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Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix
Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaff...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7375712/ https://www.ncbi.nlm.nih.gov/pubmed/32742631 http://dx.doi.org/10.1177/2041731420921482 |
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author | Hochman-Mendez, Camila Pereira de Campos, Dilza Balteiro Pinto, Rafael Serafim Mendes, Bernardo Jorge da Silva Rocha, Gustavo Miranda Monnerat, Gustavo Weissmuller, Gilberto Sampaio, Luiz C Carvalho, Adriana Bastos Taylor, Doris A de Carvalho, Antonio Carlos Campos |
author_facet | Hochman-Mendez, Camila Pereira de Campos, Dilza Balteiro Pinto, Rafael Serafim Mendes, Bernardo Jorge da Silva Rocha, Gustavo Miranda Monnerat, Gustavo Weissmuller, Gilberto Sampaio, Luiz C Carvalho, Adriana Bastos Taylor, Doris A de Carvalho, Antonio Carlos Campos |
author_sort | Hochman-Mendez, Camila |
collection | PubMed |
description | Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (>45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue. |
format | Online Article Text |
id | pubmed-7375712 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-73757122020-07-31 Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix Hochman-Mendez, Camila Pereira de Campos, Dilza Balteiro Pinto, Rafael Serafim Mendes, Bernardo Jorge da Silva Rocha, Gustavo Miranda Monnerat, Gustavo Weissmuller, Gilberto Sampaio, Luiz C Carvalho, Adriana Bastos Taylor, Doris A de Carvalho, Antonio Carlos Campos J Tissue Eng Original Article Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (>45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue. SAGE Publications 2020-07-21 /pmc/articles/PMC7375712/ /pubmed/32742631 http://dx.doi.org/10.1177/2041731420921482 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Hochman-Mendez, Camila Pereira de Campos, Dilza Balteiro Pinto, Rafael Serafim Mendes, Bernardo Jorge da Silva Rocha, Gustavo Miranda Monnerat, Gustavo Weissmuller, Gilberto Sampaio, Luiz C Carvalho, Adriana Bastos Taylor, Doris A de Carvalho, Antonio Carlos Campos Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
title | Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
title_full | Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
title_fullStr | Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
title_full_unstemmed | Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
title_short | Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
title_sort | tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7375712/ https://www.ncbi.nlm.nih.gov/pubmed/32742631 http://dx.doi.org/10.1177/2041731420921482 |
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