Investigation of Anti-SARS-CoV-2 IgG and IgM Antibodies in the Patients with COVID-19 by Three Different ELISA Test Kits

We examined anti-SARS-CoV-2 IgG and IgM antibodies in 45 serum samples from 26 patients with COVID-19, who were admitted in our hospital by using three different ELISA kits. All patients had pneumonia at admission, and 7 patients required mechanical ventilator support and grouped in severe case. Ant...

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Autores principales: Nagasawa, Masayuki, Yamaguchi, Yoshimi, Furuya, Maki, Takahashi, Yui, Taki, Reiko, Nagata, Kaoru, Suzaki, Shinichiro, Kurosaki, Masayuki, Izumi, Namiki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2020
Materias:
Covid-19
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377663/
https://www.ncbi.nlm.nih.gov/pubmed/32838175
http://dx.doi.org/10.1007/s42399-020-00409-7
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author Nagasawa, Masayuki
Yamaguchi, Yoshimi
Furuya, Maki
Takahashi, Yui
Taki, Reiko
Nagata, Kaoru
Suzaki, Shinichiro
Kurosaki, Masayuki
Izumi, Namiki
author_facet Nagasawa, Masayuki
Yamaguchi, Yoshimi
Furuya, Maki
Takahashi, Yui
Taki, Reiko
Nagata, Kaoru
Suzaki, Shinichiro
Kurosaki, Masayuki
Izumi, Namiki
author_sort Nagasawa, Masayuki
collection PubMed
description We examined anti-SARS-CoV-2 IgG and IgM antibodies in 45 serum samples from 26 patients with COVID-19, who were admitted in our hospital by using three different ELISA kits. All patients had pneumonia at admission, and 7 patients required mechanical ventilator support and grouped in severe case. Anti-SARS-CoV-2 IgG and IgM antibodies turned to be partially positive between the 6th and 10th days, more than 84% positive between the 11th and 15th days, and 100% after the 16th day. One ELISA kit revealed poorer sensitivity for anti-SARS-CoV-2 IgM antibody. Negative conversion of IgM antibody was not observed in the 30th day in our cohort. All three ELISA kits showed no false positive reaction for negative serum samples. Between severe and moderate cases, there was no significant difference in the trends of anti-SARS-CoV-2 IgG and IgM antibody.
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spelling pubmed-73776632020-07-24 Investigation of Anti-SARS-CoV-2 IgG and IgM Antibodies in the Patients with COVID-19 by Three Different ELISA Test Kits Nagasawa, Masayuki Yamaguchi, Yoshimi Furuya, Maki Takahashi, Yui Taki, Reiko Nagata, Kaoru Suzaki, Shinichiro Kurosaki, Masayuki Izumi, Namiki SN Compr Clin Med Covid-19 We examined anti-SARS-CoV-2 IgG and IgM antibodies in 45 serum samples from 26 patients with COVID-19, who were admitted in our hospital by using three different ELISA kits. All patients had pneumonia at admission, and 7 patients required mechanical ventilator support and grouped in severe case. Anti-SARS-CoV-2 IgG and IgM antibodies turned to be partially positive between the 6th and 10th days, more than 84% positive between the 11th and 15th days, and 100% after the 16th day. One ELISA kit revealed poorer sensitivity for anti-SARS-CoV-2 IgM antibody. Negative conversion of IgM antibody was not observed in the 30th day in our cohort. All three ELISA kits showed no false positive reaction for negative serum samples. Between severe and moderate cases, there was no significant difference in the trends of anti-SARS-CoV-2 IgG and IgM antibody. Springer International Publishing 2020-07-23 2020 /pmc/articles/PMC7377663/ /pubmed/32838175 http://dx.doi.org/10.1007/s42399-020-00409-7 Text en © Springer Nature Switzerland AG 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Covid-19
Nagasawa, Masayuki
Yamaguchi, Yoshimi
Furuya, Maki
Takahashi, Yui
Taki, Reiko
Nagata, Kaoru
Suzaki, Shinichiro
Kurosaki, Masayuki
Izumi, Namiki
Investigation of Anti-SARS-CoV-2 IgG and IgM Antibodies in the Patients with COVID-19 by Three Different ELISA Test Kits
title Investigation of Anti-SARS-CoV-2 IgG and IgM Antibodies in the Patients with COVID-19 by Three Different ELISA Test Kits
title_full Investigation of Anti-SARS-CoV-2 IgG and IgM Antibodies in the Patients with COVID-19 by Three Different ELISA Test Kits
title_fullStr Investigation of Anti-SARS-CoV-2 IgG and IgM Antibodies in the Patients with COVID-19 by Three Different ELISA Test Kits
title_full_unstemmed Investigation of Anti-SARS-CoV-2 IgG and IgM Antibodies in the Patients with COVID-19 by Three Different ELISA Test Kits
title_short Investigation of Anti-SARS-CoV-2 IgG and IgM Antibodies in the Patients with COVID-19 by Three Different ELISA Test Kits
title_sort investigation of anti-sars-cov-2 igg and igm antibodies in the patients with covid-19 by three different elisa test kits
topic Covid-19
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377663/
https://www.ncbi.nlm.nih.gov/pubmed/32838175
http://dx.doi.org/10.1007/s42399-020-00409-7
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