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Intervention of Uncaria and Its Components on Liver Lipid Metabolism in Spontaneously Hypertensive Rats

Uncaria rhynchophylla (Miq.) Miq. ex Havil is widely used in the treatment of hypertension. The Uncaria extract and its bioactives, rhynchophylline and isorhynchophylline, reduced the blood pressure and fatty content in liver cells. In the present study, the antihypertensive effects of Uncaria ethan...

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Autores principales: Tian, Zhenhua, Zhang, Shiming, Wang, Huanjuan, Chen, Zhenshan, Sun, Mengjia, Sun, Linlin, Gong, Lili, Li, Yunlun, Jiang, Haiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381107/
https://www.ncbi.nlm.nih.gov/pubmed/32765256
http://dx.doi.org/10.3389/fphar.2020.00910
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author Tian, Zhenhua
Zhang, Shiming
Wang, Huanjuan
Chen, Zhenshan
Sun, Mengjia
Sun, Linlin
Gong, Lili
Li, Yunlun
Jiang, Haiqiang
author_facet Tian, Zhenhua
Zhang, Shiming
Wang, Huanjuan
Chen, Zhenshan
Sun, Mengjia
Sun, Linlin
Gong, Lili
Li, Yunlun
Jiang, Haiqiang
author_sort Tian, Zhenhua
collection PubMed
description Uncaria rhynchophylla (Miq.) Miq. ex Havil is widely used in the treatment of hypertension. The Uncaria extract and its bioactives, rhynchophylline and isorhynchophylline, reduced the blood pressure and fatty content in liver cells. In the present study, the antihypertensive effects of Uncaria ethanol extract (UET), rhynchophylline (RT) and isorhynchophylline (IT) were investigated in spontaneously hypertensive rats (SHR) using UPLC-Q-Orbitrap/MS based lipidomics approach. Histological changes in the liver were evaluated. Cytolysis and fatty degeneration in the liver tissues were observed in the SHR group. Lipid species in WKY, SHR treated with UET, RT, and IT were plotted to obtain the Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) score plots. Fifty-six endogenous metabolites in the liver such as glycerides, glycerophospholipids, unsaturated fatty acids, and sphingomyelins were selected as potential hypertension associated biomarkers. In order to further explore the metabolite targets of UET for antihypertensive, student's t test and correlation analysis were performed to recognize the pattern recognition and to select the significant metabolites. Similar and prolonged reduction in blood pressure was observed in all SHR groups treated with UET, RT, and IT, while the metabolite profiles were perturbed slightly compared to that of the untreated SHR. Further analysis showed that only a few common components were observed in both RT and IT, which showed similar antihypertensive effect in spite of the distinct metabolic pathways. These results help in understanding the mechanisms of isomeric ingredients in exhibiting the antihypertensive effect but with different targets.
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spelling pubmed-73811072020-08-05 Intervention of Uncaria and Its Components on Liver Lipid Metabolism in Spontaneously Hypertensive Rats Tian, Zhenhua Zhang, Shiming Wang, Huanjuan Chen, Zhenshan Sun, Mengjia Sun, Linlin Gong, Lili Li, Yunlun Jiang, Haiqiang Front Pharmacol Pharmacology Uncaria rhynchophylla (Miq.) Miq. ex Havil is widely used in the treatment of hypertension. The Uncaria extract and its bioactives, rhynchophylline and isorhynchophylline, reduced the blood pressure and fatty content in liver cells. In the present study, the antihypertensive effects of Uncaria ethanol extract (UET), rhynchophylline (RT) and isorhynchophylline (IT) were investigated in spontaneously hypertensive rats (SHR) using UPLC-Q-Orbitrap/MS based lipidomics approach. Histological changes in the liver were evaluated. Cytolysis and fatty degeneration in the liver tissues were observed in the SHR group. Lipid species in WKY, SHR treated with UET, RT, and IT were plotted to obtain the Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) score plots. Fifty-six endogenous metabolites in the liver such as glycerides, glycerophospholipids, unsaturated fatty acids, and sphingomyelins were selected as potential hypertension associated biomarkers. In order to further explore the metabolite targets of UET for antihypertensive, student's t test and correlation analysis were performed to recognize the pattern recognition and to select the significant metabolites. Similar and prolonged reduction in blood pressure was observed in all SHR groups treated with UET, RT, and IT, while the metabolite profiles were perturbed slightly compared to that of the untreated SHR. Further analysis showed that only a few common components were observed in both RT and IT, which showed similar antihypertensive effect in spite of the distinct metabolic pathways. These results help in understanding the mechanisms of isomeric ingredients in exhibiting the antihypertensive effect but with different targets. Frontiers Media S.A. 2020-07-16 /pmc/articles/PMC7381107/ /pubmed/32765256 http://dx.doi.org/10.3389/fphar.2020.00910 Text en Copyright © 2020 Tian, Zhang, Wang, Chen, Sun, Sun, Gong, Li and Jiang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Tian, Zhenhua
Zhang, Shiming
Wang, Huanjuan
Chen, Zhenshan
Sun, Mengjia
Sun, Linlin
Gong, Lili
Li, Yunlun
Jiang, Haiqiang
Intervention of Uncaria and Its Components on Liver Lipid Metabolism in Spontaneously Hypertensive Rats
title Intervention of Uncaria and Its Components on Liver Lipid Metabolism in Spontaneously Hypertensive Rats
title_full Intervention of Uncaria and Its Components on Liver Lipid Metabolism in Spontaneously Hypertensive Rats
title_fullStr Intervention of Uncaria and Its Components on Liver Lipid Metabolism in Spontaneously Hypertensive Rats
title_full_unstemmed Intervention of Uncaria and Its Components on Liver Lipid Metabolism in Spontaneously Hypertensive Rats
title_short Intervention of Uncaria and Its Components on Liver Lipid Metabolism in Spontaneously Hypertensive Rats
title_sort intervention of uncaria and its components on liver lipid metabolism in spontaneously hypertensive rats
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381107/
https://www.ncbi.nlm.nih.gov/pubmed/32765256
http://dx.doi.org/10.3389/fphar.2020.00910
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