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Molecular detection of Coxiella burnetii in horse sera in Iran
Coxiella burnetii is a zoonotic bacterium that can infect a wide range of animals including horses. However, its circulation dynamics in and through horses are still unclear. The aim of this study was to evaluate prevalence of C. burnetii and its genomic characteristics in horse sera samples in the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377784/ https://www.ncbi.nlm.nih.gov/pubmed/32721772 http://dx.doi.org/10.1016/j.cimid.2020.101521 |
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author | Khademi, P Ownagh, A Ataei, B Kazemnia, A Eydi, J Khalili, M M, Mahzounieh Mardani, K |
author_facet | Khademi, P Ownagh, A Ataei, B Kazemnia, A Eydi, J Khalili, M M, Mahzounieh Mardani, K |
author_sort | Khademi, P |
collection | PubMed |
description | Coxiella burnetii is a zoonotic bacterium that can infect a wide range of animals including horses. However, its circulation dynamics in and through horses are still unclear. The aim of this study was to evaluate prevalence of C. burnetii and its genomic characteristics in horse sera samples in the North of Iran (Golestan Province). The samples were collected in 2018 and the age, sex, and breed of each animal were recorded. Nested-PCR was used to detect C. burnetii based on the presence of the transposable gene IS1111. The results showed that 7.50 % (P < 0.05; 95 % CI: 0.5 %–0.12 %) of the examined sera samples were positive for C. burnetii. Based on the resuls, prevalence of C. burnetii in the age groupof < Years 1–5 (p-value <0.05, 95 % CI: 1 %–8 %) was less than the age group of >6 years old (p-value <0.05, 95 %, CI: 7 %–19.8 %). In previous studies, it was concluded that the horses' population in Golestan Province should be considered as an important factor in the epidemiology of Q fever and consequently in public health. Further studies should be implemented to evaluate if horses may be relevant indicators of zoonotic risk in urban and suburban endemic areas. |
format | Online Article Text |
id | pubmed-7377784 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73777842020-07-24 Molecular detection of Coxiella burnetii in horse sera in Iran Khademi, P Ownagh, A Ataei, B Kazemnia, A Eydi, J Khalili, M M, Mahzounieh Mardani, K Comp Immunol Microbiol Infect Dis Article Coxiella burnetii is a zoonotic bacterium that can infect a wide range of animals including horses. However, its circulation dynamics in and through horses are still unclear. The aim of this study was to evaluate prevalence of C. burnetii and its genomic characteristics in horse sera samples in the North of Iran (Golestan Province). The samples were collected in 2018 and the age, sex, and breed of each animal were recorded. Nested-PCR was used to detect C. burnetii based on the presence of the transposable gene IS1111. The results showed that 7.50 % (P < 0.05; 95 % CI: 0.5 %–0.12 %) of the examined sera samples were positive for C. burnetii. Based on the resuls, prevalence of C. burnetii in the age groupof < Years 1–5 (p-value <0.05, 95 % CI: 1 %–8 %) was less than the age group of >6 years old (p-value <0.05, 95 %, CI: 7 %–19.8 %). In previous studies, it was concluded that the horses' population in Golestan Province should be considered as an important factor in the epidemiology of Q fever and consequently in public health. Further studies should be implemented to evaluate if horses may be relevant indicators of zoonotic risk in urban and suburban endemic areas. Elsevier Ltd. 2020-10 2020-07-23 /pmc/articles/PMC7377784/ /pubmed/32721772 http://dx.doi.org/10.1016/j.cimid.2020.101521 Text en © 2020 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Khademi, P Ownagh, A Ataei, B Kazemnia, A Eydi, J Khalili, M M, Mahzounieh Mardani, K Molecular detection of Coxiella burnetii in horse sera in Iran |
title | Molecular detection of Coxiella burnetii in horse sera in Iran |
title_full | Molecular detection of Coxiella burnetii in horse sera in Iran |
title_fullStr | Molecular detection of Coxiella burnetii in horse sera in Iran |
title_full_unstemmed | Molecular detection of Coxiella burnetii in horse sera in Iran |
title_short | Molecular detection of Coxiella burnetii in horse sera in Iran |
title_sort | molecular detection of coxiella burnetii in horse sera in iran |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377784/ https://www.ncbi.nlm.nih.gov/pubmed/32721772 http://dx.doi.org/10.1016/j.cimid.2020.101521 |
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