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High single‐cell diversity in carbon and nitrogen assimilations by a chain‐forming diatom across a century
Almost a century ago Redfield discovered a relatively constant ratio between carbon, nitrogen and phosphorus in particulate organic matter and nitrogen and phosphorus of dissolved nutrients in seawater. Since then, the riverine export of nitrogen to the ocean has increased 20 fold. High abundance of...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7379523/ https://www.ncbi.nlm.nih.gov/pubmed/30277299 http://dx.doi.org/10.1111/1462-2920.14434 |
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author | Olofsson, Malin Kourtchenko, Olga Zetsche, Eva‐Maria Marchant, Hannah K. Whitehouse, Martin J. Godhe, Anna Ploug, Helle |
author_facet | Olofsson, Malin Kourtchenko, Olga Zetsche, Eva‐Maria Marchant, Hannah K. Whitehouse, Martin J. Godhe, Anna Ploug, Helle |
author_sort | Olofsson, Malin |
collection | PubMed |
description | Almost a century ago Redfield discovered a relatively constant ratio between carbon, nitrogen and phosphorus in particulate organic matter and nitrogen and phosphorus of dissolved nutrients in seawater. Since then, the riverine export of nitrogen to the ocean has increased 20 fold. High abundance of resting stages in sediment layers dated more than a century back indicate that the common planktonic diatom Skeletonema marinoi has endured this eutrophication. We germinated unique genotypes from resting stages originating from isotope‐dated sediment layers (15 and 80 years old) in a eutrophied fjord. Using secondary ion mass spectrometry (SIMS) combined with stable isotopic tracers, we show that the cell‐specific carbon and nitrogen assimilation rates vary by an order of magnitude on a single‐cell level but are significantly correlated during the exponential growth phase, resulting in constant assimilation quota in cells with identical genotypes. The assimilation quota varies largely between different clones independent of age. We hypothesize that the success of S. marinoi in coastal waters may be explained by its high diversity of nutrient demand not only at a clone‐specific level but also at the single‐cell level, whereby the population can sustain and adapt to dynamic nutrient conditions in the environment. |
format | Online Article Text |
id | pubmed-7379523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73795232020-07-24 High single‐cell diversity in carbon and nitrogen assimilations by a chain‐forming diatom across a century Olofsson, Malin Kourtchenko, Olga Zetsche, Eva‐Maria Marchant, Hannah K. Whitehouse, Martin J. Godhe, Anna Ploug, Helle Environ Microbiol Research Articles Almost a century ago Redfield discovered a relatively constant ratio between carbon, nitrogen and phosphorus in particulate organic matter and nitrogen and phosphorus of dissolved nutrients in seawater. Since then, the riverine export of nitrogen to the ocean has increased 20 fold. High abundance of resting stages in sediment layers dated more than a century back indicate that the common planktonic diatom Skeletonema marinoi has endured this eutrophication. We germinated unique genotypes from resting stages originating from isotope‐dated sediment layers (15 and 80 years old) in a eutrophied fjord. Using secondary ion mass spectrometry (SIMS) combined with stable isotopic tracers, we show that the cell‐specific carbon and nitrogen assimilation rates vary by an order of magnitude on a single‐cell level but are significantly correlated during the exponential growth phase, resulting in constant assimilation quota in cells with identical genotypes. The assimilation quota varies largely between different clones independent of age. We hypothesize that the success of S. marinoi in coastal waters may be explained by its high diversity of nutrient demand not only at a clone‐specific level but also at the single‐cell level, whereby the population can sustain and adapt to dynamic nutrient conditions in the environment. John Wiley & Sons, Inc. 2018-10-30 2019-01 /pmc/articles/PMC7379523/ /pubmed/30277299 http://dx.doi.org/10.1111/1462-2920.14434 Text en © 2018 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Olofsson, Malin Kourtchenko, Olga Zetsche, Eva‐Maria Marchant, Hannah K. Whitehouse, Martin J. Godhe, Anna Ploug, Helle High single‐cell diversity in carbon and nitrogen assimilations by a chain‐forming diatom across a century |
title | High single‐cell diversity in carbon and nitrogen assimilations by a chain‐forming diatom across a century |
title_full | High single‐cell diversity in carbon and nitrogen assimilations by a chain‐forming diatom across a century |
title_fullStr | High single‐cell diversity in carbon and nitrogen assimilations by a chain‐forming diatom across a century |
title_full_unstemmed | High single‐cell diversity in carbon and nitrogen assimilations by a chain‐forming diatom across a century |
title_short | High single‐cell diversity in carbon and nitrogen assimilations by a chain‐forming diatom across a century |
title_sort | high single‐cell diversity in carbon and nitrogen assimilations by a chain‐forming diatom across a century |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7379523/ https://www.ncbi.nlm.nih.gov/pubmed/30277299 http://dx.doi.org/10.1111/1462-2920.14434 |
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