Cargando…
A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling
Insect pathogens have adopted an array of mechanisms to subvert the immune pathways of their respective hosts. Suppression may occur directly at the level of host–pathogen interactions, for instance phagocytic capacity or phenoloxidase activation, or at the upstream signaling pathways that regulate...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378173/ https://www.ncbi.nlm.nih.gov/pubmed/32704134 http://dx.doi.org/10.1038/s41598-020-69306-2 |
_version_ | 1783562357531738112 |
---|---|
author | Kenney, Eric Yaparla, Amulya Hawdon, John M. O’Halloran, Damien M. Grayfer, Leon Eleftherianos, Ioannis |
author_facet | Kenney, Eric Yaparla, Amulya Hawdon, John M. O’Halloran, Damien M. Grayfer, Leon Eleftherianos, Ioannis |
author_sort | Kenney, Eric |
collection | PubMed |
description | Insect pathogens have adopted an array of mechanisms to subvert the immune pathways of their respective hosts. Suppression may occur directly at the level of host–pathogen interactions, for instance phagocytic capacity or phenoloxidase activation, or at the upstream signaling pathways that regulate these immune effectors. Insect pathogens of the family Baculoviridae, for example, are known to produce a UDP-glycosyltransferase (UGT) that negatively regulates ecdysone signaling. Normally, ecdysone positively regulates both molting and antimicrobial peptide production, so the inactivation of ecdysone by glycosylation results in a failure of host larvae to molt, and probably a reduced antimicrobial response. Here, we examine a putative ecdysteroid glycosyltransferase, Hba_07292 (Hb-ugt-1), which was previously identified in the hemolymph-activated transcriptome of the entomopathogenic nematode Heterorhabditis bacteriophora. Injection of recombinant Hb-ugt-1 (rHb-ugt-1) into Drosophila melanogaster flies resulted in diminished upregulation of antimicrobial peptides associated with both the Toll and Immune deficiency pathways. Ecdysone was implicated in this suppression by a reduction in Broad Complex expression and reduced pupation rates in r Hb-ugt-1-injected larvae. In addition to the finding that H. bacteriophora excreted-secreted products contain glycosyltransferase activity, these results demonstrate that Hb-ugt-1 is an immunosuppressive factor and that its activity likely involves the inactivation of ecdysone. |
format | Online Article Text |
id | pubmed-7378173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73781732020-07-24 A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling Kenney, Eric Yaparla, Amulya Hawdon, John M. O’Halloran, Damien M. Grayfer, Leon Eleftherianos, Ioannis Sci Rep Article Insect pathogens have adopted an array of mechanisms to subvert the immune pathways of their respective hosts. Suppression may occur directly at the level of host–pathogen interactions, for instance phagocytic capacity or phenoloxidase activation, or at the upstream signaling pathways that regulate these immune effectors. Insect pathogens of the family Baculoviridae, for example, are known to produce a UDP-glycosyltransferase (UGT) that negatively regulates ecdysone signaling. Normally, ecdysone positively regulates both molting and antimicrobial peptide production, so the inactivation of ecdysone by glycosylation results in a failure of host larvae to molt, and probably a reduced antimicrobial response. Here, we examine a putative ecdysteroid glycosyltransferase, Hba_07292 (Hb-ugt-1), which was previously identified in the hemolymph-activated transcriptome of the entomopathogenic nematode Heterorhabditis bacteriophora. Injection of recombinant Hb-ugt-1 (rHb-ugt-1) into Drosophila melanogaster flies resulted in diminished upregulation of antimicrobial peptides associated with both the Toll and Immune deficiency pathways. Ecdysone was implicated in this suppression by a reduction in Broad Complex expression and reduced pupation rates in r Hb-ugt-1-injected larvae. In addition to the finding that H. bacteriophora excreted-secreted products contain glycosyltransferase activity, these results demonstrate that Hb-ugt-1 is an immunosuppressive factor and that its activity likely involves the inactivation of ecdysone. Nature Publishing Group UK 2020-07-23 /pmc/articles/PMC7378173/ /pubmed/32704134 http://dx.doi.org/10.1038/s41598-020-69306-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kenney, Eric Yaparla, Amulya Hawdon, John M. O’Halloran, Damien M. Grayfer, Leon Eleftherianos, Ioannis A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling |
title | A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling |
title_full | A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling |
title_fullStr | A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling |
title_full_unstemmed | A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling |
title_short | A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling |
title_sort | putative udp-glycosyltransferase from heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378173/ https://www.ncbi.nlm.nih.gov/pubmed/32704134 http://dx.doi.org/10.1038/s41598-020-69306-2 |
work_keys_str_mv | AT kenneyeric aputativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling AT yaparlaamulya aputativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling AT hawdonjohnm aputativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling AT ohallorandamienm aputativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling AT grayferleon aputativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling AT eleftherianosioannis aputativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling AT kenneyeric putativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling AT yaparlaamulya putativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling AT hawdonjohnm putativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling AT ohallorandamienm putativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling AT grayferleon putativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling AT eleftherianosioannis putativeudpglycosyltransferasefromheterorhabditisbacteriophorasuppressesantimicrobialpeptidegeneexpressionandfactorsrelatedtoecdysonesignaling |