriateacousticpressure
AT zhongjiali improvingthetherapeuticeffectofultrasoundcombinedwithmicrobubblesonmusculartumorxenograftswithappropriateacousticpressure
AT yangyuwen improvingthetherapeuticeffectofultrasoundcombinedwithmicrobubblesonmusculartumorxenograftswithappropriateacousticpressure
AT jinhai improvingthetherapeuticeffectofultrasoundcombinedwithmicrobubblesonmusculartumorxenograftswithappropriateacousticpressure
AT liujianhua improvingthetherapeuticeffectofultrasoundcombinedwithmicrobubblesonmusculartumorxenograftswithappropriateacousticpressure