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miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway

Increasing evidence has indicated that the dysregulation of microRNA (miRNA) occur in the pathogenesis of retinoblastoma (RB). Aim of the present study was to investigate the possible role of miR-494 (miR-494-3p) in RB. It was demonstrated that miR-494 expression was increased in RB tissue samples a...

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Autores principales: Xu, Fen, Liu, Guiqin, Wang, Lijuan, Wang, Xiyan, Jin, Xiao, Bo, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377044/
https://www.ncbi.nlm.nih.gov/pubmed/32724440
http://dx.doi.org/10.3892/ol.2020.11749
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author Xu, Fen
Liu, Guiqin
Wang, Lijuan
Wang, Xiyan
Jin, Xiao
Bo, Wen
author_facet Xu, Fen
Liu, Guiqin
Wang, Lijuan
Wang, Xiyan
Jin, Xiao
Bo, Wen
author_sort Xu, Fen
collection PubMed
description Increasing evidence has indicated that the dysregulation of microRNA (miRNA) occur in the pathogenesis of retinoblastoma (RB). Aim of the present study was to investigate the possible role of miR-494 (miR-494-3p) in RB. It was demonstrated that miR-494 expression was increased in RB tissue samples and cell lines. Also, it was prominently associated with clinicopathological features. Functional assays showed that RB cell proliferation, invasion and migration can be promoted by miR-494 overexpression. Besides, phosphatase and tensin homolog (PTEN) was verified as a possible target of miR-494 by a luciferase assay, western blot and qRT-PCR assay in RB. miR-494 and PTEN expression was negatively related in a correlation analysis on tumor tissues of 66 patients. In addition, PTEN was proved to reverse miR-494 effect on RB cell progression. Moreover, PI3K/AKT signaling pathway was validated to take part in RB progression. Taken together, the current study proposes that miR-494 might function as a tumor promoter and regulates RB progression through targeting PTEN.
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spelling pubmed-73770442020-07-27 miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway Xu, Fen Liu, Guiqin Wang, Lijuan Wang, Xiyan Jin, Xiao Bo, Wen Oncol Lett Articles Increasing evidence has indicated that the dysregulation of microRNA (miRNA) occur in the pathogenesis of retinoblastoma (RB). Aim of the present study was to investigate the possible role of miR-494 (miR-494-3p) in RB. It was demonstrated that miR-494 expression was increased in RB tissue samples and cell lines. Also, it was prominently associated with clinicopathological features. Functional assays showed that RB cell proliferation, invasion and migration can be promoted by miR-494 overexpression. Besides, phosphatase and tensin homolog (PTEN) was verified as a possible target of miR-494 by a luciferase assay, western blot and qRT-PCR assay in RB. miR-494 and PTEN expression was negatively related in a correlation analysis on tumor tissues of 66 patients. In addition, PTEN was proved to reverse miR-494 effect on RB cell progression. Moreover, PI3K/AKT signaling pathway was validated to take part in RB progression. Taken together, the current study proposes that miR-494 might function as a tumor promoter and regulates RB progression through targeting PTEN. D.A. Spandidos 2020-08 2020-06-17 /pmc/articles/PMC7377044/ /pubmed/32724440 http://dx.doi.org/10.3892/ol.2020.11749 Text en Copyright: © Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Xu, Fen
Liu, Guiqin
Wang, Lijuan
Wang, Xiyan
Jin, Xiao
Bo, Wen
miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway
title miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway
title_full miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway
title_fullStr miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway
title_full_unstemmed miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway
title_short miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway
title_sort mir-494 promotes progression of retinoblastoma via pten through pi3k/akt signaling pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377044/
https://www.ncbi.nlm.nih.gov/pubmed/32724440
http://dx.doi.org/10.3892/ol.2020.11749
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