Cargando…
Celecoxib combined with salirasib strongly inhibits pancreatic cancer cells in 2D and 3D cultures
Background/Aim: Pancreatic adenocarcinoma is a highly malignant tumor. Synergistic combinations of anticancer agents for the effective treatment of pancreatic cancer patients are urgently needed. Here, we investigated the combined effect of celecoxib (CEL) and salirasib (SAL) on pancreatic cancer ce...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378654/ https://www.ncbi.nlm.nih.gov/pubmed/32714082 http://dx.doi.org/10.7150/ijms.47546 |
_version_ | 1783562467882827776 |
---|---|
author | Li, Dongli Ma, Yuran Liu, Wenfeng Ren, Xiang Chen, Min Xu, Xuetao Sheng, Zhaojun Zhang, Kun Zhou, Renping Goodin, Susan Zheng, Xi |
author_facet | Li, Dongli Ma, Yuran Liu, Wenfeng Ren, Xiang Chen, Min Xu, Xuetao Sheng, Zhaojun Zhang, Kun Zhou, Renping Goodin, Susan Zheng, Xi |
author_sort | Li, Dongli |
collection | PubMed |
description | Background/Aim: Pancreatic adenocarcinoma is a highly malignant tumor. Synergistic combinations of anticancer agents for the effective treatment of pancreatic cancer patients are urgently needed. Here, we investigated the combined effect of celecoxib (CEL) and salirasib (SAL) on pancreatic cancer cells. Methods: Cell viability and apoptosis were measured by the trypan blue assay, three-dimensional cultures, propidium iodide staining, and caspase-3 assay. NF-κB activation and the protein levels of Akt, pAkt, and Bcl-2 were determined by the luciferase reporter assay and western blot. Results: Co-treatment with CEL and SAL had stronger effects on decreasing cell viability and inducing apoptosis in Panc-1 cells as compared with each agent individually. This combination strongly inhibited NF-κB activity and reduced pAkt and Bcl-2 levels in Panc-1 cells. Conclusion: SAL in combination with CEL may represent a new approach for effective inhibition of pancreatic cancer. |
format | Online Article Text |
id | pubmed-7378654 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-73786542020-07-24 Celecoxib combined with salirasib strongly inhibits pancreatic cancer cells in 2D and 3D cultures Li, Dongli Ma, Yuran Liu, Wenfeng Ren, Xiang Chen, Min Xu, Xuetao Sheng, Zhaojun Zhang, Kun Zhou, Renping Goodin, Susan Zheng, Xi Int J Med Sci Research Paper Background/Aim: Pancreatic adenocarcinoma is a highly malignant tumor. Synergistic combinations of anticancer agents for the effective treatment of pancreatic cancer patients are urgently needed. Here, we investigated the combined effect of celecoxib (CEL) and salirasib (SAL) on pancreatic cancer cells. Methods: Cell viability and apoptosis were measured by the trypan blue assay, three-dimensional cultures, propidium iodide staining, and caspase-3 assay. NF-κB activation and the protein levels of Akt, pAkt, and Bcl-2 were determined by the luciferase reporter assay and western blot. Results: Co-treatment with CEL and SAL had stronger effects on decreasing cell viability and inducing apoptosis in Panc-1 cells as compared with each agent individually. This combination strongly inhibited NF-κB activity and reduced pAkt and Bcl-2 levels in Panc-1 cells. Conclusion: SAL in combination with CEL may represent a new approach for effective inhibition of pancreatic cancer. Ivyspring International Publisher 2020-07-09 /pmc/articles/PMC7378654/ /pubmed/32714082 http://dx.doi.org/10.7150/ijms.47546 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Li, Dongli Ma, Yuran Liu, Wenfeng Ren, Xiang Chen, Min Xu, Xuetao Sheng, Zhaojun Zhang, Kun Zhou, Renping Goodin, Susan Zheng, Xi Celecoxib combined with salirasib strongly inhibits pancreatic cancer cells in 2D and 3D cultures |
title | Celecoxib combined with salirasib strongly inhibits pancreatic cancer cells in 2D and 3D cultures |
title_full | Celecoxib combined with salirasib strongly inhibits pancreatic cancer cells in 2D and 3D cultures |
title_fullStr | Celecoxib combined with salirasib strongly inhibits pancreatic cancer cells in 2D and 3D cultures |
title_full_unstemmed | Celecoxib combined with salirasib strongly inhibits pancreatic cancer cells in 2D and 3D cultures |
title_short | Celecoxib combined with salirasib strongly inhibits pancreatic cancer cells in 2D and 3D cultures |
title_sort | celecoxib combined with salirasib strongly inhibits pancreatic cancer cells in 2d and 3d cultures |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378654/ https://www.ncbi.nlm.nih.gov/pubmed/32714082 http://dx.doi.org/10.7150/ijms.47546 |
work_keys_str_mv | AT lidongli celecoxibcombinedwithsalirasibstronglyinhibitspancreaticcancercellsin2dand3dcultures AT mayuran celecoxibcombinedwithsalirasibstronglyinhibitspancreaticcancercellsin2dand3dcultures AT liuwenfeng celecoxibcombinedwithsalirasibstronglyinhibitspancreaticcancercellsin2dand3dcultures AT renxiang celecoxibcombinedwithsalirasibstronglyinhibitspancreaticcancercellsin2dand3dcultures AT chenmin celecoxibcombinedwithsalirasibstronglyinhibitspancreaticcancercellsin2dand3dcultures AT xuxuetao celecoxibcombinedwithsalirasibstronglyinhibitspancreaticcancercellsin2dand3dcultures AT shengzhaojun celecoxibcombinedwithsalirasibstronglyinhibitspancreaticcancercellsin2dand3dcultures AT zhangkun celecoxibcombinedwithsalirasibstronglyinhibitspancreaticcancercellsin2dand3dcultures AT zhourenping celecoxibcombinedwithsalirasibstronglyinhibitspancreaticcancercellsin2dand3dcultures AT goodinsusan celecoxibcombinedwithsalirasibstronglyinhibitspancreaticcancercellsin2dand3dcultures AT zhengxi celecoxibcombinedwithsalirasibstronglyinhibitspancreaticcancercellsin2dand3dcultures |