Cargando…
A Circulating Long Noncoding RNA Panel Serves as a Diagnostic Marker for Hepatocellular Carcinoma
BACKGROUND: Circulating long noncoding RNAs (lncRNAs) have been demonstrated to serve as diagnostic biomarkers for various cancers. We aimed to elucidate the diagnostic efficacy of eight serum lncRNAs HULC, MALAT1, Linc00152, PTENP1, PTTG3P, SPRY4-IT1, UBE2CP3, and UCA1 and their combinations for th...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376401/ https://www.ncbi.nlm.nih.gov/pubmed/32733618 http://dx.doi.org/10.1155/2020/5417598 |
_version_ | 1783562036859371520 |
---|---|
author | Huang, Jinlan Zheng, Yansong Xiao, Xialin Liu, Can Lin, Jinpiao Zheng, Sijia Yang, Bin Ou, Qishui |
author_facet | Huang, Jinlan Zheng, Yansong Xiao, Xialin Liu, Can Lin, Jinpiao Zheng, Sijia Yang, Bin Ou, Qishui |
author_sort | Huang, Jinlan |
collection | PubMed |
description | BACKGROUND: Circulating long noncoding RNAs (lncRNAs) have been demonstrated to serve as diagnostic biomarkers for various cancers. We aimed to elucidate the diagnostic efficacy of eight serum lncRNAs HULC, MALAT1, Linc00152, PTENP1, PTTG3P, SPRY4-IT1, UBE2CP3, and UCA1 and their combinations for the diagnosis of hepatocellular carcinoma (HCC). METHODS: A total of 129 patients with HCC, 49 patients with liver cirrhosis, 27 patients with chronic hepatitis B, and 93 healthy controls were enrolled in this study. The levels of serum lncRNAs were assessed by quantitative real-time polymerase chain reaction. The correlations between serum lncRNAs and clinicopathological characteristics were further analyzed. The receiver operating characteristic (ROC) curve and area under curve (AUC) were utilized to estimate the diagnostic capacity of serum lncRNAs and their combination with AFP for HCC. A logistic regression model was performed to establish a multiple-lncRNA panel. RESULTS: The levels of serum HULC, MALAT1, Linc00152, PTTG3P, SPRY4-IT1, UBE2CP3, and UCA1 were significantly higher in HCC patients than in patients with benign liver diseases and healthy controls, whereas serum PTENP1 was significantly decreased in HCC patients compared with healthy participants. Positive correlations between serum Linc00152 and GGT, serum PTTG3P and GGT, and serum SPRY4-IT1 and ALT were noted in HCC patients. ROC analysis revealed that all these lncRNAs had a significantly predictive value for HCC except for PTENP1. The best performance of single lncRNA was obtained by Linc00152 with an AUC of 0.877. When combined with AFP, the combination of Linc00152 and AFP gained the highest accuracy, yielding an AUC of 0.906. Through logistic regression analysis, the panel consisting of serum linc00152, UCA1, and AFP provided the greatest predictive ability, obtaining an AUC of 0.912 with 82.9% sensitivity and 88.2% specificity. CONCLUSION: The panel of serum Linc00152, UCA1, and AFP demonstrates a novel and noninvasive biomarker with relatively high sensitivity and specificity for HCC diagnosis. |
format | Online Article Text |
id | pubmed-7376401 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-73764012020-07-29 A Circulating Long Noncoding RNA Panel Serves as a Diagnostic Marker for Hepatocellular Carcinoma Huang, Jinlan Zheng, Yansong Xiao, Xialin Liu, Can Lin, Jinpiao Zheng, Sijia Yang, Bin Ou, Qishui Dis Markers Research Article BACKGROUND: Circulating long noncoding RNAs (lncRNAs) have been demonstrated to serve as diagnostic biomarkers for various cancers. We aimed to elucidate the diagnostic efficacy of eight serum lncRNAs HULC, MALAT1, Linc00152, PTENP1, PTTG3P, SPRY4-IT1, UBE2CP3, and UCA1 and their combinations for the diagnosis of hepatocellular carcinoma (HCC). METHODS: A total of 129 patients with HCC, 49 patients with liver cirrhosis, 27 patients with chronic hepatitis B, and 93 healthy controls were enrolled in this study. The levels of serum lncRNAs were assessed by quantitative real-time polymerase chain reaction. The correlations between serum lncRNAs and clinicopathological characteristics were further analyzed. The receiver operating characteristic (ROC) curve and area under curve (AUC) were utilized to estimate the diagnostic capacity of serum lncRNAs and their combination with AFP for HCC. A logistic regression model was performed to establish a multiple-lncRNA panel. RESULTS: The levels of serum HULC, MALAT1, Linc00152, PTTG3P, SPRY4-IT1, UBE2CP3, and UCA1 were significantly higher in HCC patients than in patients with benign liver diseases and healthy controls, whereas serum PTENP1 was significantly decreased in HCC patients compared with healthy participants. Positive correlations between serum Linc00152 and GGT, serum PTTG3P and GGT, and serum SPRY4-IT1 and ALT were noted in HCC patients. ROC analysis revealed that all these lncRNAs had a significantly predictive value for HCC except for PTENP1. The best performance of single lncRNA was obtained by Linc00152 with an AUC of 0.877. When combined with AFP, the combination of Linc00152 and AFP gained the highest accuracy, yielding an AUC of 0.906. Through logistic regression analysis, the panel consisting of serum linc00152, UCA1, and AFP provided the greatest predictive ability, obtaining an AUC of 0.912 with 82.9% sensitivity and 88.2% specificity. CONCLUSION: The panel of serum Linc00152, UCA1, and AFP demonstrates a novel and noninvasive biomarker with relatively high sensitivity and specificity for HCC diagnosis. Hindawi 2020-07-13 /pmc/articles/PMC7376401/ /pubmed/32733618 http://dx.doi.org/10.1155/2020/5417598 Text en Copyright © 2020 Jinlan Huang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Huang, Jinlan Zheng, Yansong Xiao, Xialin Liu, Can Lin, Jinpiao Zheng, Sijia Yang, Bin Ou, Qishui A Circulating Long Noncoding RNA Panel Serves as a Diagnostic Marker for Hepatocellular Carcinoma |
title | A Circulating Long Noncoding RNA Panel Serves as a Diagnostic Marker for Hepatocellular Carcinoma |
title_full | A Circulating Long Noncoding RNA Panel Serves as a Diagnostic Marker for Hepatocellular Carcinoma |
title_fullStr | A Circulating Long Noncoding RNA Panel Serves as a Diagnostic Marker for Hepatocellular Carcinoma |
title_full_unstemmed | A Circulating Long Noncoding RNA Panel Serves as a Diagnostic Marker for Hepatocellular Carcinoma |
title_short | A Circulating Long Noncoding RNA Panel Serves as a Diagnostic Marker for Hepatocellular Carcinoma |
title_sort | circulating long noncoding rna panel serves as a diagnostic marker for hepatocellular carcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376401/ https://www.ncbi.nlm.nih.gov/pubmed/32733618 http://dx.doi.org/10.1155/2020/5417598 |
work_keys_str_mv | AT huangjinlan acirculatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT zhengyansong acirculatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT xiaoxialin acirculatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT liucan acirculatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT linjinpiao acirculatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT zhengsijia acirculatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT yangbin acirculatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT ouqishui acirculatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT huangjinlan circulatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT zhengyansong circulatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT xiaoxialin circulatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT liucan circulatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT linjinpiao circulatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT zhengsijia circulatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT yangbin circulatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma AT ouqishui circulatinglongnoncodingrnapanelservesasadiagnosticmarkerforhepatocellularcarcinoma |