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Using metabarcoding to compare the suitability of two blood‐feeding leech species for sampling mammalian diversity in North Borneo
The application of high‐throughput sequencing (HTS) for metabarcoding of mixed samples offers new opportunities in conservation biology. Recently, the successful detection of prey DNA from the guts of leeches has raised the possibility that these, and other blood‐feeding invertebrates, might serve a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7379310/ https://www.ncbi.nlm.nih.gov/pubmed/30225935 http://dx.doi.org/10.1111/1755-0998.12943 |
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author | Drinkwater, Rosie Schnell, Ida Bærholm Bohmann, Kristine Bernard, Henry Veron, Géraldine Clare, Elizabeth Gilbert, M. Thomas P. Rossiter, Stephen J. |
author_facet | Drinkwater, Rosie Schnell, Ida Bærholm Bohmann, Kristine Bernard, Henry Veron, Géraldine Clare, Elizabeth Gilbert, M. Thomas P. Rossiter, Stephen J. |
author_sort | Drinkwater, Rosie |
collection | PubMed |
description | The application of high‐throughput sequencing (HTS) for metabarcoding of mixed samples offers new opportunities in conservation biology. Recently, the successful detection of prey DNA from the guts of leeches has raised the possibility that these, and other blood‐feeding invertebrates, might serve as useful samplers of mammals. Yet little is known about whether sympatric leech species differ in their feeding preferences, and whether this has a bearing on their relative suitability for monitoring local mammalian diversity. To address these questions, we collected spatially matched samples of two congeneric leech species Haemadipsa picta and Haemadipsa sumatrana from lowland rainforest in Borneo. For each species, we pooled ~500 leeches into batches of 10 individuals, performed PCR to target a section of the mammalian 16S rRNA locus and undertook sequencing of amplicon libraries using an Illumina MiSeq. In total, we identified sequences from 14 mammalian genera, spanning nine families and five orders. We found greater numbers of detections, and higher diversity of OTUs, in H. picta compared with H. sumatrana, with rodents only present in the former leech species. However, comparison of samples from across the landscape revealed no significant difference in mammal community composition between the leech species. We therefore suggest that H. picta is the more suitable iDNA sampler in this degraded Bornean forest. We conclude that the choice of invertebrate sampler can influence the detectability of different mammal groups and that this should be accounted for when designing iDNA studies. |
format | Online Article Text |
id | pubmed-7379310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73793102020-07-24 Using metabarcoding to compare the suitability of two blood‐feeding leech species for sampling mammalian diversity in North Borneo Drinkwater, Rosie Schnell, Ida Bærholm Bohmann, Kristine Bernard, Henry Veron, Géraldine Clare, Elizabeth Gilbert, M. Thomas P. Rossiter, Stephen J. Mol Ecol Resour RESOURCE ARTICLES The application of high‐throughput sequencing (HTS) for metabarcoding of mixed samples offers new opportunities in conservation biology. Recently, the successful detection of prey DNA from the guts of leeches has raised the possibility that these, and other blood‐feeding invertebrates, might serve as useful samplers of mammals. Yet little is known about whether sympatric leech species differ in their feeding preferences, and whether this has a bearing on their relative suitability for monitoring local mammalian diversity. To address these questions, we collected spatially matched samples of two congeneric leech species Haemadipsa picta and Haemadipsa sumatrana from lowland rainforest in Borneo. For each species, we pooled ~500 leeches into batches of 10 individuals, performed PCR to target a section of the mammalian 16S rRNA locus and undertook sequencing of amplicon libraries using an Illumina MiSeq. In total, we identified sequences from 14 mammalian genera, spanning nine families and five orders. We found greater numbers of detections, and higher diversity of OTUs, in H. picta compared with H. sumatrana, with rodents only present in the former leech species. However, comparison of samples from across the landscape revealed no significant difference in mammal community composition between the leech species. We therefore suggest that H. picta is the more suitable iDNA sampler in this degraded Bornean forest. We conclude that the choice of invertebrate sampler can influence the detectability of different mammal groups and that this should be accounted for when designing iDNA studies. John Wiley and Sons Inc. 2018-10-16 2019-01 /pmc/articles/PMC7379310/ /pubmed/30225935 http://dx.doi.org/10.1111/1755-0998.12943 Text en © 2018 The Authors. Molecular Ecology Resources Published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RESOURCE ARTICLES Drinkwater, Rosie Schnell, Ida Bærholm Bohmann, Kristine Bernard, Henry Veron, Géraldine Clare, Elizabeth Gilbert, M. Thomas P. Rossiter, Stephen J. Using metabarcoding to compare the suitability of two blood‐feeding leech species for sampling mammalian diversity in North Borneo |
title | Using metabarcoding to compare the suitability of two blood‐feeding leech species for sampling mammalian diversity in North Borneo |
title_full | Using metabarcoding to compare the suitability of two blood‐feeding leech species for sampling mammalian diversity in North Borneo |
title_fullStr | Using metabarcoding to compare the suitability of two blood‐feeding leech species for sampling mammalian diversity in North Borneo |
title_full_unstemmed | Using metabarcoding to compare the suitability of two blood‐feeding leech species for sampling mammalian diversity in North Borneo |
title_short | Using metabarcoding to compare the suitability of two blood‐feeding leech species for sampling mammalian diversity in North Borneo |
title_sort | using metabarcoding to compare the suitability of two blood‐feeding leech species for sampling mammalian diversity in north borneo |
topic | RESOURCE ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7379310/ https://www.ncbi.nlm.nih.gov/pubmed/30225935 http://dx.doi.org/10.1111/1755-0998.12943 |
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