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Ex vivo magnetic particle imaging of vascular inflammation in abdominal aortic aneurysm in a murine model

Abdominal aortic aneurysms (AAAs) are currently one of the leading causes of death in developed countries. Inflammation is crucial in the disease progression, having a substantial impact on various determinants in AAAs development. Magnetic particle imaging (MPI) is an innovative imaging modality, e...

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Autores principales: Mangarova, Dilyana B., Brangsch, Julia, Mohtashamdolatshahi, Azadeh, Kosch, Olaf, Paysen, Hendrik, Wiekhorst, Frank, Klopfleisch, Robert, Buchholz, Rebecca, Karst, Uwe, Taupitz, Matthias, Schnorr, Jörg, Hamm, Bernd, Makowski, Marcus R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381631/
https://www.ncbi.nlm.nih.gov/pubmed/32709967
http://dx.doi.org/10.1038/s41598-020-69299-y
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author Mangarova, Dilyana B.
Brangsch, Julia
Mohtashamdolatshahi, Azadeh
Kosch, Olaf
Paysen, Hendrik
Wiekhorst, Frank
Klopfleisch, Robert
Buchholz, Rebecca
Karst, Uwe
Taupitz, Matthias
Schnorr, Jörg
Hamm, Bernd
Makowski, Marcus R.
author_facet Mangarova, Dilyana B.
Brangsch, Julia
Mohtashamdolatshahi, Azadeh
Kosch, Olaf
Paysen, Hendrik
Wiekhorst, Frank
Klopfleisch, Robert
Buchholz, Rebecca
Karst, Uwe
Taupitz, Matthias
Schnorr, Jörg
Hamm, Bernd
Makowski, Marcus R.
author_sort Mangarova, Dilyana B.
collection PubMed
description Abdominal aortic aneurysms (AAAs) are currently one of the leading causes of death in developed countries. Inflammation is crucial in the disease progression, having a substantial impact on various determinants in AAAs development. Magnetic particle imaging (MPI) is an innovative imaging modality, enabling the highly sensitive detection of magnetic nanoparticles (MNPs), suitable as surrogate marker for molecular targeting of vascular inflammation. For this study, Apolipoprotein E-deficient-mice underwent surgical implantation of osmotic minipumps with constant Angiotensin II infusion. After 3 and 4 weeks respectively, in-vivo-magnetic resonance imaging (MRI), ex-vivo-MPI and ex-vivo-magnetic particle spectroscopy (MPS) were performed. The results were validated by histological analysis, immunohistology and laser ablation-inductively coupled plasma-mass spectrometry. MR-angiography enabled the visualization of aneurysmal development and dilatation in the experimental group. A close correlation (R = 0.87) with histological area assessment was measured. Ex-vivo-MPS revealed abundant iron deposits in AAA samples and ex-vivo histopathology measurements were in good agreement (R = 0.76). Ex-vivo-MPI and MPS results correlated greatly (R = 0.99). CD68-immunohistology stain and Perls’-Prussian-Blue-stain confirmed the colocalization of macrophages and MNPs. This study demonstrates the feasibility of ex-vivo-MPI for detecting inflammation in AAA. The quantitative ability for mapping MNPs establishes MPI as a promising tool for monitoring inflammatory progression in AAA in an experimental setting.
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spelling pubmed-73816312020-07-28 Ex vivo magnetic particle imaging of vascular inflammation in abdominal aortic aneurysm in a murine model Mangarova, Dilyana B. Brangsch, Julia Mohtashamdolatshahi, Azadeh Kosch, Olaf Paysen, Hendrik Wiekhorst, Frank Klopfleisch, Robert Buchholz, Rebecca Karst, Uwe Taupitz, Matthias Schnorr, Jörg Hamm, Bernd Makowski, Marcus R. Sci Rep Article Abdominal aortic aneurysms (AAAs) are currently one of the leading causes of death in developed countries. Inflammation is crucial in the disease progression, having a substantial impact on various determinants in AAAs development. Magnetic particle imaging (MPI) is an innovative imaging modality, enabling the highly sensitive detection of magnetic nanoparticles (MNPs), suitable as surrogate marker for molecular targeting of vascular inflammation. For this study, Apolipoprotein E-deficient-mice underwent surgical implantation of osmotic minipumps with constant Angiotensin II infusion. After 3 and 4 weeks respectively, in-vivo-magnetic resonance imaging (MRI), ex-vivo-MPI and ex-vivo-magnetic particle spectroscopy (MPS) were performed. The results were validated by histological analysis, immunohistology and laser ablation-inductively coupled plasma-mass spectrometry. MR-angiography enabled the visualization of aneurysmal development and dilatation in the experimental group. A close correlation (R = 0.87) with histological area assessment was measured. Ex-vivo-MPS revealed abundant iron deposits in AAA samples and ex-vivo histopathology measurements were in good agreement (R = 0.76). Ex-vivo-MPI and MPS results correlated greatly (R = 0.99). CD68-immunohistology stain and Perls’-Prussian-Blue-stain confirmed the colocalization of macrophages and MNPs. This study demonstrates the feasibility of ex-vivo-MPI for detecting inflammation in AAA. The quantitative ability for mapping MNPs establishes MPI as a promising tool for monitoring inflammatory progression in AAA in an experimental setting. Nature Publishing Group UK 2020-07-24 /pmc/articles/PMC7381631/ /pubmed/32709967 http://dx.doi.org/10.1038/s41598-020-69299-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mangarova, Dilyana B.
Brangsch, Julia
Mohtashamdolatshahi, Azadeh
Kosch, Olaf
Paysen, Hendrik
Wiekhorst, Frank
Klopfleisch, Robert
Buchholz, Rebecca
Karst, Uwe
Taupitz, Matthias
Schnorr, Jörg
Hamm, Bernd
Makowski, Marcus R.
Ex vivo magnetic particle imaging of vascular inflammation in abdominal aortic aneurysm in a murine model
title Ex vivo magnetic particle imaging of vascular inflammation in abdominal aortic aneurysm in a murine model
title_full Ex vivo magnetic particle imaging of vascular inflammation in abdominal aortic aneurysm in a murine model
title_fullStr Ex vivo magnetic particle imaging of vascular inflammation in abdominal aortic aneurysm in a murine model
title_full_unstemmed Ex vivo magnetic particle imaging of vascular inflammation in abdominal aortic aneurysm in a murine model
title_short Ex vivo magnetic particle imaging of vascular inflammation in abdominal aortic aneurysm in a murine model
title_sort ex vivo magnetic particle imaging of vascular inflammation in abdominal aortic aneurysm in a murine model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381631/
https://www.ncbi.nlm.nih.gov/pubmed/32709967
http://dx.doi.org/10.1038/s41598-020-69299-y
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