Cargando…

MicroRNA-124 inhibits macrophage cell apoptosis via targeting p38/MAPK signaling pathway in atherosclerosis development

The objective of this study is to characterize the function of microRNA (miR)-124 in the process of coronary artery disease (CAD). Eighty patients, including 40 CAD patients and 40 non-CAD control patients were enrolled in this study. Atherosclerosis model was established in vivo in ApoE-/- mice and...

Descripción completa

Detalles Bibliográficos
Autores principales: Liang, Xue, Wang, Lijun, Wang, Manman, Liu, Zhaohong, Liu, Xing, Zhang, Baoshuai, Liu, Enzhao, Li, Guangping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377888/
https://www.ncbi.nlm.nih.gov/pubmed/32611832
http://dx.doi.org/10.18632/aging.103387
_version_ 1783562303678971904
author Liang, Xue
Wang, Lijun
Wang, Manman
Liu, Zhaohong
Liu, Xing
Zhang, Baoshuai
Liu, Enzhao
Li, Guangping
author_facet Liang, Xue
Wang, Lijun
Wang, Manman
Liu, Zhaohong
Liu, Xing
Zhang, Baoshuai
Liu, Enzhao
Li, Guangping
author_sort Liang, Xue
collection PubMed
description The objective of this study is to characterize the function of microRNA (miR)-124 in the process of coronary artery disease (CAD). Eighty patients, including 40 CAD patients and 40 non-CAD control patients were enrolled in this study. Atherosclerosis model was established in vivo in ApoE-/- mice and in vitro in RAW264.7 cells. Expression of miR-124 and p38 in patients, animal models and cell models were measured by qRT-PCR, western blot and immunohistochemistry assay. Overexpression or suppression of miR-124 was introduced in vitro and in vivo and the expression levels of p38, miR-124, pro- and anti-inflammatory cytokines, and pro- and anti-apoptotic factors were examined. Results showed that miR-124 was decreased, while p38 was increased in CAD patients and atherosclerosis models compared with control group. MiR-124 could target p38 by binding its 3’ untranslated region and negatively regulated the protein expression of p38. Overexpression of miR-124 increased the expression of anti-inflammatory cytokines, reduced the expression of pro- inflammatory cytokines, and inhibited macrophage apoptosis. MiR-124 overexpression may be a promising treatment for atherosclerosis and CAD via inhibiting p38.
format Online
Article
Text
id pubmed-7377888
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Impact Journals
record_format MEDLINE/PubMed
spelling pubmed-73778882020-07-31 MicroRNA-124 inhibits macrophage cell apoptosis via targeting p38/MAPK signaling pathway in atherosclerosis development Liang, Xue Wang, Lijun Wang, Manman Liu, Zhaohong Liu, Xing Zhang, Baoshuai Liu, Enzhao Li, Guangping Aging (Albany NY) Research Paper The objective of this study is to characterize the function of microRNA (miR)-124 in the process of coronary artery disease (CAD). Eighty patients, including 40 CAD patients and 40 non-CAD control patients were enrolled in this study. Atherosclerosis model was established in vivo in ApoE-/- mice and in vitro in RAW264.7 cells. Expression of miR-124 and p38 in patients, animal models and cell models were measured by qRT-PCR, western blot and immunohistochemistry assay. Overexpression or suppression of miR-124 was introduced in vitro and in vivo and the expression levels of p38, miR-124, pro- and anti-inflammatory cytokines, and pro- and anti-apoptotic factors were examined. Results showed that miR-124 was decreased, while p38 was increased in CAD patients and atherosclerosis models compared with control group. MiR-124 could target p38 by binding its 3’ untranslated region and negatively regulated the protein expression of p38. Overexpression of miR-124 increased the expression of anti-inflammatory cytokines, reduced the expression of pro- inflammatory cytokines, and inhibited macrophage apoptosis. MiR-124 overexpression may be a promising treatment for atherosclerosis and CAD via inhibiting p38. Impact Journals 2020-06-30 /pmc/articles/PMC7377888/ /pubmed/32611832 http://dx.doi.org/10.18632/aging.103387 Text en Copyright © 2020 Liang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Liang, Xue
Wang, Lijun
Wang, Manman
Liu, Zhaohong
Liu, Xing
Zhang, Baoshuai
Liu, Enzhao
Li, Guangping
MicroRNA-124 inhibits macrophage cell apoptosis via targeting p38/MAPK signaling pathway in atherosclerosis development
title MicroRNA-124 inhibits macrophage cell apoptosis via targeting p38/MAPK signaling pathway in atherosclerosis development
title_full MicroRNA-124 inhibits macrophage cell apoptosis via targeting p38/MAPK signaling pathway in atherosclerosis development
title_fullStr MicroRNA-124 inhibits macrophage cell apoptosis via targeting p38/MAPK signaling pathway in atherosclerosis development
title_full_unstemmed MicroRNA-124 inhibits macrophage cell apoptosis via targeting p38/MAPK signaling pathway in atherosclerosis development
title_short MicroRNA-124 inhibits macrophage cell apoptosis via targeting p38/MAPK signaling pathway in atherosclerosis development
title_sort microrna-124 inhibits macrophage cell apoptosis via targeting p38/mapk signaling pathway in atherosclerosis development
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377888/
https://www.ncbi.nlm.nih.gov/pubmed/32611832
http://dx.doi.org/10.18632/aging.103387
work_keys_str_mv AT liangxue microrna124inhibitsmacrophagecellapoptosisviatargetingp38mapksignalingpathwayinatherosclerosisdevelopment
AT wanglijun microrna124inhibitsmacrophagecellapoptosisviatargetingp38mapksignalingpathwayinatherosclerosisdevelopment
AT wangmanman microrna124inhibitsmacrophagecellapoptosisviatargetingp38mapksignalingpathwayinatherosclerosisdevelopment
AT liuzhaohong microrna124inhibitsmacrophagecellapoptosisviatargetingp38mapksignalingpathwayinatherosclerosisdevelopment
AT liuxing microrna124inhibitsmacrophagecellapoptosisviatargetingp38mapksignalingpathwayinatherosclerosisdevelopment
AT zhangbaoshuai microrna124inhibitsmacrophagecellapoptosisviatargetingp38mapksignalingpathwayinatherosclerosisdevelopment
AT liuenzhao microrna124inhibitsmacrophagecellapoptosisviatargetingp38mapksignalingpathwayinatherosclerosisdevelopment
AT liguangping microrna124inhibitsmacrophagecellapoptosisviatargetingp38mapksignalingpathwayinatherosclerosisdevelopment