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Time-saving method for directly amplifying and capturing a minimal amount of pancreatic tumor-derived mutations from fine-needle aspirates using digital PCR

It is challenging to secure a cytopathologic diagnosis using minute amounts of tumor fluids and tissue fragments. Hence, we developed a rapid, accurate, low-cost method for detecting tumor cell-derived DNA from limited amounts of specimens and samples with a low tumor cellularity, to detect KRAS mut...

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Detalles Bibliográficos
Autores principales:	Ono, Yusuke, Hayashi, Akihiro, Maeda, Chiho, Suzuki, Mayumi, Wada, Reona, Sato, Hiroki, Kawabata, Hidemasa, Okada, Tetsuhiro, Goto, Takuma, Karasaki, Hidenori, Mizukami, Yusuke, Okumura, Toshikatsu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378187/ https://www.ncbi.nlm.nih.gov/pubmed/32704002 http://dx.doi.org/10.1038/s41598-020-69221-6

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