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Proteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin
[Image: see text] Trypsin is the most used enzyme in proteomics. Nevertheless, proteases with complementary cleavage specificity have been applied in special circumstances. In this work, we analyzed the characteristics of five protease alternatives to trypsin for protein identification and sequence...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American
Chemical
Society
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377536/ https://www.ncbi.nlm.nih.gov/pubmed/32628831 http://dx.doi.org/10.1021/acs.analchem.0c00478 |