Silent hemoglobin variant during capillary electrophoresis: A case report

Hemoglobin (Hb) North Manchester [β51(D2) Pro→His; HBB:c.155 C>A] is a rare Hb β‐globin gene variant that affects glycated Hb measurement values, such as ion‐exchange high‐performance liquid chromatography, in patients with diabetes. This variant was first detected in the UK in 1998. Here, we des...

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Autores principales: Yuan, Yanping, Zhou, Xianghai, Gao, Leili, Ren, Qian, Ji, Linong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378421/
https://www.ncbi.nlm.nih.gov/pubmed/32020779
http://dx.doi.org/10.1111/jdi.13222
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author Yuan, Yanping
Zhou, Xianghai
Gao, Leili
Ren, Qian
Ji, Linong
author_facet Yuan, Yanping
Zhou, Xianghai
Gao, Leili
Ren, Qian
Ji, Linong
author_sort Yuan, Yanping
collection PubMed
description Hemoglobin (Hb) North Manchester [β51(D2) Pro→His; HBB:c.155 C>A] is a rare Hb β‐globin gene variant that affects glycated Hb measurement values, such as ion‐exchange high‐performance liquid chromatography, in patients with diabetes. This variant was first detected in the UK in 1998. Here, we describe the first case involving Hb North Manchester detected incidentally in a patient with type 2 diabetes in Northern China. The Hb variant was discovered by ion‐exchange high‐performance liquid chromatography, yet capillary electrophoresis of both glycated Hb program and Hb program failed to detect it. Subsequently, Sanger sequencing was carried out to help identify the Hb variant.
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spelling pubmed-73784212020-07-27 Silent hemoglobin variant during capillary electrophoresis: A case report Yuan, Yanping Zhou, Xianghai Gao, Leili Ren, Qian Ji, Linong J Diabetes Investig Articles Hemoglobin (Hb) North Manchester [β51(D2) Pro→His; HBB:c.155 C>A] is a rare Hb β‐globin gene variant that affects glycated Hb measurement values, such as ion‐exchange high‐performance liquid chromatography, in patients with diabetes. This variant was first detected in the UK in 1998. Here, we describe the first case involving Hb North Manchester detected incidentally in a patient with type 2 diabetes in Northern China. The Hb variant was discovered by ion‐exchange high‐performance liquid chromatography, yet capillary electrophoresis of both glycated Hb program and Hb program failed to detect it. Subsequently, Sanger sequencing was carried out to help identify the Hb variant. John Wiley and Sons Inc. 2020-03-18 2020-07 /pmc/articles/PMC7378421/ /pubmed/32020779 http://dx.doi.org/10.1111/jdi.13222 Text en © 2020 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Articles
Yuan, Yanping
Zhou, Xianghai
Gao, Leili
Ren, Qian
Ji, Linong
Silent hemoglobin variant during capillary electrophoresis: A case report
title Silent hemoglobin variant during capillary electrophoresis: A case report
title_full Silent hemoglobin variant during capillary electrophoresis: A case report
title_fullStr Silent hemoglobin variant during capillary electrophoresis: A case report
title_full_unstemmed Silent hemoglobin variant during capillary electrophoresis: A case report
title_short Silent hemoglobin variant during capillary electrophoresis: A case report
title_sort silent hemoglobin variant during capillary electrophoresis: a case report
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378421/
https://www.ncbi.nlm.nih.gov/pubmed/32020779
http://dx.doi.org/10.1111/jdi.13222
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AT gaoleili silenthemoglobinvariantduringcapillaryelectrophoresisacasereport
AT renqian silenthemoglobinvariantduringcapillaryelectrophoresisacasereport
AT jilinong silenthemoglobinvariantduringcapillaryelectrophoresisacasereport

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