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miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway
Increasing evidence has indicated that the dysregulation of microRNA (miRNA) occur in the pathogenesis of retinoblastoma (RB). Aim of the present study was to investigate the possible role of miR-494 (miR-494-3p) in RB. It was demonstrated that miR-494 expression was increased in RB tissue samples a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377044/ https://www.ncbi.nlm.nih.gov/pubmed/32724440 http://dx.doi.org/10.3892/ol.2020.11749 |
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author | Xu, Fen Liu, Guiqin Wang, Lijuan Wang, Xiyan Jin, Xiao Bo, Wen |
author_facet | Xu, Fen Liu, Guiqin Wang, Lijuan Wang, Xiyan Jin, Xiao Bo, Wen |
author_sort | Xu, Fen |
collection | PubMed |
description | Increasing evidence has indicated that the dysregulation of microRNA (miRNA) occur in the pathogenesis of retinoblastoma (RB). Aim of the present study was to investigate the possible role of miR-494 (miR-494-3p) in RB. It was demonstrated that miR-494 expression was increased in RB tissue samples and cell lines. Also, it was prominently associated with clinicopathological features. Functional assays showed that RB cell proliferation, invasion and migration can be promoted by miR-494 overexpression. Besides, phosphatase and tensin homolog (PTEN) was verified as a possible target of miR-494 by a luciferase assay, western blot and qRT-PCR assay in RB. miR-494 and PTEN expression was negatively related in a correlation analysis on tumor tissues of 66 patients. In addition, PTEN was proved to reverse miR-494 effect on RB cell progression. Moreover, PI3K/AKT signaling pathway was validated to take part in RB progression. Taken together, the current study proposes that miR-494 might function as a tumor promoter and regulates RB progression through targeting PTEN. |
format | Online Article Text |
id | pubmed-7377044 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-73770442020-07-27 miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway Xu, Fen Liu, Guiqin Wang, Lijuan Wang, Xiyan Jin, Xiao Bo, Wen Oncol Lett Articles Increasing evidence has indicated that the dysregulation of microRNA (miRNA) occur in the pathogenesis of retinoblastoma (RB). Aim of the present study was to investigate the possible role of miR-494 (miR-494-3p) in RB. It was demonstrated that miR-494 expression was increased in RB tissue samples and cell lines. Also, it was prominently associated with clinicopathological features. Functional assays showed that RB cell proliferation, invasion and migration can be promoted by miR-494 overexpression. Besides, phosphatase and tensin homolog (PTEN) was verified as a possible target of miR-494 by a luciferase assay, western blot and qRT-PCR assay in RB. miR-494 and PTEN expression was negatively related in a correlation analysis on tumor tissues of 66 patients. In addition, PTEN was proved to reverse miR-494 effect on RB cell progression. Moreover, PI3K/AKT signaling pathway was validated to take part in RB progression. Taken together, the current study proposes that miR-494 might function as a tumor promoter and regulates RB progression through targeting PTEN. D.A. Spandidos 2020-08 2020-06-17 /pmc/articles/PMC7377044/ /pubmed/32724440 http://dx.doi.org/10.3892/ol.2020.11749 Text en Copyright: © Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Xu, Fen Liu, Guiqin Wang, Lijuan Wang, Xiyan Jin, Xiao Bo, Wen miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway |
title | miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway |
title_full | miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway |
title_fullStr | miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway |
title_full_unstemmed | miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway |
title_short | miR-494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway |
title_sort | mir-494 promotes progression of retinoblastoma via pten through pi3k/akt signaling pathway |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377044/ https://www.ncbi.nlm.nih.gov/pubmed/32724440 http://dx.doi.org/10.3892/ol.2020.11749 |
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