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HLX affects cell cycle and proliferation in AML cells via the JAK/STAT signaling pathway
Acute myelogenous leukemia (AML) is a class of malignant tumors derived from hematopoietic stem or progenitor cells. The H2.0-like homeobox gene (HLX) encodes transcription factors that function in promoting normal hematopoietic cell proliferation and tumor immunity. The present study analyzed the e...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377103/ https://www.ncbi.nlm.nih.gov/pubmed/32724432 http://dx.doi.org/10.3892/ol.2020.11718 |
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author | Zhu, Xia-Yin Guo, Qun-Yi Zhu, Min Chen, Bao-Guo Wang, Ling-Yan Zhang, Dan-Qiong Zhang, Li Shao, Yan-Ping Luo, Wen-Da |
author_facet | Zhu, Xia-Yin Guo, Qun-Yi Zhu, Min Chen, Bao-Guo Wang, Ling-Yan Zhang, Dan-Qiong Zhang, Li Shao, Yan-Ping Luo, Wen-Da |
author_sort | Zhu, Xia-Yin |
collection | PubMed |
description | Acute myelogenous leukemia (AML) is a class of malignant tumors derived from hematopoietic stem or progenitor cells. The H2.0-like homeobox gene (HLX) encodes transcription factors that function in promoting normal hematopoietic cell proliferation and tumor immunity. The present study analyzed the effect of downregulating the HLX on cell cycle distribution and cell proliferation in AML. Moreover, the current study detected changes in the expression of genes and proteins in the Janus kinase (JAK)/STAT signaling pathway to investigate the mechanism of the action of HLX in tumor immunity in AML. HLX expression in AML cell lines was silenced using small interfering siRNA, and MTS/PMS-assay colorimetric assays were used to assess the effect of knockdown of HLX on AML cell proliferation. Flow cytometry was used to analyze changes in cell cycle distribution, while reverse transcription-quantitative PCR and western blotting were used to detect changes in the expression levels of key components of the JAK/STAT signaling pathway, such as p21-activated kinase 1 (PAK1), neuropilin 1 (NRP1), B-cell translocation gene 1 (BTG1) and STAT5. It was found that HLX was differentially expressed in AML cell lines of various subtypes, and HLX expression was higher in the AML/M3 subtype NB4 cell line compared with the control group. Knockdown of HLX in NB4 cells significantly inhibited cell proliferation and arrested cells in the G(0)/G(1) phase. Moreover, STAT5 protein expression, as well as NRP1 and PAK1 expression levels were downregulated, while BTG1 expression was upregulated when HLX was knocked out by siRNA. Collectively, the results suggested that downregulation of HLX may cause G(0)/G(1) phase arrest and inhibit the proliferation of AML cells by activating the JAK/STAT signaling pathway. |
format | Online Article Text |
id | pubmed-7377103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-73771032020-07-27 HLX affects cell cycle and proliferation in AML cells via the JAK/STAT signaling pathway Zhu, Xia-Yin Guo, Qun-Yi Zhu, Min Chen, Bao-Guo Wang, Ling-Yan Zhang, Dan-Qiong Zhang, Li Shao, Yan-Ping Luo, Wen-Da Oncol Lett Articles Acute myelogenous leukemia (AML) is a class of malignant tumors derived from hematopoietic stem or progenitor cells. The H2.0-like homeobox gene (HLX) encodes transcription factors that function in promoting normal hematopoietic cell proliferation and tumor immunity. The present study analyzed the effect of downregulating the HLX on cell cycle distribution and cell proliferation in AML. Moreover, the current study detected changes in the expression of genes and proteins in the Janus kinase (JAK)/STAT signaling pathway to investigate the mechanism of the action of HLX in tumor immunity in AML. HLX expression in AML cell lines was silenced using small interfering siRNA, and MTS/PMS-assay colorimetric assays were used to assess the effect of knockdown of HLX on AML cell proliferation. Flow cytometry was used to analyze changes in cell cycle distribution, while reverse transcription-quantitative PCR and western blotting were used to detect changes in the expression levels of key components of the JAK/STAT signaling pathway, such as p21-activated kinase 1 (PAK1), neuropilin 1 (NRP1), B-cell translocation gene 1 (BTG1) and STAT5. It was found that HLX was differentially expressed in AML cell lines of various subtypes, and HLX expression was higher in the AML/M3 subtype NB4 cell line compared with the control group. Knockdown of HLX in NB4 cells significantly inhibited cell proliferation and arrested cells in the G(0)/G(1) phase. Moreover, STAT5 protein expression, as well as NRP1 and PAK1 expression levels were downregulated, while BTG1 expression was upregulated when HLX was knocked out by siRNA. Collectively, the results suggested that downregulation of HLX may cause G(0)/G(1) phase arrest and inhibit the proliferation of AML cells by activating the JAK/STAT signaling pathway. D.A. Spandidos 2020-08 2020-06-09 /pmc/articles/PMC7377103/ /pubmed/32724432 http://dx.doi.org/10.3892/ol.2020.11718 Text en Copyright: © Zhu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhu, Xia-Yin Guo, Qun-Yi Zhu, Min Chen, Bao-Guo Wang, Ling-Yan Zhang, Dan-Qiong Zhang, Li Shao, Yan-Ping Luo, Wen-Da HLX affects cell cycle and proliferation in AML cells via the JAK/STAT signaling pathway |
title | HLX affects cell cycle and proliferation in AML cells via the JAK/STAT signaling pathway |
title_full | HLX affects cell cycle and proliferation in AML cells via the JAK/STAT signaling pathway |
title_fullStr | HLX affects cell cycle and proliferation in AML cells via the JAK/STAT signaling pathway |
title_full_unstemmed | HLX affects cell cycle and proliferation in AML cells via the JAK/STAT signaling pathway |
title_short | HLX affects cell cycle and proliferation in AML cells via the JAK/STAT signaling pathway |
title_sort | hlx affects cell cycle and proliferation in aml cells via the jak/stat signaling pathway |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377103/ https://www.ncbi.nlm.nih.gov/pubmed/32724432 http://dx.doi.org/10.3892/ol.2020.11718 |
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