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Comparative assessment of mouse models for experimental orthodontic tooth movement

Animal experiments are essential for the elucidation of biological-cellular mechanisms in the context of orthodontic tooth movement (OTM). So far, however, no studies comparatively assess available mouse models regarding their suitability. OTM of first upper molars was induced in C57BL/6 mice either...

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Autores principales: Kirschneck, Christian, Bauer, Maria, Gubernator, Joshua, Proff, Peter, Schröder, Agnes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376195/
https://www.ncbi.nlm.nih.gov/pubmed/32699355
http://dx.doi.org/10.1038/s41598-020-69030-x
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author Kirschneck, Christian
Bauer, Maria
Gubernator, Joshua
Proff, Peter
Schröder, Agnes
author_facet Kirschneck, Christian
Bauer, Maria
Gubernator, Joshua
Proff, Peter
Schröder, Agnes
author_sort Kirschneck, Christian
collection PubMed
description Animal experiments are essential for the elucidation of biological-cellular mechanisms in the context of orthodontic tooth movement (OTM). So far, however, no studies comparatively assess available mouse models regarding their suitability. OTM of first upper molars was induced in C57BL/6 mice either via an elastic band or a NiTi coil spring for three, seven or 12 days. We assessed appliance survival rate, OTM and periodontal bone loss (µCT), root resorptions, osteoclastogenesis (TRAP(+) area) and local expression of OTM-related genes (RT-qPCR). Seven days after the elastic bands were inserted, 87% were still in situ, but only 27% after 12 days. Survival rate for the NiTi coil springs was 100% throughout, but 8.9% of the animals did not survive. Both methods induced significant OTM, which was highest after 12 (NiTi spring) and 7 days (band), with a corresponding increase in local gene expression of OTM-related genes and osteoclastogenesis. Periodontal bone loss and root resorptions were not induced at a relevant extent by neither of the two procedures within the experimental periods. To induce reliable OTM in mice beyond 7 days, a NiTi coil spring is the method of choice. The elastic band method is recommended only for short-term yes/no-questions regarding OTM.
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spelling pubmed-73761952020-07-24 Comparative assessment of mouse models for experimental orthodontic tooth movement Kirschneck, Christian Bauer, Maria Gubernator, Joshua Proff, Peter Schröder, Agnes Sci Rep Article Animal experiments are essential for the elucidation of biological-cellular mechanisms in the context of orthodontic tooth movement (OTM). So far, however, no studies comparatively assess available mouse models regarding their suitability. OTM of first upper molars was induced in C57BL/6 mice either via an elastic band or a NiTi coil spring for three, seven or 12 days. We assessed appliance survival rate, OTM and periodontal bone loss (µCT), root resorptions, osteoclastogenesis (TRAP(+) area) and local expression of OTM-related genes (RT-qPCR). Seven days after the elastic bands were inserted, 87% were still in situ, but only 27% after 12 days. Survival rate for the NiTi coil springs was 100% throughout, but 8.9% of the animals did not survive. Both methods induced significant OTM, which was highest after 12 (NiTi spring) and 7 days (band), with a corresponding increase in local gene expression of OTM-related genes and osteoclastogenesis. Periodontal bone loss and root resorptions were not induced at a relevant extent by neither of the two procedures within the experimental periods. To induce reliable OTM in mice beyond 7 days, a NiTi coil spring is the method of choice. The elastic band method is recommended only for short-term yes/no-questions regarding OTM. Nature Publishing Group UK 2020-07-22 /pmc/articles/PMC7376195/ /pubmed/32699355 http://dx.doi.org/10.1038/s41598-020-69030-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kirschneck, Christian
Bauer, Maria
Gubernator, Joshua
Proff, Peter
Schröder, Agnes
Comparative assessment of mouse models for experimental orthodontic tooth movement
title Comparative assessment of mouse models for experimental orthodontic tooth movement
title_full Comparative assessment of mouse models for experimental orthodontic tooth movement
title_fullStr Comparative assessment of mouse models for experimental orthodontic tooth movement
title_full_unstemmed Comparative assessment of mouse models for experimental orthodontic tooth movement
title_short Comparative assessment of mouse models for experimental orthodontic tooth movement
title_sort comparative assessment of mouse models for experimental orthodontic tooth movement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376195/
https://www.ncbi.nlm.nih.gov/pubmed/32699355
http://dx.doi.org/10.1038/s41598-020-69030-x
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