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Bisphenol A Diglycidyl Ether (BADGE) and Progesterone Do Not Induce Ca(2+) Signals in Boar Sperm Cells

AIM: Exposure of boar sperm cells to Bisphenol A diglycidyl ether (BADGE) has been shown to lead to reproductive failure in sows, however, the mode of action is unknown. As we have recently shown that BADGE can interfere with Ca(2 +) signaling in human sperm cells through an action on CatSper, and a...

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Autores principales: Rehfeld, Anders, Mendoza, Noelia, Ausejo, Raquel, Skakkebæk, Niels Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381341/
https://www.ncbi.nlm.nih.gov/pubmed/32774306
http://dx.doi.org/10.3389/fphys.2020.00785
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author Rehfeld, Anders
Mendoza, Noelia
Ausejo, Raquel
Skakkebæk, Niels Erik
author_facet Rehfeld, Anders
Mendoza, Noelia
Ausejo, Raquel
Skakkebæk, Niels Erik
author_sort Rehfeld, Anders
collection PubMed
description AIM: Exposure of boar sperm cells to Bisphenol A diglycidyl ether (BADGE) has been shown to lead to reproductive failure in sows, however, the mode of action is unknown. As we have recently shown that BADGE can interfere with Ca(2 +) signaling in human sperm cells through an action on CatSper, and as CatSper has been shown to be expressed in boar sperm cells, we hypothesized that a similar mechanism in the boar sperm cells could be responsible for the reproductive failure. METHODS: Direct effects of BADGE and the endogenous ligand of human CatSper, progesterone, on Ca(2+) signaling in human and boar sperm cells were evaluated side-by-side using a Ca(2+) fluorimetric assay measuring changes in intracellular Ca(2+). Effects of BADGE on Ca(2+) signaling in boar sperm were furthermore assessed by flow cytometry by an independent laboratory. RESULTS: The exact same solutions of BADGE and progesterone induced transient biphasic Ca(2+) signals in human sperm cells, but failed to do so in both non-capacitated and capacitated boar sperm cells. BADGE also failed to induce transient biphasic Ca(2+) signals in boar sperm cells in the flow cytometric assay. CONCLUSION: BADGE and progesterone failed to induce Ca(2+) signals in boar sperm cells. This indicates that the signaling mechanisms leading to activation of CatSper differs between human and boar sperm cells, and suggests that the mode of action by which exposure of boar sperm cells to BADGE can lead to reproductive failure in sows does not involve effects on Ca(2+) signaling.
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spelling pubmed-73813412020-08-06 Bisphenol A Diglycidyl Ether (BADGE) and Progesterone Do Not Induce Ca(2+) Signals in Boar Sperm Cells Rehfeld, Anders Mendoza, Noelia Ausejo, Raquel Skakkebæk, Niels Erik Front Physiol Physiology AIM: Exposure of boar sperm cells to Bisphenol A diglycidyl ether (BADGE) has been shown to lead to reproductive failure in sows, however, the mode of action is unknown. As we have recently shown that BADGE can interfere with Ca(2 +) signaling in human sperm cells through an action on CatSper, and as CatSper has been shown to be expressed in boar sperm cells, we hypothesized that a similar mechanism in the boar sperm cells could be responsible for the reproductive failure. METHODS: Direct effects of BADGE and the endogenous ligand of human CatSper, progesterone, on Ca(2+) signaling in human and boar sperm cells were evaluated side-by-side using a Ca(2+) fluorimetric assay measuring changes in intracellular Ca(2+). Effects of BADGE on Ca(2+) signaling in boar sperm were furthermore assessed by flow cytometry by an independent laboratory. RESULTS: The exact same solutions of BADGE and progesterone induced transient biphasic Ca(2+) signals in human sperm cells, but failed to do so in both non-capacitated and capacitated boar sperm cells. BADGE also failed to induce transient biphasic Ca(2+) signals in boar sperm cells in the flow cytometric assay. CONCLUSION: BADGE and progesterone failed to induce Ca(2+) signals in boar sperm cells. This indicates that the signaling mechanisms leading to activation of CatSper differs between human and boar sperm cells, and suggests that the mode of action by which exposure of boar sperm cells to BADGE can lead to reproductive failure in sows does not involve effects on Ca(2+) signaling. Frontiers Media S.A. 2020-07-07 /pmc/articles/PMC7381341/ /pubmed/32774306 http://dx.doi.org/10.3389/fphys.2020.00785 Text en Copyright © 2020 Rehfeld, Mendoza, Ausejo and Skakkebæk. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Rehfeld, Anders
Mendoza, Noelia
Ausejo, Raquel
Skakkebæk, Niels Erik
Bisphenol A Diglycidyl Ether (BADGE) and Progesterone Do Not Induce Ca(2+) Signals in Boar Sperm Cells
title Bisphenol A Diglycidyl Ether (BADGE) and Progesterone Do Not Induce Ca(2+) Signals in Boar Sperm Cells
title_full Bisphenol A Diglycidyl Ether (BADGE) and Progesterone Do Not Induce Ca(2+) Signals in Boar Sperm Cells
title_fullStr Bisphenol A Diglycidyl Ether (BADGE) and Progesterone Do Not Induce Ca(2+) Signals in Boar Sperm Cells
title_full_unstemmed Bisphenol A Diglycidyl Ether (BADGE) and Progesterone Do Not Induce Ca(2+) Signals in Boar Sperm Cells
title_short Bisphenol A Diglycidyl Ether (BADGE) and Progesterone Do Not Induce Ca(2+) Signals in Boar Sperm Cells
title_sort bisphenol a diglycidyl ether (badge) and progesterone do not induce ca(2+) signals in boar sperm cells
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381341/
https://www.ncbi.nlm.nih.gov/pubmed/32774306
http://dx.doi.org/10.3389/fphys.2020.00785
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