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The role of labeled cell therapy with and without scaffold in early excision burn wounds in a rat animal model

OBJECTIVE(S): One of the essential problems in burn therapy is performing the permanent replacement of skin in full and deep thickness injuries. Human Wharton’s Jelly mesenchymal stem cells (HWJMSCs) have a unique combination of prenatal and postnatal properties. Decellularized human amniotic membra...

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Autores principales: Hashemi, Seyedeh Sara, Pourfath, Mohammad Reza, Derakhshanfar, Amin, Behzad-Behbahani, Abbas, Moayedi, Javad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374999/
https://www.ncbi.nlm.nih.gov/pubmed/32742606
http://dx.doi.org/10.22038/ijbms.2020.34324.8156
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author Hashemi, Seyedeh Sara
Pourfath, Mohammad Reza
Derakhshanfar, Amin
Behzad-Behbahani, Abbas
Moayedi, Javad
author_facet Hashemi, Seyedeh Sara
Pourfath, Mohammad Reza
Derakhshanfar, Amin
Behzad-Behbahani, Abbas
Moayedi, Javad
author_sort Hashemi, Seyedeh Sara
collection PubMed
description OBJECTIVE(S): One of the essential problems in burn therapy is performing the permanent replacement of skin in full and deep thickness injuries. Human Wharton’s Jelly mesenchymal stem cells (HWJMSCs) have a unique combination of prenatal and postnatal properties. Decellularized human amniotic membrane (DHAM) can be used as a scaffold for HWJMSCs-therapy. We aimed to evaluate the quantity and quality of healing in the early excision burn wound dressing with 3-dimensional and 2- dimensional cell cultures. MATERIALS AND METHODS: Amniotic and umbilical cords were isolated from the mothers who were candidates for cesarean section. HAM was decellularized using the mechanical and enzymatic method. HWJMSCs were isolated and cultured; cell surface markers were examined for authentication of MSCs and labeled using a viral vector containing the cGFP gene. Burns were created using brass bar in 32 adult male Albino rats and randomly divided into four groups (DHAM+HWJMSCs, injection of HWJMSCs, HWJMSCs was spread on the wound, and DHAM alone). Rats were sacrificed on the 7(th) and 14(th) days for pathological examination of the wound. Comparisons between the study groups were made by one-way analysis of variance. RESULTS: Wound healing process in DHAM+HWJMSCs was much more progressed during the first week in comparison to other groups, and exhibited significant differences in re-epithelialization, formation of granulation tissue, and hemorrhage (P<0.05). CONCLUSION: The utility of the amniotic scaffold seeded by the human mesenchymal stem cells is recommended for accelerating the healing process.
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spelling pubmed-73749992020-07-31 The role of labeled cell therapy with and without scaffold in early excision burn wounds in a rat animal model Hashemi, Seyedeh Sara Pourfath, Mohammad Reza Derakhshanfar, Amin Behzad-Behbahani, Abbas Moayedi, Javad Iran J Basic Med Sci Original Article OBJECTIVE(S): One of the essential problems in burn therapy is performing the permanent replacement of skin in full and deep thickness injuries. Human Wharton’s Jelly mesenchymal stem cells (HWJMSCs) have a unique combination of prenatal and postnatal properties. Decellularized human amniotic membrane (DHAM) can be used as a scaffold for HWJMSCs-therapy. We aimed to evaluate the quantity and quality of healing in the early excision burn wound dressing with 3-dimensional and 2- dimensional cell cultures. MATERIALS AND METHODS: Amniotic and umbilical cords were isolated from the mothers who were candidates for cesarean section. HAM was decellularized using the mechanical and enzymatic method. HWJMSCs were isolated and cultured; cell surface markers were examined for authentication of MSCs and labeled using a viral vector containing the cGFP gene. Burns were created using brass bar in 32 adult male Albino rats and randomly divided into four groups (DHAM+HWJMSCs, injection of HWJMSCs, HWJMSCs was spread on the wound, and DHAM alone). Rats were sacrificed on the 7(th) and 14(th) days for pathological examination of the wound. Comparisons between the study groups were made by one-way analysis of variance. RESULTS: Wound healing process in DHAM+HWJMSCs was much more progressed during the first week in comparison to other groups, and exhibited significant differences in re-epithelialization, formation of granulation tissue, and hemorrhage (P<0.05). CONCLUSION: The utility of the amniotic scaffold seeded by the human mesenchymal stem cells is recommended for accelerating the healing process. Mashhad University of Medical Sciences 2020-05 /pmc/articles/PMC7374999/ /pubmed/32742606 http://dx.doi.org/10.22038/ijbms.2020.34324.8156 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hashemi, Seyedeh Sara
Pourfath, Mohammad Reza
Derakhshanfar, Amin
Behzad-Behbahani, Abbas
Moayedi, Javad
The role of labeled cell therapy with and without scaffold in early excision burn wounds in a rat animal model
title The role of labeled cell therapy with and without scaffold in early excision burn wounds in a rat animal model
title_full The role of labeled cell therapy with and without scaffold in early excision burn wounds in a rat animal model
title_fullStr The role of labeled cell therapy with and without scaffold in early excision burn wounds in a rat animal model
title_full_unstemmed The role of labeled cell therapy with and without scaffold in early excision burn wounds in a rat animal model
title_short The role of labeled cell therapy with and without scaffold in early excision burn wounds in a rat animal model
title_sort role of labeled cell therapy with and without scaffold in early excision burn wounds in a rat animal model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374999/
https://www.ncbi.nlm.nih.gov/pubmed/32742606
http://dx.doi.org/10.22038/ijbms.2020.34324.8156
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