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mDia1 and Cdc42 Regulate Activin B‐Induced Migration of Bone Marrow‐Derived Mesenchymal Stromal Cells
In a previous study, we have shown that Activin B is a potent chemoattractant for bone marrow‐derived mesenchymal stromal cells (BMSCs). As such, the combination of Activin B and BMSCs significantly accelerated rat skin wound healing. In another study, we showed that RhoA activation plays a key role...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7379979/ https://www.ncbi.nlm.nih.gov/pubmed/30358011 http://dx.doi.org/10.1002/stem.2924 |
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author | Wang, Xueer Tang, Pei Guo, Fukun Zhang, Min Yan, Yuan Huang, Mianbo Chen, Yinghua Zhang, Lu Zhang, Lin |
author_facet | Wang, Xueer Tang, Pei Guo, Fukun Zhang, Min Yan, Yuan Huang, Mianbo Chen, Yinghua Zhang, Lu Zhang, Lin |
author_sort | Wang, Xueer |
collection | PubMed |
description | In a previous study, we have shown that Activin B is a potent chemoattractant for bone marrow‐derived mesenchymal stromal cells (BMSCs). As such, the combination of Activin B and BMSCs significantly accelerated rat skin wound healing. In another study, we showed that RhoA activation plays a key role in Activin B‐induced BMSC migration. However, the role of the immediate downstream effectors of RhoA in this process is unclear. Here, we demonstrated that mammalian homolog of Drosophila diaphanous‐1 (mDia1), a downstream effector of RhoA, exerts a crucial function in Activin B‐induced BMSC migration by promoting membrane ruffling, microtubule morphology, and adhesion signaling dynamics. Furthermore, we showed that Activin B does not change Rac1 activity but increases Cdc42 activity in BMSCs. Inactivation of Cdc42 inhibited Activin B‐stimulated Golgi reorientation and the cell migration of BMSCs. Furthermore, knockdown of mDia1 affected Activin B‐induced BMSC‐mediated wound healing in vivo. In conclusion, this study demonstrated that the RhoA‐mDia1 and Cdc42 pathways regulate Activin B‐induced BMSC migration. This study may help to optimize clinical MSC‐based transplantation strategies to promote skin wound healing. stem cells 2019;37:150–161 |
format | Online Article Text |
id | pubmed-7379979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73799792020-07-27 mDia1 and Cdc42 Regulate Activin B‐Induced Migration of Bone Marrow‐Derived Mesenchymal Stromal Cells Wang, Xueer Tang, Pei Guo, Fukun Zhang, Min Yan, Yuan Huang, Mianbo Chen, Yinghua Zhang, Lu Zhang, Lin Stem Cells Tissue‐Specific Stem Cells In a previous study, we have shown that Activin B is a potent chemoattractant for bone marrow‐derived mesenchymal stromal cells (BMSCs). As such, the combination of Activin B and BMSCs significantly accelerated rat skin wound healing. In another study, we showed that RhoA activation plays a key role in Activin B‐induced BMSC migration. However, the role of the immediate downstream effectors of RhoA in this process is unclear. Here, we demonstrated that mammalian homolog of Drosophila diaphanous‐1 (mDia1), a downstream effector of RhoA, exerts a crucial function in Activin B‐induced BMSC migration by promoting membrane ruffling, microtubule morphology, and adhesion signaling dynamics. Furthermore, we showed that Activin B does not change Rac1 activity but increases Cdc42 activity in BMSCs. Inactivation of Cdc42 inhibited Activin B‐stimulated Golgi reorientation and the cell migration of BMSCs. Furthermore, knockdown of mDia1 affected Activin B‐induced BMSC‐mediated wound healing in vivo. In conclusion, this study demonstrated that the RhoA‐mDia1 and Cdc42 pathways regulate Activin B‐induced BMSC migration. This study may help to optimize clinical MSC‐based transplantation strategies to promote skin wound healing. stem cells 2019;37:150–161 John Wiley & Sons, Inc. 2018-11-09 2019-01 /pmc/articles/PMC7379979/ /pubmed/30358011 http://dx.doi.org/10.1002/stem.2924 Text en © 2018 The Authors stem cells published by Wiley Periodicals, Inc. on behalf of AlphaMed Press This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Tissue‐Specific Stem Cells Wang, Xueer Tang, Pei Guo, Fukun Zhang, Min Yan, Yuan Huang, Mianbo Chen, Yinghua Zhang, Lu Zhang, Lin mDia1 and Cdc42 Regulate Activin B‐Induced Migration of Bone Marrow‐Derived Mesenchymal Stromal Cells |
title | mDia1 and Cdc42 Regulate Activin B‐Induced Migration of Bone Marrow‐Derived Mesenchymal Stromal Cells |
title_full | mDia1 and Cdc42 Regulate Activin B‐Induced Migration of Bone Marrow‐Derived Mesenchymal Stromal Cells |
title_fullStr | mDia1 and Cdc42 Regulate Activin B‐Induced Migration of Bone Marrow‐Derived Mesenchymal Stromal Cells |
title_full_unstemmed | mDia1 and Cdc42 Regulate Activin B‐Induced Migration of Bone Marrow‐Derived Mesenchymal Stromal Cells |
title_short | mDia1 and Cdc42 Regulate Activin B‐Induced Migration of Bone Marrow‐Derived Mesenchymal Stromal Cells |
title_sort | mdia1 and cdc42 regulate activin b‐induced migration of bone marrow‐derived mesenchymal stromal cells |
topic | Tissue‐Specific Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7379979/ https://www.ncbi.nlm.nih.gov/pubmed/30358011 http://dx.doi.org/10.1002/stem.2924 |
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