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Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells
Exposure to airborne particulate matter (PM) is related to the increased risk of several diseases, including chronic and allergic rhinitis. We have previously shown that atmospheric endotoxin level was positively associated with the number of emergency department visits for asthma even after adjusti...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378315/ https://www.ncbi.nlm.nih.gov/pubmed/32714841 http://dx.doi.org/10.1016/j.toxrep.2020.07.002 |
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author | Yamagishi, Nobuyuki Yamaguchi, Tomoki Kuga, Takahisa Taniguchi, Masanari Khan, Mohammad Shahriar Matsumoto, Takahiro Deguchi, Yuya Nagaoka, Hiroaki Wakabayashi, Keiji Watanabe, Tetsushi |
author_facet | Yamagishi, Nobuyuki Yamaguchi, Tomoki Kuga, Takahisa Taniguchi, Masanari Khan, Mohammad Shahriar Matsumoto, Takahiro Deguchi, Yuya Nagaoka, Hiroaki Wakabayashi, Keiji Watanabe, Tetsushi |
author_sort | Yamagishi, Nobuyuki |
collection | PubMed |
description | Exposure to airborne particulate matter (PM) is related to the increased risk of several diseases, including chronic and allergic rhinitis. We have previously shown that atmospheric endotoxin level was positively associated with the number of emergency department visits for asthma even after adjusting for meteorological factors, suggestive of the significant association between atmospheric endotoxin level and asthma exacerbation. Whether atmospheric endotoxin level is related to inflammatory response induction is, however, unclear. Here, we established stable cell lines to determine the promoter activity of the genes encoding pro-inflammatory cytokines such as tumor necrosis factor alpha, interleukin 6 (IL6), and IL33 by transfection of each reporter plasmid into rat tracheal epithelial EGV-4 T cells. These cells could measure the inflammatory response induced by endotoxin treatment more easily, rapidly, and sensitively than the conventional system using immunodetection assays. Furthermore, we revealed a relationship between atmospheric endotoxin level and inflammatory response induction. Thus, the system established herein may serve as a promising tool to monitor inflammatory response induced upon PM exposure. |
format | Online Article Text |
id | pubmed-7378315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-73783152020-07-24 Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells Yamagishi, Nobuyuki Yamaguchi, Tomoki Kuga, Takahisa Taniguchi, Masanari Khan, Mohammad Shahriar Matsumoto, Takahiro Deguchi, Yuya Nagaoka, Hiroaki Wakabayashi, Keiji Watanabe, Tetsushi Toxicol Rep Regular Article Exposure to airborne particulate matter (PM) is related to the increased risk of several diseases, including chronic and allergic rhinitis. We have previously shown that atmospheric endotoxin level was positively associated with the number of emergency department visits for asthma even after adjusting for meteorological factors, suggestive of the significant association between atmospheric endotoxin level and asthma exacerbation. Whether atmospheric endotoxin level is related to inflammatory response induction is, however, unclear. Here, we established stable cell lines to determine the promoter activity of the genes encoding pro-inflammatory cytokines such as tumor necrosis factor alpha, interleukin 6 (IL6), and IL33 by transfection of each reporter plasmid into rat tracheal epithelial EGV-4 T cells. These cells could measure the inflammatory response induced by endotoxin treatment more easily, rapidly, and sensitively than the conventional system using immunodetection assays. Furthermore, we revealed a relationship between atmospheric endotoxin level and inflammatory response induction. Thus, the system established herein may serve as a promising tool to monitor inflammatory response induced upon PM exposure. Elsevier 2020-07-17 /pmc/articles/PMC7378315/ /pubmed/32714841 http://dx.doi.org/10.1016/j.toxrep.2020.07.002 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Regular Article Yamagishi, Nobuyuki Yamaguchi, Tomoki Kuga, Takahisa Taniguchi, Masanari Khan, Mohammad Shahriar Matsumoto, Takahiro Deguchi, Yuya Nagaoka, Hiroaki Wakabayashi, Keiji Watanabe, Tetsushi Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells |
title | Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells |
title_full | Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells |
title_fullStr | Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells |
title_full_unstemmed | Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells |
title_short | Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells |
title_sort | development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378315/ https://www.ncbi.nlm.nih.gov/pubmed/32714841 http://dx.doi.org/10.1016/j.toxrep.2020.07.002 |
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