Whole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle

Forty-eight Escherichia coli strains were chosen due to variable detection of stx or serogroup by PCR. Although all strains were initially determined to be Shiga toxin-producing Escherichia coli (STEC), their genomes revealed 11 isolates carrying stx(1a), stx(1b), stx(2a), and/or stx(2b). Assembled...

Descripción completa

Detalles Bibliográficos
Autores principales: Castro, Vinicius Silva, de Souza Figueiredo, Eduardo Eustáquio, McAllister, Tim, King, Robin, Reuter, Tim, Ortega Polo, Rodrigo, Conte, Carlos Adam, Stanford, Kim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Genome Sequences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380519/
https://www.ncbi.nlm.nih.gov/pubmed/32273360
http://dx.doi.org/10.1128/MRA.00168-20
_version_ 1783562866630066176
author Castro, Vinicius Silva
de Souza Figueiredo, Eduardo Eustáquio
McAllister, Tim
King, Robin
Reuter, Tim
Ortega Polo, Rodrigo
Conte, Carlos Adam
Stanford, Kim
author_facet Castro, Vinicius Silva
de Souza Figueiredo, Eduardo Eustáquio
McAllister, Tim
King, Robin
Reuter, Tim
Ortega Polo, Rodrigo
Conte, Carlos Adam
Stanford, Kim
author_sort Castro, Vinicius Silva
collection PubMed
description Forty-eight Escherichia coli strains were chosen due to variable detection of stx or serogroup by PCR. Although all strains were initially determined to be Shiga toxin-producing Escherichia coli (STEC), their genomes revealed 11 isolates carrying stx(1a), stx(1b), stx(2a), and/or stx(2b). Assembled genome sizes varied between 4,667,418 and 5,556,121 bp, with N(50) values between 79,648 and 294,166 bp and G+C contents between 50.3% and 51.4%.
format Online
Article
Text
id pubmed-7380519
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-73805192020-07-24 Whole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle Castro, Vinicius Silva de Souza Figueiredo, Eduardo Eustáquio McAllister, Tim King, Robin Reuter, Tim Ortega Polo, Rodrigo Conte, Carlos Adam Stanford, Kim Microbiol Resour Announc Genome Sequences Forty-eight Escherichia coli strains were chosen due to variable detection of stx or serogroup by PCR. Although all strains were initially determined to be Shiga toxin-producing Escherichia coli (STEC), their genomes revealed 11 isolates carrying stx(1a), stx(1b), stx(2a), and/or stx(2b). Assembled genome sizes varied between 4,667,418 and 5,556,121 bp, with N(50) values between 79,648 and 294,166 bp and G+C contents between 50.3% and 51.4%. American Society for Microbiology 2020-04-09 /pmc/articles/PMC7380519/ /pubmed/32273360 http://dx.doi.org/10.1128/MRA.00168-20 Text en Copyright © 2020 Castro et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Genome Sequences
Castro, Vinicius Silva
de Souza Figueiredo, Eduardo Eustáquio
McAllister, Tim
King, Robin
Reuter, Tim
Ortega Polo, Rodrigo
Conte, Carlos Adam
Stanford, Kim
Whole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle
title Whole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle
title_full Whole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle
title_fullStr Whole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle
title_full_unstemmed Whole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle
title_short Whole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle
title_sort whole-genome draft assemblies of difficult-to-classify escherichia coli o157 and non-o157 isolates from feces of canadian feedlot cattle
topic Genome Sequences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380519/
https://www.ncbi.nlm.nih.gov/pubmed/32273360
http://dx.doi.org/10.1128/MRA.00168-20
work_keys_str_mv AT castroviniciussilva wholegenomedraftassembliesofdifficulttoclassifyescherichiacolio157andnono157isolatesfromfecesofcanadianfeedlotcattle
AT desouzafigueiredoeduardoeustaquio wholegenomedraftassembliesofdifficulttoclassifyescherichiacolio157andnono157isolatesfromfecesofcanadianfeedlotcattle
AT mcallistertim wholegenomedraftassembliesofdifficulttoclassifyescherichiacolio157andnono157isolatesfromfecesofcanadianfeedlotcattle
AT kingrobin wholegenomedraftassembliesofdifficulttoclassifyescherichiacolio157andnono157isolatesfromfecesofcanadianfeedlotcattle
AT reutertim wholegenomedraftassembliesofdifficulttoclassifyescherichiacolio157andnono157isolatesfromfecesofcanadianfeedlotcattle
AT ortegapolorodrigo wholegenomedraftassembliesofdifficulttoclassifyescherichiacolio157andnono157isolatesfromfecesofcanadianfeedlotcattle
AT contecarlosadam wholegenomedraftassembliesofdifficulttoclassifyescherichiacolio157andnono157isolatesfromfecesofcanadianfeedlotcattle
AT stanfordkim wholegenomedraftassembliesofdifficulttoclassifyescherichiacolio157andnono157isolatesfromfecesofcanadianfeedlotcattle

Ejemplares similares