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PAAR proteins act as the ‘sorting hat’ of the type VI secretion system

Bacteria exist in polymicrobial environments and compete to prevail in a niche. The type VI secretion system (T6SS) is a nanomachine employed by Gram-negative bacteria to deliver effector proteins into target cells. Consequently, T6SS-positive bacteria produce a wealth of antibacterial effector prot...

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Detalles Bibliográficos
Autores principales: Wood, Thomas E., Howard, Sophie A., Wettstadt, Sarah, Filloux, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376260/
https://www.ncbi.nlm.nih.gov/pubmed/31380737
http://dx.doi.org/10.1099/mic.0.000842
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author Wood, Thomas E.
Howard, Sophie A.
Wettstadt, Sarah
Filloux, Alain
author_facet Wood, Thomas E.
Howard, Sophie A.
Wettstadt, Sarah
Filloux, Alain
author_sort Wood, Thomas E.
collection PubMed
description Bacteria exist in polymicrobial environments and compete to prevail in a niche. The type VI secretion system (T6SS) is a nanomachine employed by Gram-negative bacteria to deliver effector proteins into target cells. Consequently, T6SS-positive bacteria produce a wealth of antibacterial effector proteins to promote their survival among a prokaryotic community. These toxins are loaded onto the VgrG–PAAR spike and Hcp tube of the T6SS apparatus and recent work has started to document the specificity of effectors for certain spike components. Pseudomonas aeruginosa encodes several PAAR proteins, whose roles have been poorly investigated. Here we describe a phospholipase family antibacterial effector immunity pair from Pseudomonas aeruginosa and demonstrate that a specific PAAR protein is necessary for the delivery of the effector and its cognate VgrG. Furthermore, the PAAR protein appears to restrict the delivery of other phospholipase effectors that utilise distinct VgrG proteins. We provide further evidence for competition for PAAR protein recruitment to the T6SS apparatus, which determines the identities of the delivered effectors.
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spelling pubmed-73762602020-07-24 PAAR proteins act as the ‘sorting hat’ of the type VI secretion system Wood, Thomas E. Howard, Sophie A. Wettstadt, Sarah Filloux, Alain Microbiology (Reading) Physiology and Metabolism Bacteria exist in polymicrobial environments and compete to prevail in a niche. The type VI secretion system (T6SS) is a nanomachine employed by Gram-negative bacteria to deliver effector proteins into target cells. Consequently, T6SS-positive bacteria produce a wealth of antibacterial effector proteins to promote their survival among a prokaryotic community. These toxins are loaded onto the VgrG–PAAR spike and Hcp tube of the T6SS apparatus and recent work has started to document the specificity of effectors for certain spike components. Pseudomonas aeruginosa encodes several PAAR proteins, whose roles have been poorly investigated. Here we describe a phospholipase family antibacterial effector immunity pair from Pseudomonas aeruginosa and demonstrate that a specific PAAR protein is necessary for the delivery of the effector and its cognate VgrG. Furthermore, the PAAR protein appears to restrict the delivery of other phospholipase effectors that utilise distinct VgrG proteins. We provide further evidence for competition for PAAR protein recruitment to the T6SS apparatus, which determines the identities of the delivered effectors. Microbiology Society 2019-11 2019-08-05 /pmc/articles/PMC7376260/ /pubmed/31380737 http://dx.doi.org/10.1099/mic.0.000842 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Physiology and Metabolism
Wood, Thomas E.
Howard, Sophie A.
Wettstadt, Sarah
Filloux, Alain
PAAR proteins act as the ‘sorting hat’ of the type VI secretion system
title PAAR proteins act as the ‘sorting hat’ of the type VI secretion system
title_full PAAR proteins act as the ‘sorting hat’ of the type VI secretion system
title_fullStr PAAR proteins act as the ‘sorting hat’ of the type VI secretion system
title_full_unstemmed PAAR proteins act as the ‘sorting hat’ of the type VI secretion system
title_short PAAR proteins act as the ‘sorting hat’ of the type VI secretion system
title_sort paar proteins act as the ‘sorting hat’ of the type vi secretion system
topic Physiology and Metabolism
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376260/
https://www.ncbi.nlm.nih.gov/pubmed/31380737
http://dx.doi.org/10.1099/mic.0.000842
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