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Establishment of C(20)Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells
CD20 is one of several B-lymphocyte antigens that has been shown to be an effective target for the detection and treatment of B-cell lymphomas. Sensitive and specific monoclonal antibodies (mAbs) are required for every application used for the diagnosis of B-cell lymphoma. Although many anti-CD20 mA...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377059/ https://www.ncbi.nlm.nih.gov/pubmed/32724441 http://dx.doi.org/10.3892/ol.2020.11753 |
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author | Furusawa, Yoshikazu Kaneko, Mika Kato Kato, Yukinari |
author_facet | Furusawa, Yoshikazu Kaneko, Mika Kato Kato, Yukinari |
author_sort | Furusawa, Yoshikazu |
collection | PubMed |
description | CD20 is one of several B-lymphocyte antigens that has been shown to be an effective target for the detection and treatment of B-cell lymphomas. Sensitive and specific monoclonal antibodies (mAbs) are required for every application used for the diagnosis of B-cell lymphoma. Although many anti-CD20 mAbs have been established, the types of applications, those anti-CD20 can be used in, are limited. In this study, we aimed to establish novel anti-CD20 mAbs to be used for broad applications, such as flow cytometry, western blot, and immunohistochemical analyses, using the Cell-Based Immunization and Screening (CBIS) method. One of the established mAbs, C(20)Mab-11 (IgM, kappa), detected overexpression of CD20 in CHO-K1 or LN229 cell lines, indicating that C(20)Mab-11 is specific for CD20. In western blot analyses, C(20)Mab-11 detected not only overexpression of CD20 in CHO-K1 or LN229 cell lines, but also CD20 of BALL-1 and Raji cells with both sensitivity and specificity. Furthermore, C(20)Mab-11 strongly stained B cells of the lymph follicle and B cell lymphomas in immunohistochemical analyses. These results indicate that C(20)Mab-11 develped by CBIS method, is useful for the detection of CD20 in lymphoma tissues by flow cytometry, western blot, and immunohistochemical analyses and potentially could be beneficial for the treatment of B cell lymphomas. |
format | Online Article Text |
id | pubmed-7377059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-73770592020-07-27 Establishment of C(20)Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells Furusawa, Yoshikazu Kaneko, Mika Kato Kato, Yukinari Oncol Lett Articles CD20 is one of several B-lymphocyte antigens that has been shown to be an effective target for the detection and treatment of B-cell lymphomas. Sensitive and specific monoclonal antibodies (mAbs) are required for every application used for the diagnosis of B-cell lymphoma. Although many anti-CD20 mAbs have been established, the types of applications, those anti-CD20 can be used in, are limited. In this study, we aimed to establish novel anti-CD20 mAbs to be used for broad applications, such as flow cytometry, western blot, and immunohistochemical analyses, using the Cell-Based Immunization and Screening (CBIS) method. One of the established mAbs, C(20)Mab-11 (IgM, kappa), detected overexpression of CD20 in CHO-K1 or LN229 cell lines, indicating that C(20)Mab-11 is specific for CD20. In western blot analyses, C(20)Mab-11 detected not only overexpression of CD20 in CHO-K1 or LN229 cell lines, but also CD20 of BALL-1 and Raji cells with both sensitivity and specificity. Furthermore, C(20)Mab-11 strongly stained B cells of the lymph follicle and B cell lymphomas in immunohistochemical analyses. These results indicate that C(20)Mab-11 develped by CBIS method, is useful for the detection of CD20 in lymphoma tissues by flow cytometry, western blot, and immunohistochemical analyses and potentially could be beneficial for the treatment of B cell lymphomas. D.A. Spandidos 2020-08 2020-06-17 /pmc/articles/PMC7377059/ /pubmed/32724441 http://dx.doi.org/10.3892/ol.2020.11753 Text en Copyright: © Furusawa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Articles Furusawa, Yoshikazu Kaneko, Mika Kato Kato, Yukinari Establishment of C(20)Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells |
title | Establishment of C(20)Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells |
title_full | Establishment of C(20)Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells |
title_fullStr | Establishment of C(20)Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells |
title_full_unstemmed | Establishment of C(20)Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells |
title_short | Establishment of C(20)Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells |
title_sort | establishment of c(20)mab-11, a novel anti-cd20 monoclonal antibody, for the detection of b cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377059/ https://www.ncbi.nlm.nih.gov/pubmed/32724441 http://dx.doi.org/10.3892/ol.2020.11753 |
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