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Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi
Chironomidae is the most ecologically diverse insects in aquatic and semi-aquatic habitats. Propsilocerus akamusi (Tokunaga) is a dominant and ubiquitous chironomid species in Eastern Asia and its morphologically unique larvae are also considered as indicator organisms to detect water contamination,...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380273/ https://www.ncbi.nlm.nih.gov/pubmed/32742817 http://dx.doi.org/10.7717/peerj.9584 |
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author | Yan, Chuncai Sun, Xiaoya Cao, Wei Li, Ruoqun Zhao, Cong Sun, Zeyang Liu, Wenbin Pan, Lina |
author_facet | Yan, Chuncai Sun, Xiaoya Cao, Wei Li, Ruoqun Zhao, Cong Sun, Zeyang Liu, Wenbin Pan, Lina |
author_sort | Yan, Chuncai |
collection | PubMed |
description | Chironomidae is the most ecologically diverse insects in aquatic and semi-aquatic habitats. Propsilocerus akamusi (Tokunaga) is a dominant and ubiquitous chironomid species in Eastern Asia and its morphologically unique larvae are also considered as indicator organisms to detect water contamination, potential toxicity and waterborne pathogens. Since few studies to date have focused on the olfactory system of P. akamusi, our study aims to elucidate the potential functions of chemosensory genes in P. akamusi. In our study, we found that although signals released from male groups might attract female swarmers, it was a completely male-dominated mating process. Sequencing the transcriptome of P. akamusi on an Illumina HiSeq platform generated 4.42, 4.46 and 4.53 Gb of clean reads for heads, legs, and antennae, respectively. 27,609 unigenes, 20,379 coding sequences (CDSs), and 8,073 simple sequence repeats were finally obtained. The gene-level differential expression analysis demonstrated variants among three different tissues, including 2,019 genes specifically expressed in heads, 1,540 genes in legs, and 2,071 genes in antennae. Additionally, we identified an assortment of putative olfactory genes consisting of 34 odorant binding proteins, 17 odorant receptors, 32 gustatory receptors, 22 ionotropic receptors, six chemosensory proteins as well as 3 sensory neuron membrane proteins; their relative abundances in the above three tissues were also determined by RT-qPCR. Our finding could allow a more plausible understanding of certain olfaction-mediated behaviors in groups of this macroinvertebrate. |
format | Online Article Text |
id | pubmed-7380273 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73802732020-07-31 Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi Yan, Chuncai Sun, Xiaoya Cao, Wei Li, Ruoqun Zhao, Cong Sun, Zeyang Liu, Wenbin Pan, Lina PeerJ Entomology Chironomidae is the most ecologically diverse insects in aquatic and semi-aquatic habitats. Propsilocerus akamusi (Tokunaga) is a dominant and ubiquitous chironomid species in Eastern Asia and its morphologically unique larvae are also considered as indicator organisms to detect water contamination, potential toxicity and waterborne pathogens. Since few studies to date have focused on the olfactory system of P. akamusi, our study aims to elucidate the potential functions of chemosensory genes in P. akamusi. In our study, we found that although signals released from male groups might attract female swarmers, it was a completely male-dominated mating process. Sequencing the transcriptome of P. akamusi on an Illumina HiSeq platform generated 4.42, 4.46 and 4.53 Gb of clean reads for heads, legs, and antennae, respectively. 27,609 unigenes, 20,379 coding sequences (CDSs), and 8,073 simple sequence repeats were finally obtained. The gene-level differential expression analysis demonstrated variants among three different tissues, including 2,019 genes specifically expressed in heads, 1,540 genes in legs, and 2,071 genes in antennae. Additionally, we identified an assortment of putative olfactory genes consisting of 34 odorant binding proteins, 17 odorant receptors, 32 gustatory receptors, 22 ionotropic receptors, six chemosensory proteins as well as 3 sensory neuron membrane proteins; their relative abundances in the above three tissues were also determined by RT-qPCR. Our finding could allow a more plausible understanding of certain olfaction-mediated behaviors in groups of this macroinvertebrate. PeerJ Inc. 2020-07-21 /pmc/articles/PMC7380273/ /pubmed/32742817 http://dx.doi.org/10.7717/peerj.9584 Text en ©2020 Yan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Entomology Yan, Chuncai Sun, Xiaoya Cao, Wei Li, Ruoqun Zhao, Cong Sun, Zeyang Liu, Wenbin Pan, Lina Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi |
title | Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi |
title_full | Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi |
title_fullStr | Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi |
title_full_unstemmed | Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi |
title_short | Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi |
title_sort | identification and expression pattern of chemosensory genes in the transcriptome of propsilocerus akamusi |
topic | Entomology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380273/ https://www.ncbi.nlm.nih.gov/pubmed/32742817 http://dx.doi.org/10.7717/peerj.9584 |
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