Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels

Allyl- and propargyl ethers of umbelliferone are sensitive probes for palladium and platinum, including anticancer compounds cisplatin, carboplatin and oxaliplatin, and effective for direct visualization of protein and DNA complexes with organometallic compounds in polyacrylamide gels allowing easy...

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Autores principales: Pekarik, Vladimir, Peskova, Marie, Duben, Jakub, Remes, Marek, Heger, Zbynek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378192/
https://www.ncbi.nlm.nih.gov/pubmed/32704011
http://dx.doi.org/10.1038/s41598-020-69336-w
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author Pekarik, Vladimir
Peskova, Marie
Duben, Jakub
Remes, Marek
Heger, Zbynek
author_facet Pekarik, Vladimir
Peskova, Marie
Duben, Jakub
Remes, Marek
Heger, Zbynek
author_sort Pekarik, Vladimir
collection PubMed
description Allyl- and propargyl ethers of umbelliferone are sensitive probes for palladium and platinum, including anticancer compounds cisplatin, carboplatin and oxaliplatin, and effective for direct visualization of protein and DNA complexes with organometallic compounds in polyacrylamide gels allowing easy detection of interactions with analyzed protein or nucleic acid. Both probes can be used for fast evaluation of Pd/Pt binding to nanocarriers relevant in drug targeted therapy or specific clinically relevant target macromolecules.
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spelling pubmed-73781922020-07-24 Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels Pekarik, Vladimir Peskova, Marie Duben, Jakub Remes, Marek Heger, Zbynek Sci Rep Article Allyl- and propargyl ethers of umbelliferone are sensitive probes for palladium and platinum, including anticancer compounds cisplatin, carboplatin and oxaliplatin, and effective for direct visualization of protein and DNA complexes with organometallic compounds in polyacrylamide gels allowing easy detection of interactions with analyzed protein or nucleic acid. Both probes can be used for fast evaluation of Pd/Pt binding to nanocarriers relevant in drug targeted therapy or specific clinically relevant target macromolecules. Nature Publishing Group UK 2020-07-23 /pmc/articles/PMC7378192/ /pubmed/32704011 http://dx.doi.org/10.1038/s41598-020-69336-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pekarik, Vladimir
Peskova, Marie
Duben, Jakub
Remes, Marek
Heger, Zbynek
Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels
title Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels
title_full Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels
title_fullStr Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels
title_full_unstemmed Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels
title_short Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels
title_sort direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378192/
https://www.ncbi.nlm.nih.gov/pubmed/32704011
http://dx.doi.org/10.1038/s41598-020-69336-w
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