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Ethylene Enhances Seed Germination and Seedling Growth Under Salinity by Reducing Oxidative Stress and Promoting Chlorophyll Content via ETR2 Pathway

Alfalfa (Medicago sativa L.) is an important forage, and salinity is a major stress factor on its yield. In this study, we show that osmotic stress retards alfalfa seedling growth, while ionic/oxidative stress reduces its seed germination. Ethylene treatment can recover the germination rate of alfal...

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Autores principales: Wang, Yue, Diao, Pengfei, Kong, Lingqi, Yu, Ruonan, Zhang, Man, Zuo, Tiantian, Fan, Yanyan, Niu, Yiding, Yan, Fang, Wuriyanghan, Hada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378865/
https://www.ncbi.nlm.nih.gov/pubmed/32765554
http://dx.doi.org/10.3389/fpls.2020.01066
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author Wang, Yue
Diao, Pengfei
Kong, Lingqi
Yu, Ruonan
Zhang, Man
Zuo, Tiantian
Fan, Yanyan
Niu, Yiding
Yan, Fang
Wuriyanghan, Hada
author_facet Wang, Yue
Diao, Pengfei
Kong, Lingqi
Yu, Ruonan
Zhang, Man
Zuo, Tiantian
Fan, Yanyan
Niu, Yiding
Yan, Fang
Wuriyanghan, Hada
author_sort Wang, Yue
collection PubMed
description Alfalfa (Medicago sativa L.) is an important forage, and salinity is a major stress factor on its yield. In this study, we show that osmotic stress retards alfalfa seedling growth, while ionic/oxidative stress reduces its seed germination. Ethylene treatment can recover the germination rate of alfalfa seeds under salt stress, while ethylene inhibitor silver thiosulfate exacerbates salt effects. ETH reduces the accumulation of MDA and H(2)O(2) and increases POD activity. ETH and ACC improve the salt tolerance of alfalfa by increasing proline content under salt stress. In contrast, STS inhibits alfalfa seed germination by reducing POD activity. NaCl treatment reduces chlorophyll content in alfalfa leaves, while ETH and ACC can increase the chlorophyll content and promote seedling growth. ETH promotes the growth of alfalfa in saline condition by reducing the expression of MsACO and MsERF8 genes, while increases its germination rate by upregulating MsERF11 gene. Silencing of MsETR2, a putative ethylene receptor gene in alfalfa, abolishes ethylene triggered tolerance to salt stress. In summary, we show that ethylene improves salt tolerance in alfalfa via MsETR2 dependent manner, and we also analyze the regulatory mechanism of ethylene during germination of alfalfa seeds under salt stress.
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spelling pubmed-73788652020-08-05 Ethylene Enhances Seed Germination and Seedling Growth Under Salinity by Reducing Oxidative Stress and Promoting Chlorophyll Content via ETR2 Pathway Wang, Yue Diao, Pengfei Kong, Lingqi Yu, Ruonan Zhang, Man Zuo, Tiantian Fan, Yanyan Niu, Yiding Yan, Fang Wuriyanghan, Hada Front Plant Sci Plant Science Alfalfa (Medicago sativa L.) is an important forage, and salinity is a major stress factor on its yield. In this study, we show that osmotic stress retards alfalfa seedling growth, while ionic/oxidative stress reduces its seed germination. Ethylene treatment can recover the germination rate of alfalfa seeds under salt stress, while ethylene inhibitor silver thiosulfate exacerbates salt effects. ETH reduces the accumulation of MDA and H(2)O(2) and increases POD activity. ETH and ACC improve the salt tolerance of alfalfa by increasing proline content under salt stress. In contrast, STS inhibits alfalfa seed germination by reducing POD activity. NaCl treatment reduces chlorophyll content in alfalfa leaves, while ETH and ACC can increase the chlorophyll content and promote seedling growth. ETH promotes the growth of alfalfa in saline condition by reducing the expression of MsACO and MsERF8 genes, while increases its germination rate by upregulating MsERF11 gene. Silencing of MsETR2, a putative ethylene receptor gene in alfalfa, abolishes ethylene triggered tolerance to salt stress. In summary, we show that ethylene improves salt tolerance in alfalfa via MsETR2 dependent manner, and we also analyze the regulatory mechanism of ethylene during germination of alfalfa seeds under salt stress. Frontiers Media S.A. 2020-07-16 /pmc/articles/PMC7378865/ /pubmed/32765554 http://dx.doi.org/10.3389/fpls.2020.01066 Text en Copyright © 2020 Wang, Diao, Kong, Yu, Zhang, Zuo, Fan, Niu, Yan and Wuriyanghan http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Yue
Diao, Pengfei
Kong, Lingqi
Yu, Ruonan
Zhang, Man
Zuo, Tiantian
Fan, Yanyan
Niu, Yiding
Yan, Fang
Wuriyanghan, Hada
Ethylene Enhances Seed Germination and Seedling Growth Under Salinity by Reducing Oxidative Stress and Promoting Chlorophyll Content via ETR2 Pathway
title Ethylene Enhances Seed Germination and Seedling Growth Under Salinity by Reducing Oxidative Stress and Promoting Chlorophyll Content via ETR2 Pathway
title_full Ethylene Enhances Seed Germination and Seedling Growth Under Salinity by Reducing Oxidative Stress and Promoting Chlorophyll Content via ETR2 Pathway
title_fullStr Ethylene Enhances Seed Germination and Seedling Growth Under Salinity by Reducing Oxidative Stress and Promoting Chlorophyll Content via ETR2 Pathway
title_full_unstemmed Ethylene Enhances Seed Germination and Seedling Growth Under Salinity by Reducing Oxidative Stress and Promoting Chlorophyll Content via ETR2 Pathway
title_short Ethylene Enhances Seed Germination and Seedling Growth Under Salinity by Reducing Oxidative Stress and Promoting Chlorophyll Content via ETR2 Pathway
title_sort ethylene enhances seed germination and seedling growth under salinity by reducing oxidative stress and promoting chlorophyll content via etr2 pathway
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378865/
https://www.ncbi.nlm.nih.gov/pubmed/32765554
http://dx.doi.org/10.3389/fpls.2020.01066
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