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Isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish
Coronary heart disease (CHD) is a leading cause of death worldwide, and angiogenesis plays important roles in CHD. Thus, in the present study, the angiogenic efficacy of four common cardiovascular medicines (aspirin, pravastatin, metoprolol and isosorbide mononitrate (ISMN)) was determined by the nu...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380327/ https://www.ncbi.nlm.nih.gov/pubmed/32706844 http://dx.doi.org/10.1590/1678-4685-GMB-2019-0233 |
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author | Lv, Hui Liu, Bo Qin, Yongwen |
author_facet | Lv, Hui Liu, Bo Qin, Yongwen |
author_sort | Lv, Hui |
collection | PubMed |
description | Coronary heart disease (CHD) is a leading cause of death worldwide, and angiogenesis plays important roles in CHD. Thus, in the present study, the angiogenic efficacy of four common cardiovascular medicines (aspirin, pravastatin, metoprolol and isosorbide mononitrate (ISMN)) was determined by the number and length of zebrafish intersegmental vessels (ISVs) after immersing zebrafish embryos in different medicines. Results showed that ISMN significantly increased the length and number of ISVs. ISMN is a long-acting nitrate ester drug. It has been used as a vasodilator to dilate arteries and veins to reduce the cardiac preload and postload. However, the effect of ISMN on angiogenesis remains unclear. Thus, by in vitro experiments, the angiogenic mechanism of ISMN was evaluated through detecting the viability and proliferation of human umbilical vein endothelial cells (HUVECs) and the expression of angiogenesis-related genes and miRNAs. Results indicated that ISMN could increase the viability and proliferation of HUVECs by decreasing apoptosis, and elevated the expressions of vedf, kdrl, pdgfr in zebrafish embryos. Furthermore, the expressions of miR-126, miR-130a and miR-210 were also regulated in ISMN-treated HUVECs. In conclusion, ISMN could promote angiogenesis in zebrafish embryos and HUVECs, implying ISMN may be a potential therapeutic in treating angiogenesis-related diseases. |
format | Online Article Text |
id | pubmed-7380327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-73803272020-08-03 Isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish Lv, Hui Liu, Bo Qin, Yongwen Genet Mol Biol Cellular, Molecular and Developmental Genetics Coronary heart disease (CHD) is a leading cause of death worldwide, and angiogenesis plays important roles in CHD. Thus, in the present study, the angiogenic efficacy of four common cardiovascular medicines (aspirin, pravastatin, metoprolol and isosorbide mononitrate (ISMN)) was determined by the number and length of zebrafish intersegmental vessels (ISVs) after immersing zebrafish embryos in different medicines. Results showed that ISMN significantly increased the length and number of ISVs. ISMN is a long-acting nitrate ester drug. It has been used as a vasodilator to dilate arteries and veins to reduce the cardiac preload and postload. However, the effect of ISMN on angiogenesis remains unclear. Thus, by in vitro experiments, the angiogenic mechanism of ISMN was evaluated through detecting the viability and proliferation of human umbilical vein endothelial cells (HUVECs) and the expression of angiogenesis-related genes and miRNAs. Results indicated that ISMN could increase the viability and proliferation of HUVECs by decreasing apoptosis, and elevated the expressions of vedf, kdrl, pdgfr in zebrafish embryos. Furthermore, the expressions of miR-126, miR-130a and miR-210 were also regulated in ISMN-treated HUVECs. In conclusion, ISMN could promote angiogenesis in zebrafish embryos and HUVECs, implying ISMN may be a potential therapeutic in treating angiogenesis-related diseases. Sociedade Brasileira de Genética 2020-07-24 /pmc/articles/PMC7380327/ /pubmed/32706844 http://dx.doi.org/10.1590/1678-4685-GMB-2019-0233 Text en Copyright © 2020, Sociedade Brasileira de Genética. https://creativecommons.org/licenses/by/4.0/ License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited. |
spellingShingle | Cellular, Molecular and Developmental Genetics Lv, Hui Liu, Bo Qin, Yongwen Isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish |
title | Isosorbide mononitrate promotes angiogenesis in embryonic development
of zebrafish |
title_full | Isosorbide mononitrate promotes angiogenesis in embryonic development
of zebrafish |
title_fullStr | Isosorbide mononitrate promotes angiogenesis in embryonic development
of zebrafish |
title_full_unstemmed | Isosorbide mononitrate promotes angiogenesis in embryonic development
of zebrafish |
title_short | Isosorbide mononitrate promotes angiogenesis in embryonic development
of zebrafish |
title_sort | isosorbide mononitrate promotes angiogenesis in embryonic development
of zebrafish |
topic | Cellular, Molecular and Developmental Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380327/ https://www.ncbi.nlm.nih.gov/pubmed/32706844 http://dx.doi.org/10.1590/1678-4685-GMB-2019-0233 |
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