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Improved bacterial RNA-seq by Cas9-based depletion of ribosomal RNA reads

A major challenge for RNA-seq analysis of gene expression is to achieve sufficient coverage of informative nonribosomal transcripts. In eukaryotic samples, this is typically achieved by selective oligo(dT)-priming of messenger RNAs to exclude ribosomal RNA (rRNA) during cDNA synthesis. However, this...

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Detalles Bibliográficos
Autores principales:	Prezza, Gianluca, Heckel, Tobias, Dietrich, Sascha, Homberger, Christina, Westermann, Alexander J., Vogel, Jörg
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2020
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373992/ https://www.ncbi.nlm.nih.gov/pubmed/32345633 http://dx.doi.org/10.1261/rna.075945.120

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