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Altered circular RNA expression profiles in the non-ischemic thalamus in focal cortical infarction mice

Focal cerebral infarction leads to secondary changes in non-ischemic areas remote from but connected to the infarct site. Circular RNAs (circRNAs) are involved in the pathophysiological processes of many diseases. However, the expression and roles of circRNAs in non-ischemic remote regions after isc...

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Autores principales: Li, Fangming, Li, Chuqiao, Li, Xiaoqiang, Li, Yudi, Zhong, Yulan, Ling, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377861/
https://www.ncbi.nlm.nih.gov/pubmed/32639948
http://dx.doi.org/10.18632/aging.103424
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author Li, Fangming
Li, Chuqiao
Li, Xiaoqiang
Li, Yudi
Zhong, Yulan
Ling, Li
author_facet Li, Fangming
Li, Chuqiao
Li, Xiaoqiang
Li, Yudi
Zhong, Yulan
Ling, Li
author_sort Li, Fangming
collection PubMed
description Focal cerebral infarction leads to secondary changes in non-ischemic areas remote from but connected to the infarct site. Circular RNAs (circRNAs) are involved in the pathophysiological processes of many diseases. However, the expression and roles of circRNAs in non-ischemic remote regions after ischemic stroke remain unknown. In this study, adult male C57BL/6J mice were subjected to permanent distal middle cerebral artery occlusion (MCAO) to establish focal cortical infarction. High-throughput sequencing was used to profile the circRNA expression in the mouse ipsilateral thalamus at 7 and 14 d after MCAO. Bioinformatics analyses were conducted to predict the function of the differential expressed circRNAs’ host and target genes. Compared with sham group, a total of 2659 circRNAs were significantly altered in the ipsilateral thalamus at 7 or 14 d after MCAO in mice. Among them, 73 circRNAs were significantly altered at both two time points after stroke. GO and KEGG analyses indicated that circRNAs plays important roles in secondary thalamic neurodegeneration and remodeling after focal cortical infarction. This is the first study to profile the circRNA expression in non-ischemic region of ischemic stroke, suggesting that circRNAs may be therapeutic targets for reducing post-stroke secondary remote neurodegeneration.
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spelling pubmed-73778612020-07-31 Altered circular RNA expression profiles in the non-ischemic thalamus in focal cortical infarction mice Li, Fangming Li, Chuqiao Li, Xiaoqiang Li, Yudi Zhong, Yulan Ling, Li Aging (Albany NY) Research Paper Focal cerebral infarction leads to secondary changes in non-ischemic areas remote from but connected to the infarct site. Circular RNAs (circRNAs) are involved in the pathophysiological processes of many diseases. However, the expression and roles of circRNAs in non-ischemic remote regions after ischemic stroke remain unknown. In this study, adult male C57BL/6J mice were subjected to permanent distal middle cerebral artery occlusion (MCAO) to establish focal cortical infarction. High-throughput sequencing was used to profile the circRNA expression in the mouse ipsilateral thalamus at 7 and 14 d after MCAO. Bioinformatics analyses were conducted to predict the function of the differential expressed circRNAs’ host and target genes. Compared with sham group, a total of 2659 circRNAs were significantly altered in the ipsilateral thalamus at 7 or 14 d after MCAO in mice. Among them, 73 circRNAs were significantly altered at both two time points after stroke. GO and KEGG analyses indicated that circRNAs plays important roles in secondary thalamic neurodegeneration and remodeling after focal cortical infarction. This is the first study to profile the circRNA expression in non-ischemic region of ischemic stroke, suggesting that circRNAs may be therapeutic targets for reducing post-stroke secondary remote neurodegeneration. Impact Journals 2020-07-08 /pmc/articles/PMC7377861/ /pubmed/32639948 http://dx.doi.org/10.18632/aging.103424 Text en Copyright © 2020 Li et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Li, Fangming
Li, Chuqiao
Li, Xiaoqiang
Li, Yudi
Zhong, Yulan
Ling, Li
Altered circular RNA expression profiles in the non-ischemic thalamus in focal cortical infarction mice
title Altered circular RNA expression profiles in the non-ischemic thalamus in focal cortical infarction mice
title_full Altered circular RNA expression profiles in the non-ischemic thalamus in focal cortical infarction mice
title_fullStr Altered circular RNA expression profiles in the non-ischemic thalamus in focal cortical infarction mice
title_full_unstemmed Altered circular RNA expression profiles in the non-ischemic thalamus in focal cortical infarction mice
title_short Altered circular RNA expression profiles in the non-ischemic thalamus in focal cortical infarction mice
title_sort altered circular rna expression profiles in the non-ischemic thalamus in focal cortical infarction mice
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377861/
https://www.ncbi.nlm.nih.gov/pubmed/32639948
http://dx.doi.org/10.18632/aging.103424
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