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Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi

Chironomidae is the most ecologically diverse insects in aquatic and semi-aquatic habitats. Propsilocerus akamusi (Tokunaga) is a dominant and ubiquitous chironomid species in Eastern Asia and its morphologically unique larvae are also considered as indicator organisms to detect water contamination,...

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Autores principales: Yan, Chuncai, Sun, Xiaoya, Cao, Wei, Li, Ruoqun, Zhao, Cong, Sun, Zeyang, Liu, Wenbin, Pan, Lina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380273/
https://www.ncbi.nlm.nih.gov/pubmed/32742817
http://dx.doi.org/10.7717/peerj.9584
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author Yan, Chuncai
Sun, Xiaoya
Cao, Wei
Li, Ruoqun
Zhao, Cong
Sun, Zeyang
Liu, Wenbin
Pan, Lina
author_facet Yan, Chuncai
Sun, Xiaoya
Cao, Wei
Li, Ruoqun
Zhao, Cong
Sun, Zeyang
Liu, Wenbin
Pan, Lina
author_sort Yan, Chuncai
collection PubMed
description Chironomidae is the most ecologically diverse insects in aquatic and semi-aquatic habitats. Propsilocerus akamusi (Tokunaga) is a dominant and ubiquitous chironomid species in Eastern Asia and its morphologically unique larvae are also considered as indicator organisms to detect water contamination, potential toxicity and waterborne pathogens. Since few studies to date have focused on the olfactory system of P. akamusi, our study aims to elucidate the potential functions of chemosensory genes in P. akamusi. In our study, we found that although signals released from male groups might attract female swarmers, it was a completely male-dominated mating process. Sequencing the transcriptome of P. akamusi on an Illumina HiSeq platform generated 4.42, 4.46 and 4.53 Gb of clean reads for heads, legs, and antennae, respectively. 27,609 unigenes, 20,379 coding sequences (CDSs), and 8,073 simple sequence repeats were finally obtained. The gene-level differential expression analysis demonstrated variants among three different tissues, including 2,019 genes specifically expressed in heads, 1,540 genes in legs, and 2,071 genes in antennae. Additionally, we identified an assortment of putative olfactory genes consisting of 34 odorant binding proteins, 17 odorant receptors, 32 gustatory receptors, 22 ionotropic receptors, six chemosensory proteins as well as 3 sensory neuron membrane proteins; their relative abundances in the above three tissues were also determined by RT-qPCR. Our finding could allow a more plausible understanding of certain olfaction-mediated behaviors in groups of this macroinvertebrate.
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spelling pubmed-73802732020-07-31 Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi Yan, Chuncai Sun, Xiaoya Cao, Wei Li, Ruoqun Zhao, Cong Sun, Zeyang Liu, Wenbin Pan, Lina PeerJ Entomology Chironomidae is the most ecologically diverse insects in aquatic and semi-aquatic habitats. Propsilocerus akamusi (Tokunaga) is a dominant and ubiquitous chironomid species in Eastern Asia and its morphologically unique larvae are also considered as indicator organisms to detect water contamination, potential toxicity and waterborne pathogens. Since few studies to date have focused on the olfactory system of P. akamusi, our study aims to elucidate the potential functions of chemosensory genes in P. akamusi. In our study, we found that although signals released from male groups might attract female swarmers, it was a completely male-dominated mating process. Sequencing the transcriptome of P. akamusi on an Illumina HiSeq platform generated 4.42, 4.46 and 4.53 Gb of clean reads for heads, legs, and antennae, respectively. 27,609 unigenes, 20,379 coding sequences (CDSs), and 8,073 simple sequence repeats were finally obtained. The gene-level differential expression analysis demonstrated variants among three different tissues, including 2,019 genes specifically expressed in heads, 1,540 genes in legs, and 2,071 genes in antennae. Additionally, we identified an assortment of putative olfactory genes consisting of 34 odorant binding proteins, 17 odorant receptors, 32 gustatory receptors, 22 ionotropic receptors, six chemosensory proteins as well as 3 sensory neuron membrane proteins; their relative abundances in the above three tissues were also determined by RT-qPCR. Our finding could allow a more plausible understanding of certain olfaction-mediated behaviors in groups of this macroinvertebrate. PeerJ Inc. 2020-07-21 /pmc/articles/PMC7380273/ /pubmed/32742817 http://dx.doi.org/10.7717/peerj.9584 Text en ©2020 Yan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Entomology
Yan, Chuncai
Sun, Xiaoya
Cao, Wei
Li, Ruoqun
Zhao, Cong
Sun, Zeyang
Liu, Wenbin
Pan, Lina
Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi
title Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi
title_full Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi
title_fullStr Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi
title_full_unstemmed Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi
title_short Identification and expression pattern of chemosensory genes in the transcriptome of Propsilocerus akamusi
title_sort identification and expression pattern of chemosensory genes in the transcriptome of propsilocerus akamusi
topic Entomology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380273/
https://www.ncbi.nlm.nih.gov/pubmed/32742817
http://dx.doi.org/10.7717/peerj.9584
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