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Costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in C57BL/6 mice

BACKGROUND: Costunolide (COS), a naturally occurring sesquiterpene lactone, is known to exert anti‐inflammatory, antioxidant, and anticancer effects. This study was undertaken to investigate the effects of costunolide on the promotion of hair growth. METHODS: Real‐time cell analyzer (RTCA), measurem...

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Autores principales: Kim, Young Eun, Choi, Hyung Chul, Nam, Gaewon, Choi, Bu Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7379667/
https://www.ncbi.nlm.nih.gov/pubmed/29808617
http://dx.doi.org/10.1111/jocd.12674
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author Kim, Young Eun
Choi, Hyung Chul
Nam, Gaewon
Choi, Bu Young
author_facet Kim, Young Eun
Choi, Hyung Chul
Nam, Gaewon
Choi, Bu Young
author_sort Kim, Young Eun
collection PubMed
description BACKGROUND: Costunolide (COS), a naturally occurring sesquiterpene lactone, is known to exert anti‐inflammatory, antioxidant, and anticancer effects. This study was undertaken to investigate the effects of costunolide on the promotion of hair growth. METHODS: Real‐time cell analyzer (RTCA), measurement of 5α‐reductase activity, mRNA expression, and Western blotting were adopted to address whether COS can stimulate the proliferation of human hair follicle dermal papilla cells (hHFDPCs). The effect of COS on in vivo hair growth was examined by reconstitution assay and shaven dorsal skin in C57BL/6 mice. RESULTS: Costunolide significantly promoted the proliferation of hHFDPCs, which is comparable to that of tofacitinib. COS also inhibited the 5α‐reductase activity in hHFDPCs. While COS increased the level of β‐catenin and Gli1 mRNA and proteins, it suppressed transforming growth factor (TGF)‐β1–induced phosphorylation of Smad‐1/5 in hHFDPCs. COS increased the number of cultured hHFDPCs to induce hair follicles from mouse epidermal cells in Spheres formation of reconstitution assay. Topical application of COS on the shaven back of C57BL/6 mice significantly improved the hair growth. CONCLUSIONS: Our results illustrate that COS promotes hair growth in vitro and in vivo by regulating the amount of growth factors and/or the activity of cellular responses through coordination of the WNT‐β‐catenin, hedgehog‐Gli, and TGF‐β1–Smad pathways.
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spelling pubmed-73796672020-07-24 Costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in C57BL/6 mice Kim, Young Eun Choi, Hyung Chul Nam, Gaewon Choi, Bu Young J Cosmet Dermatol Basic Science Cosmetic Dermatology Articles BACKGROUND: Costunolide (COS), a naturally occurring sesquiterpene lactone, is known to exert anti‐inflammatory, antioxidant, and anticancer effects. This study was undertaken to investigate the effects of costunolide on the promotion of hair growth. METHODS: Real‐time cell analyzer (RTCA), measurement of 5α‐reductase activity, mRNA expression, and Western blotting were adopted to address whether COS can stimulate the proliferation of human hair follicle dermal papilla cells (hHFDPCs). The effect of COS on in vivo hair growth was examined by reconstitution assay and shaven dorsal skin in C57BL/6 mice. RESULTS: Costunolide significantly promoted the proliferation of hHFDPCs, which is comparable to that of tofacitinib. COS also inhibited the 5α‐reductase activity in hHFDPCs. While COS increased the level of β‐catenin and Gli1 mRNA and proteins, it suppressed transforming growth factor (TGF)‐β1–induced phosphorylation of Smad‐1/5 in hHFDPCs. COS increased the number of cultured hHFDPCs to induce hair follicles from mouse epidermal cells in Spheres formation of reconstitution assay. Topical application of COS on the shaven back of C57BL/6 mice significantly improved the hair growth. CONCLUSIONS: Our results illustrate that COS promotes hair growth in vitro and in vivo by regulating the amount of growth factors and/or the activity of cellular responses through coordination of the WNT‐β‐catenin, hedgehog‐Gli, and TGF‐β1–Smad pathways. John Wiley and Sons Inc. 2018-05-28 2019-02 /pmc/articles/PMC7379667/ /pubmed/29808617 http://dx.doi.org/10.1111/jocd.12674 Text en © 2018 Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Basic Science Cosmetic Dermatology Articles
Kim, Young Eun
Choi, Hyung Chul
Nam, Gaewon
Choi, Bu Young
Costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in C57BL/6 mice
title Costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in C57BL/6 mice
title_full Costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in C57BL/6 mice
title_fullStr Costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in C57BL/6 mice
title_full_unstemmed Costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in C57BL/6 mice
title_short Costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in C57BL/6 mice
title_sort costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in c57bl/6 mice
topic Basic Science Cosmetic Dermatology Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7379667/
https://www.ncbi.nlm.nih.gov/pubmed/29808617
http://dx.doi.org/10.1111/jocd.12674
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