Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA

To circumvent the limited availability of RNA extraction reagents, we aimed to develop a protocol for direct RT-qPCR to detect SARS-CoV-2 in nasopharyngeal swabs without RNA extraction. Nasopharyngeal specimens positive for SARS-CoV-2 and other coronaviruses collected in universal viral transport (U...

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Detalles Bibliográficos
Autores principales: Hasan, Mohammad Rubayet, Mirza, Faheem, Al-Hail, Hamad, Sundararaju, Sathyavathi, Xaba, Thabisile, Iqbal, Muhammad, Alhussain, Hashim, Yassine, Hadi Mohamad, Perez-Lopez, Andres, Tang, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380591/
https://www.ncbi.nlm.nih.gov/pubmed/32706827
http://dx.doi.org/10.1371/journal.pone.0236564

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7380591/
https://www.ncbi.nlm.nih.gov/pubmed/32706827
http://dx.doi.org/10.1371/journal.pone.0236564

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