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Serosurvey and molecular detection of the main zoonotic parasites carried by commensal Rattus norvegicus population in Tehran, Iran
BACKGROUND: Rattus norvegicus are reservoirs for transmission of various zoonotic parasites, and they have become a threat to public health worldwide. Given the large number and the significant presence of R. norvegicus throughout the city of Tehran, this study aims to assess the frequency of zoonot...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374959/ https://www.ncbi.nlm.nih.gov/pubmed/32704233 http://dx.doi.org/10.1186/s41182-020-00246-3 |
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author | Azimi, Taher Pourmand, Mohammad Reza Fallah, Fatemeh Karimi, Abdollah Mansour-Ghanaie, Roxana Hoseini-Alfatemi, Seyedeh Mahsan Shirdoust, Mehdi Azimi, Leila |
author_facet | Azimi, Taher Pourmand, Mohammad Reza Fallah, Fatemeh Karimi, Abdollah Mansour-Ghanaie, Roxana Hoseini-Alfatemi, Seyedeh Mahsan Shirdoust, Mehdi Azimi, Leila |
author_sort | Azimi, Taher |
collection | PubMed |
description | BACKGROUND: Rattus norvegicus are reservoirs for transmission of various zoonotic parasites, and they have become a threat to public health worldwide. Given the large number and the significant presence of R. norvegicus throughout the city of Tehran, this study aims to assess the frequency of zoonotic parasites carried by commensal rodents wandering in Tehran, Iran. The study considered the north, south, west, east, and center regions of Tehran for the purposes of this study. The serological tests were applied in order to detect effective antibodies against Trichomonas vaginalis (T. vaginalis), Babesia spp., and Cryptosporidium spp. using a commercial qualitative rat ELISA kit. The frequency of Toxoplasma gondii (T. gondii) was surveyed by using the conventional PCR method. Furthermore, nested PCR was employed to detect the presence of Giardia spp. and Leishmania spp. in commensal R. norvegicus dispersed in Tehran. RESULTS: Approximately, 76% of the 100 R. norvegicus tested were infected with at least one zoonotic parasite, indicating the significant frequency of parasites within the study areas. Seroreactivity against T. vaginalis, Babesia spp., and Cryptosporidium spp. was detected in 5%, 0%, and 1% of the R. norvegicus tested, respectively. T. gondii DNA was detected in 32 out of 100 (32%) R. norvegicus. In addition, Leishmania spp. and Giardia spp. DNA were found in 18 out of 100 (18%) and 76 out of 100 (76%) R. norvegicus investigated, respectively. T. vaginalis with 15% and T. gondii with 70% had the highest frequency of parasites among the R. norvegicus collected from the western and northeastern regions of Tehran, respectively. Moreover, Giardia spp. with 95% and Leishmania spp. with 30% had the highest frequency in the east and center districts, respectively. CONCLUSION: The findings showed a wide geographical dissemination of Giardia spp., Toxoplasma gondii, and Leishmania spp. in R. norvegicus within five districts of Tehran. In contrast, other parasites such as Cryptosporidium spp. infection were rarely detected in Rattus populations. No evidence for the circulation of Babesia spp. was found in this study. |
format | Online Article Text |
id | pubmed-7374959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-73749592020-07-22 Serosurvey and molecular detection of the main zoonotic parasites carried by commensal Rattus norvegicus population in Tehran, Iran Azimi, Taher Pourmand, Mohammad Reza Fallah, Fatemeh Karimi, Abdollah Mansour-Ghanaie, Roxana Hoseini-Alfatemi, Seyedeh Mahsan Shirdoust, Mehdi Azimi, Leila Trop Med Health Research BACKGROUND: Rattus norvegicus are reservoirs for transmission of various zoonotic parasites, and they have become a threat to public health worldwide. Given the large number and the significant presence of R. norvegicus throughout the city of Tehran, this study aims to assess the frequency of zoonotic parasites carried by commensal rodents wandering in Tehran, Iran. The study considered the north, south, west, east, and center regions of Tehran for the purposes of this study. The serological tests were applied in order to detect effective antibodies against Trichomonas vaginalis (T. vaginalis), Babesia spp., and Cryptosporidium spp. using a commercial qualitative rat ELISA kit. The frequency of Toxoplasma gondii (T. gondii) was surveyed by using the conventional PCR method. Furthermore, nested PCR was employed to detect the presence of Giardia spp. and Leishmania spp. in commensal R. norvegicus dispersed in Tehran. RESULTS: Approximately, 76% of the 100 R. norvegicus tested were infected with at least one zoonotic parasite, indicating the significant frequency of parasites within the study areas. Seroreactivity against T. vaginalis, Babesia spp., and Cryptosporidium spp. was detected in 5%, 0%, and 1% of the R. norvegicus tested, respectively. T. gondii DNA was detected in 32 out of 100 (32%) R. norvegicus. In addition, Leishmania spp. and Giardia spp. DNA were found in 18 out of 100 (18%) and 76 out of 100 (76%) R. norvegicus investigated, respectively. T. vaginalis with 15% and T. gondii with 70% had the highest frequency of parasites among the R. norvegicus collected from the western and northeastern regions of Tehran, respectively. Moreover, Giardia spp. with 95% and Leishmania spp. with 30% had the highest frequency in the east and center districts, respectively. CONCLUSION: The findings showed a wide geographical dissemination of Giardia spp., Toxoplasma gondii, and Leishmania spp. in R. norvegicus within five districts of Tehran. In contrast, other parasites such as Cryptosporidium spp. infection were rarely detected in Rattus populations. No evidence for the circulation of Babesia spp. was found in this study. BioMed Central 2020-07-22 /pmc/articles/PMC7374959/ /pubmed/32704233 http://dx.doi.org/10.1186/s41182-020-00246-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Azimi, Taher Pourmand, Mohammad Reza Fallah, Fatemeh Karimi, Abdollah Mansour-Ghanaie, Roxana Hoseini-Alfatemi, Seyedeh Mahsan Shirdoust, Mehdi Azimi, Leila Serosurvey and molecular detection of the main zoonotic parasites carried by commensal Rattus norvegicus population in Tehran, Iran |
title | Serosurvey and molecular detection of the main zoonotic parasites carried by commensal Rattus norvegicus population in Tehran, Iran |
title_full | Serosurvey and molecular detection of the main zoonotic parasites carried by commensal Rattus norvegicus population in Tehran, Iran |
title_fullStr | Serosurvey and molecular detection of the main zoonotic parasites carried by commensal Rattus norvegicus population in Tehran, Iran |
title_full_unstemmed | Serosurvey and molecular detection of the main zoonotic parasites carried by commensal Rattus norvegicus population in Tehran, Iran |
title_short | Serosurvey and molecular detection of the main zoonotic parasites carried by commensal Rattus norvegicus population in Tehran, Iran |
title_sort | serosurvey and molecular detection of the main zoonotic parasites carried by commensal rattus norvegicus population in tehran, iran |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374959/ https://www.ncbi.nlm.nih.gov/pubmed/32704233 http://dx.doi.org/10.1186/s41182-020-00246-3 |
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