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Plasma extracellular vesicle proteins are associated with stress-induced myocardial ischemia in women presenting with chest pain

Diagnosing stable ischemic heart disease (IHD) is challenging, especially in females. Currently, no blood test is available. Plasma extracellular vesicles (EV) are emerging as potential biomarker source. We therefore aimed to identify stress induced ischemia due to stable IHD with plasma extracellul...

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Autores principales: Dekker, Mirthe, Waissi, Farahnaz, van Bennekom, Joelle, Silvis, Max J. M., Timmerman, Nathalie, Bank, Ingrid E. M., Walter, Joan E., Mueller, Christian, Schoneveld, A. H., Schiffelers, Raymond M., Pasterkamp, Gerard, Grobbee, Diederick E., de Winter, Robbert J., Mosterd, A., de Kleijn, Dominique P. V., Timmers, Leo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378184/
https://www.ncbi.nlm.nih.gov/pubmed/32704130
http://dx.doi.org/10.1038/s41598-020-69297-0
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author Dekker, Mirthe
Waissi, Farahnaz
van Bennekom, Joelle
Silvis, Max J. M.
Timmerman, Nathalie
Bank, Ingrid E. M.
Walter, Joan E.
Mueller, Christian
Schoneveld, A. H.
Schiffelers, Raymond M.
Pasterkamp, Gerard
Grobbee, Diederick E.
de Winter, Robbert J.
Mosterd, A.
de Kleijn, Dominique P. V.
Timmers, Leo
author_facet Dekker, Mirthe
Waissi, Farahnaz
van Bennekom, Joelle
Silvis, Max J. M.
Timmerman, Nathalie
Bank, Ingrid E. M.
Walter, Joan E.
Mueller, Christian
Schoneveld, A. H.
Schiffelers, Raymond M.
Pasterkamp, Gerard
Grobbee, Diederick E.
de Winter, Robbert J.
Mosterd, A.
de Kleijn, Dominique P. V.
Timmers, Leo
author_sort Dekker, Mirthe
collection PubMed
description Diagnosing stable ischemic heart disease (IHD) is challenging, especially in females. Currently, no blood test is available. Plasma extracellular vesicles (EV) are emerging as potential biomarker source. We therefore aimed to identify stress induced ischemia due to stable IHD with plasma extracellular vesicle protein levels in chest pain patients. We analyzed 450 patients suspected for stable IHD who were referred for (82)Rb PET/CT in the outpatient clinic. Blood samples were collected before PET/CT and plasma EVs were isolated in 3 plasma subfractions named: TEX, HDL, LDL. In total 6 proteins were quantified in each of these subfractions using immuno-bead assays. CD14 and CystatinC protein levels were independent significant predictors of stress-induced ischemia in the LDL and the HDL subfraction and SerpinC1 and SerpinG1 protein levels in the HDL fraction. Subgroup-analysis on sex revealed that these associations were completely attributed to the associations in women. None of the significant EV proteins remained significant in men. Plasma EV proteins levels are associated with the presence of stable IHD in females presenting with chest pain. This finding, if confirmed in larger cohort studies could be a crucial step in improving diagnostic assessment of women with suspected IHD.
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spelling pubmed-73781842020-07-24 Plasma extracellular vesicle proteins are associated with stress-induced myocardial ischemia in women presenting with chest pain Dekker, Mirthe Waissi, Farahnaz van Bennekom, Joelle Silvis, Max J. M. Timmerman, Nathalie Bank, Ingrid E. M. Walter, Joan E. Mueller, Christian Schoneveld, A. H. Schiffelers, Raymond M. Pasterkamp, Gerard Grobbee, Diederick E. de Winter, Robbert J. Mosterd, A. de Kleijn, Dominique P. V. Timmers, Leo Sci Rep Article Diagnosing stable ischemic heart disease (IHD) is challenging, especially in females. Currently, no blood test is available. Plasma extracellular vesicles (EV) are emerging as potential biomarker source. We therefore aimed to identify stress induced ischemia due to stable IHD with plasma extracellular vesicle protein levels in chest pain patients. We analyzed 450 patients suspected for stable IHD who were referred for (82)Rb PET/CT in the outpatient clinic. Blood samples were collected before PET/CT and plasma EVs were isolated in 3 plasma subfractions named: TEX, HDL, LDL. In total 6 proteins were quantified in each of these subfractions using immuno-bead assays. CD14 and CystatinC protein levels were independent significant predictors of stress-induced ischemia in the LDL and the HDL subfraction and SerpinC1 and SerpinG1 protein levels in the HDL fraction. Subgroup-analysis on sex revealed that these associations were completely attributed to the associations in women. None of the significant EV proteins remained significant in men. Plasma EV proteins levels are associated with the presence of stable IHD in females presenting with chest pain. This finding, if confirmed in larger cohort studies could be a crucial step in improving diagnostic assessment of women with suspected IHD. Nature Publishing Group UK 2020-07-23 /pmc/articles/PMC7378184/ /pubmed/32704130 http://dx.doi.org/10.1038/s41598-020-69297-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dekker, Mirthe
Waissi, Farahnaz
van Bennekom, Joelle
Silvis, Max J. M.
Timmerman, Nathalie
Bank, Ingrid E. M.
Walter, Joan E.
Mueller, Christian
Schoneveld, A. H.
Schiffelers, Raymond M.
Pasterkamp, Gerard
Grobbee, Diederick E.
de Winter, Robbert J.
Mosterd, A.
de Kleijn, Dominique P. V.
Timmers, Leo
Plasma extracellular vesicle proteins are associated with stress-induced myocardial ischemia in women presenting with chest pain
title Plasma extracellular vesicle proteins are associated with stress-induced myocardial ischemia in women presenting with chest pain
title_full Plasma extracellular vesicle proteins are associated with stress-induced myocardial ischemia in women presenting with chest pain
title_fullStr Plasma extracellular vesicle proteins are associated with stress-induced myocardial ischemia in women presenting with chest pain
title_full_unstemmed Plasma extracellular vesicle proteins are associated with stress-induced myocardial ischemia in women presenting with chest pain
title_short Plasma extracellular vesicle proteins are associated with stress-induced myocardial ischemia in women presenting with chest pain
title_sort plasma extracellular vesicle proteins are associated with stress-induced myocardial ischemia in women presenting with chest pain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378184/
https://www.ncbi.nlm.nih.gov/pubmed/32704130
http://dx.doi.org/10.1038/s41598-020-69297-0
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