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Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle

The apicomplexan parasite Neospora caninum causes neosporosis, an illness that leads to abortion or stillbirth in cattle, causing massive economic losses to the livestock industry. Rapid and viable diagnosis is the premise of prevention and control for neosporosis. In this study, we screened a new m...

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Autores principales: Wang, Xianmei, Song, Xingju, Yang, Jing, Liu, Qun, Liu, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380076/
https://www.ncbi.nlm.nih.gov/pubmed/32766287
http://dx.doi.org/10.3389/fvets.2020.00357
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author Wang, Xianmei
Song, Xingju
Yang, Jing
Liu, Qun
Liu, Jing
author_facet Wang, Xianmei
Song, Xingju
Yang, Jing
Liu, Qun
Liu, Jing
author_sort Wang, Xianmei
collection PubMed
description The apicomplexan parasite Neospora caninum causes neosporosis, an illness that leads to abortion or stillbirth in cattle, causing massive economic losses to the livestock industry. Rapid and viable diagnosis is the premise of prevention and control for neosporosis. In this study, we screened a new microneme protein 26 (NcMIC26) through western blot and mass spectrometry identification from the excretory secretion antigen (ESA) of N. caninum tachyzoites. NcMIC26 is subcellularly localized to the microneme of parasites. NcMIC26 is a specific antigen of N. caninum and has no cross-reaction with Toxoplasma gondii. Therefore, NcMIC26 has the potential to be a candidate diagnostic antigen for neosporosis. To test this hypothesis, recombinant NcMIC26 (rNcMIC26) was expressed in Escherichia coli (E. coli), and an indirect ELISA for detecting anti-N. caninum antibodies in cattle was established. Compared with that of the indirect immunofluorescent antibody test (IFAT), the positive coincidence rate of the ELISA based on rNcMIC26 was 76.53% (75/98), which was higher than that of an ELISA based on rSRS2 (66.33%), and the negative coincidence rate was 84.62% (33/39). It is noteworthy that 30 positive samples confirmed by IFAT were consistent with the rNcMIC26 ELISA but were negative by the rNcSRS2 ELISA. Our research illustrated that NcMIC26 is a dependable diagnostic marker for the serodiagnosis of N. caninum infection in cattle and could be utilized as a supplementary antigen for missed detection by NcSRS2.
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spelling pubmed-73800762020-08-05 Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle Wang, Xianmei Song, Xingju Yang, Jing Liu, Qun Liu, Jing Front Vet Sci Veterinary Science The apicomplexan parasite Neospora caninum causes neosporosis, an illness that leads to abortion or stillbirth in cattle, causing massive economic losses to the livestock industry. Rapid and viable diagnosis is the premise of prevention and control for neosporosis. In this study, we screened a new microneme protein 26 (NcMIC26) through western blot and mass spectrometry identification from the excretory secretion antigen (ESA) of N. caninum tachyzoites. NcMIC26 is subcellularly localized to the microneme of parasites. NcMIC26 is a specific antigen of N. caninum and has no cross-reaction with Toxoplasma gondii. Therefore, NcMIC26 has the potential to be a candidate diagnostic antigen for neosporosis. To test this hypothesis, recombinant NcMIC26 (rNcMIC26) was expressed in Escherichia coli (E. coli), and an indirect ELISA for detecting anti-N. caninum antibodies in cattle was established. Compared with that of the indirect immunofluorescent antibody test (IFAT), the positive coincidence rate of the ELISA based on rNcMIC26 was 76.53% (75/98), which was higher than that of an ELISA based on rSRS2 (66.33%), and the negative coincidence rate was 84.62% (33/39). It is noteworthy that 30 positive samples confirmed by IFAT were consistent with the rNcMIC26 ELISA but were negative by the rNcSRS2 ELISA. Our research illustrated that NcMIC26 is a dependable diagnostic marker for the serodiagnosis of N. caninum infection in cattle and could be utilized as a supplementary antigen for missed detection by NcSRS2. Frontiers Media S.A. 2020-07-17 /pmc/articles/PMC7380076/ /pubmed/32766287 http://dx.doi.org/10.3389/fvets.2020.00357 Text en Copyright © 2020 Wang, Song, Yang, Liu and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Wang, Xianmei
Song, Xingju
Yang, Jing
Liu, Qun
Liu, Jing
Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle
title Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle
title_full Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle
title_fullStr Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle
title_full_unstemmed Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle
title_short Characterization of Neospora Caninum Microneme Protein 26 and Its Potential Use as a Diagnostic Marker for Neosporosis in Cattle
title_sort characterization of neospora caninum microneme protein 26 and its potential use as a diagnostic marker for neosporosis in cattle
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380076/
https://www.ncbi.nlm.nih.gov/pubmed/32766287
http://dx.doi.org/10.3389/fvets.2020.00357
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