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CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori
The translationally controlled tumor protein (TCTP) is a highly conserved and multifunctional protein with activities ranging from cytoskeletal regulation to transcription regulation in numerous organisms. In insects, TCTP is essential for cell growth and proliferation. Recently, TCTP has been repor...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380024/ https://www.ncbi.nlm.nih.gov/pubmed/29316276 http://dx.doi.org/10.1111/1744-7917.12567 |
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author | Liu, Zu‐Lian Xu, Jun Ling, Lin Zhang, Ru Shang, Peng Huang, Yong‐Ping |
author_facet | Liu, Zu‐Lian Xu, Jun Ling, Lin Zhang, Ru Shang, Peng Huang, Yong‐Ping |
author_sort | Liu, Zu‐Lian |
collection | PubMed |
description | The translationally controlled tumor protein (TCTP) is a highly conserved and multifunctional protein with activities ranging from cytoskeletal regulation to transcription regulation in numerous organisms. In insects, TCTP is essential for cell growth and proliferation. Recently, TCTP has been reported to affect the innate intestinal immune pathway in the Bombyx mori silkworm, a lepidopteran model insect. However, the comprehensive physiological roles of TCTP in the silkworm remain poorly understood. Here, we performed functional analysis of BmTCTP by using a binary transgenic CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/RNA‐guided CRISPER‐associated protein 9 nucleases) system. Disruption of BmTCTP led to developmental arrestment and subsequent lethality in third instar larvae. Histological analysis revealed that growth impairment originated from decreased cell size, and the proliferation and differentiation of intestinal epithelial cells were also affected. RNA‐seq analysis revealed that genes involved in carbohydrate metabolism, lipid metabolism and digestive system pathways were significantly affected by BmTCTP depletion. Together, the results demonstrated that BmTCTP plays a key role in controlling larval growth and development. |
format | Online Article Text |
id | pubmed-7380024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73800242020-07-27 CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori Liu, Zu‐Lian Xu, Jun Ling, Lin Zhang, Ru Shang, Peng Huang, Yong‐Ping Insect Sci Original Articles The translationally controlled tumor protein (TCTP) is a highly conserved and multifunctional protein with activities ranging from cytoskeletal regulation to transcription regulation in numerous organisms. In insects, TCTP is essential for cell growth and proliferation. Recently, TCTP has been reported to affect the innate intestinal immune pathway in the Bombyx mori silkworm, a lepidopteran model insect. However, the comprehensive physiological roles of TCTP in the silkworm remain poorly understood. Here, we performed functional analysis of BmTCTP by using a binary transgenic CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/RNA‐guided CRISPER‐associated protein 9 nucleases) system. Disruption of BmTCTP led to developmental arrestment and subsequent lethality in third instar larvae. Histological analysis revealed that growth impairment originated from decreased cell size, and the proliferation and differentiation of intestinal epithelial cells were also affected. RNA‐seq analysis revealed that genes involved in carbohydrate metabolism, lipid metabolism and digestive system pathways were significantly affected by BmTCTP depletion. Together, the results demonstrated that BmTCTP plays a key role in controlling larval growth and development. John Wiley and Sons Inc. 2018-02-19 2019-12 /pmc/articles/PMC7380024/ /pubmed/29316276 http://dx.doi.org/10.1111/1744-7917.12567 Text en © 2018 The Authors. Insect Science published by John Wiley & Sons Australia, Ltd on behalf of Institute of Zoology, Chinese Academy of Sciences This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Liu, Zu‐Lian Xu, Jun Ling, Lin Zhang, Ru Shang, Peng Huang, Yong‐Ping CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori |
title | CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori
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title_full | CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori
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title_fullStr | CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori
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title_full_unstemmed | CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori
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title_short | CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori
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title_sort | crispr disruption of tctp gene impaired normal development in the silkworm bombyx mori |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380024/ https://www.ncbi.nlm.nih.gov/pubmed/29316276 http://dx.doi.org/10.1111/1744-7917.12567 |
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