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CircFOXO3 rs12196996, a polymorphism at the gene flanking intron, is associated with circFOXO3 levels and the risk of coronary artery disease

CircFOXO3 plays an important role in the pathogenesis of coronary artery disease (CAD). Single nucleotide polymorphisms (SNPs) at circRNA flanking introns may change its back-splicing and influence circRNA formation. Here, we aimed to investigate the influence of the polymorphisms at the circFOXO3 f...

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Autores principales: Zhou, Yu-Lan, Wu, Wei-Peng, Cheng, Jie, Liang, Li-Li, Cen, Jin-Ming, Chen, Can, Liu, Xinguang, Xiong, Xing-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377899/
https://www.ncbi.nlm.nih.gov/pubmed/32614786
http://dx.doi.org/10.18632/aging.103398
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author Zhou, Yu-Lan
Wu, Wei-Peng
Cheng, Jie
Liang, Li-Li
Cen, Jin-Ming
Chen, Can
Liu, Xinguang
Xiong, Xing-Dong
author_facet Zhou, Yu-Lan
Wu, Wei-Peng
Cheng, Jie
Liang, Li-Li
Cen, Jin-Ming
Chen, Can
Liu, Xinguang
Xiong, Xing-Dong
author_sort Zhou, Yu-Lan
collection PubMed
description CircFOXO3 plays an important role in the pathogenesis of coronary artery disease (CAD). Single nucleotide polymorphisms (SNPs) at circRNA flanking introns may change its back-splicing and influence circRNA formation. Here, we aimed to investigate the influence of the polymorphisms at the circFOXO3 flanking introns on individual susceptibility to CAD. A total of 1185 individuals were included in the case-control study. In a multivariate logistic regression analysis, we determined that the rs12196996 G variant was significantly associated with increased CAD risk (OR = 1.36, P = 0.014). A similar trend of the association was observed in the recessive model (OR = 2.57, P = 0.003). Stratified analysis revealed a more significant association with CAD risk among younger subjects and non-smokers. Consistent with these results, the haplotype rs12196996G-rs9398171C containing rs12196996G allele was also associated with increased CAD risk (OR = 1.31, P = 0.013). Further investigation revealed that the rs12196996 GG genotype was associated with decreased circFOXO3 expression, but not linear FOXO3 levels. Taken together, our data provide the first evidence that the rs12196996 polymorphism at the circFOXO3 gene flanking intron is associated with CAD risk in the Chinese Han population, which is probably due to influence circFOXO3 levels.
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spelling pubmed-73778992020-07-31 CircFOXO3 rs12196996, a polymorphism at the gene flanking intron, is associated with circFOXO3 levels and the risk of coronary artery disease Zhou, Yu-Lan Wu, Wei-Peng Cheng, Jie Liang, Li-Li Cen, Jin-Ming Chen, Can Liu, Xinguang Xiong, Xing-Dong Aging (Albany NY) Research Paper CircFOXO3 plays an important role in the pathogenesis of coronary artery disease (CAD). Single nucleotide polymorphisms (SNPs) at circRNA flanking introns may change its back-splicing and influence circRNA formation. Here, we aimed to investigate the influence of the polymorphisms at the circFOXO3 flanking introns on individual susceptibility to CAD. A total of 1185 individuals were included in the case-control study. In a multivariate logistic regression analysis, we determined that the rs12196996 G variant was significantly associated with increased CAD risk (OR = 1.36, P = 0.014). A similar trend of the association was observed in the recessive model (OR = 2.57, P = 0.003). Stratified analysis revealed a more significant association with CAD risk among younger subjects and non-smokers. Consistent with these results, the haplotype rs12196996G-rs9398171C containing rs12196996G allele was also associated with increased CAD risk (OR = 1.31, P = 0.013). Further investigation revealed that the rs12196996 GG genotype was associated with decreased circFOXO3 expression, but not linear FOXO3 levels. Taken together, our data provide the first evidence that the rs12196996 polymorphism at the circFOXO3 gene flanking intron is associated with CAD risk in the Chinese Han population, which is probably due to influence circFOXO3 levels. Impact Journals 2020-07-02 /pmc/articles/PMC7377899/ /pubmed/32614786 http://dx.doi.org/10.18632/aging.103398 Text en Copyright © 2020 Zhou et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zhou, Yu-Lan
Wu, Wei-Peng
Cheng, Jie
Liang, Li-Li
Cen, Jin-Ming
Chen, Can
Liu, Xinguang
Xiong, Xing-Dong
CircFOXO3 rs12196996, a polymorphism at the gene flanking intron, is associated with circFOXO3 levels and the risk of coronary artery disease
title CircFOXO3 rs12196996, a polymorphism at the gene flanking intron, is associated with circFOXO3 levels and the risk of coronary artery disease
title_full CircFOXO3 rs12196996, a polymorphism at the gene flanking intron, is associated with circFOXO3 levels and the risk of coronary artery disease
title_fullStr CircFOXO3 rs12196996, a polymorphism at the gene flanking intron, is associated with circFOXO3 levels and the risk of coronary artery disease
title_full_unstemmed CircFOXO3 rs12196996, a polymorphism at the gene flanking intron, is associated with circFOXO3 levels and the risk of coronary artery disease
title_short CircFOXO3 rs12196996, a polymorphism at the gene flanking intron, is associated with circFOXO3 levels and the risk of coronary artery disease
title_sort circfoxo3 rs12196996, a polymorphism at the gene flanking intron, is associated with circfoxo3 levels and the risk of coronary artery disease
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377899/
https://www.ncbi.nlm.nih.gov/pubmed/32614786
http://dx.doi.org/10.18632/aging.103398
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