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Isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish

Coronary heart disease (CHD) is a leading cause of death worldwide, and angiogenesis plays important roles in CHD. Thus, in the present study, the angiogenic efficacy of four common cardiovascular medicines (aspirin, pravastatin, metoprolol and isosorbide mononitrate (ISMN)) was determined by the nu...

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Autores principales: Lv, Hui, Liu, Bo, Qin, Yongwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380327/
https://www.ncbi.nlm.nih.gov/pubmed/32706844
http://dx.doi.org/10.1590/1678-4685-GMB-2019-0233
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author Lv, Hui
Liu, Bo
Qin, Yongwen
author_facet Lv, Hui
Liu, Bo
Qin, Yongwen
author_sort Lv, Hui
collection PubMed
description Coronary heart disease (CHD) is a leading cause of death worldwide, and angiogenesis plays important roles in CHD. Thus, in the present study, the angiogenic efficacy of four common cardiovascular medicines (aspirin, pravastatin, metoprolol and isosorbide mononitrate (ISMN)) was determined by the number and length of zebrafish intersegmental vessels (ISVs) after immersing zebrafish embryos in different medicines. Results showed that ISMN significantly increased the length and number of ISVs. ISMN is a long-acting nitrate ester drug. It has been used as a vasodilator to dilate arteries and veins to reduce the cardiac preload and postload. However, the effect of ISMN on angiogenesis remains unclear. Thus, by in vitro experiments, the angiogenic mechanism of ISMN was evaluated through detecting the viability and proliferation of human umbilical vein endothelial cells (HUVECs) and the expression of angiogenesis-related genes and miRNAs. Results indicated that ISMN could increase the viability and proliferation of HUVECs by decreasing apoptosis, and elevated the expressions of vedf, kdrl, pdgfr in zebrafish embryos. Furthermore, the expressions of miR-126, miR-130a and miR-210 were also regulated in ISMN-treated HUVECs. In conclusion, ISMN could promote angiogenesis in zebrafish embryos and HUVECs, implying ISMN may be a potential therapeutic in treating angiogenesis-related diseases.
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spelling pubmed-73803272020-08-03 Isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish Lv, Hui Liu, Bo Qin, Yongwen Genet Mol Biol Cellular, Molecular and Developmental Genetics Coronary heart disease (CHD) is a leading cause of death worldwide, and angiogenesis plays important roles in CHD. Thus, in the present study, the angiogenic efficacy of four common cardiovascular medicines (aspirin, pravastatin, metoprolol and isosorbide mononitrate (ISMN)) was determined by the number and length of zebrafish intersegmental vessels (ISVs) after immersing zebrafish embryos in different medicines. Results showed that ISMN significantly increased the length and number of ISVs. ISMN is a long-acting nitrate ester drug. It has been used as a vasodilator to dilate arteries and veins to reduce the cardiac preload and postload. However, the effect of ISMN on angiogenesis remains unclear. Thus, by in vitro experiments, the angiogenic mechanism of ISMN was evaluated through detecting the viability and proliferation of human umbilical vein endothelial cells (HUVECs) and the expression of angiogenesis-related genes and miRNAs. Results indicated that ISMN could increase the viability and proliferation of HUVECs by decreasing apoptosis, and elevated the expressions of vedf, kdrl, pdgfr in zebrafish embryos. Furthermore, the expressions of miR-126, miR-130a and miR-210 were also regulated in ISMN-treated HUVECs. In conclusion, ISMN could promote angiogenesis in zebrafish embryos and HUVECs, implying ISMN may be a potential therapeutic in treating angiogenesis-related diseases. Sociedade Brasileira de Genética 2020-07-24 /pmc/articles/PMC7380327/ /pubmed/32706844 http://dx.doi.org/10.1590/1678-4685-GMB-2019-0233 Text en Copyright © 2020, Sociedade Brasileira de Genética. https://creativecommons.org/licenses/by/4.0/ License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited.
spellingShingle Cellular, Molecular and Developmental Genetics
Lv, Hui
Liu, Bo
Qin, Yongwen
Isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish
title Isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish
title_full Isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish
title_fullStr Isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish
title_full_unstemmed Isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish
title_short Isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish
title_sort isosorbide mononitrate promotes angiogenesis in embryonic development of zebrafish
topic Cellular, Molecular and Developmental Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380327/
https://www.ncbi.nlm.nih.gov/pubmed/32706844
http://dx.doi.org/10.1590/1678-4685-GMB-2019-0233
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