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Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization

Retinal neovascularization (RNV) is characterized in retinopathy of prematurity (ROP), diabetic retinopathy (DR), and retinal vein occlusion (RVO), which leads to severe vision loss and even blindness. To reveal the altered transfer RNA-derived small RNA (tsRNA)s in RNV, and to investigate the under...

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Autores principales: Peng, Yingqian, Zou, Jingling, Wang, Jiang-Hui, Zeng, Huilan, Tan, Wei, Yoshida, Shigeo, Zhang, Liwei, Li, Yun, Zhou, Yedi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378657/
https://www.ncbi.nlm.nih.gov/pubmed/32714074
http://dx.doi.org/10.7150/ijms.46209
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author Peng, Yingqian
Zou, Jingling
Wang, Jiang-Hui
Zeng, Huilan
Tan, Wei
Yoshida, Shigeo
Zhang, Liwei
Li, Yun
Zhou, Yedi
author_facet Peng, Yingqian
Zou, Jingling
Wang, Jiang-Hui
Zeng, Huilan
Tan, Wei
Yoshida, Shigeo
Zhang, Liwei
Li, Yun
Zhou, Yedi
author_sort Peng, Yingqian
collection PubMed
description Retinal neovascularization (RNV) is characterized in retinopathy of prematurity (ROP), diabetic retinopathy (DR), and retinal vein occlusion (RVO), which leads to severe vision loss and even blindness. To reveal the altered transfer RNA-derived small RNA (tsRNA)s in RNV, and to investigate the underlying mechanisms of the altered tsRNAs involved in RNV, we carried out a small RNA sequencing to profile tsRNA expressions in the retinas of mice with oxygen-induced retinopathy (OIR) and control mice. A total of 45 tsRNAs were significantly changed (fold change ≥ 1.5 and P < 0.05) in the retinas of OIR mice compared with controls. Validation by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) in four selected tsRNAs was consistent with the results of small RNA sequencing. Bioinformatics analyses identified 153 altered target genes of the four validated tsRNAs. These altered target genes were largely enriched in developmental process, cell periphery and protein binding, as well as Th1 and Th2 cell differentiation pathway. Our study suggests tsRNAs play key roles in the pathogenesis of RNV, indicating their therapeutic potential to treat patients with RNV. Moreover, small RNA sequencing is a useful tool to identify changes in tsRNA expression, an important indicator of the progress of retinal diseases.
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spelling pubmed-73786572020-07-24 Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization Peng, Yingqian Zou, Jingling Wang, Jiang-Hui Zeng, Huilan Tan, Wei Yoshida, Shigeo Zhang, Liwei Li, Yun Zhou, Yedi Int J Med Sci Research Paper Retinal neovascularization (RNV) is characterized in retinopathy of prematurity (ROP), diabetic retinopathy (DR), and retinal vein occlusion (RVO), which leads to severe vision loss and even blindness. To reveal the altered transfer RNA-derived small RNA (tsRNA)s in RNV, and to investigate the underlying mechanisms of the altered tsRNAs involved in RNV, we carried out a small RNA sequencing to profile tsRNA expressions in the retinas of mice with oxygen-induced retinopathy (OIR) and control mice. A total of 45 tsRNAs were significantly changed (fold change ≥ 1.5 and P < 0.05) in the retinas of OIR mice compared with controls. Validation by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) in four selected tsRNAs was consistent with the results of small RNA sequencing. Bioinformatics analyses identified 153 altered target genes of the four validated tsRNAs. These altered target genes were largely enriched in developmental process, cell periphery and protein binding, as well as Th1 and Th2 cell differentiation pathway. Our study suggests tsRNAs play key roles in the pathogenesis of RNV, indicating their therapeutic potential to treat patients with RNV. Moreover, small RNA sequencing is a useful tool to identify changes in tsRNA expression, an important indicator of the progress of retinal diseases. Ivyspring International Publisher 2020-07-06 /pmc/articles/PMC7378657/ /pubmed/32714074 http://dx.doi.org/10.7150/ijms.46209 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Peng, Yingqian
Zou, Jingling
Wang, Jiang-Hui
Zeng, Huilan
Tan, Wei
Yoshida, Shigeo
Zhang, Liwei
Li, Yun
Zhou, Yedi
Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization
title Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization
title_full Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization
title_fullStr Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization
title_full_unstemmed Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization
title_short Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization
title_sort small rna sequencing reveals transfer rna-derived small rna expression profiles in retinal neovascularization
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378657/
https://www.ncbi.nlm.nih.gov/pubmed/32714074
http://dx.doi.org/10.7150/ijms.46209
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