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A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection

To develop a reproducible and stable closed chest model of ischemic cardiogenic shock in sheep, with high survival rate and potential insight into human pathology. We established a protocol for multi-step myocardial alcoholisation of the left anterior descending coronary artery by percutaneous ethan...

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Autores principales: Rienzo, Mario, Imbault, Julien, El Boustani, Younes, Beurton, Antoine, Carlos Sampedrano, Carolina, Pasdois, Philippe, Pernot, Mathieu, Bernus, Olivier, Haïssaguerre, Michel, Couffinhal, Thierry, Ouattara, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381645/
https://www.ncbi.nlm.nih.gov/pubmed/32709984
http://dx.doi.org/10.1038/s41598-020-68571-5
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author Rienzo, Mario
Imbault, Julien
El Boustani, Younes
Beurton, Antoine
Carlos Sampedrano, Carolina
Pasdois, Philippe
Pernot, Mathieu
Bernus, Olivier
Haïssaguerre, Michel
Couffinhal, Thierry
Ouattara, Alexandre
author_facet Rienzo, Mario
Imbault, Julien
El Boustani, Younes
Beurton, Antoine
Carlos Sampedrano, Carolina
Pasdois, Philippe
Pernot, Mathieu
Bernus, Olivier
Haïssaguerre, Michel
Couffinhal, Thierry
Ouattara, Alexandre
author_sort Rienzo, Mario
collection PubMed
description To develop a reproducible and stable closed chest model of ischemic cardiogenic shock in sheep, with high survival rate and potential insight into human pathology. We established a protocol for multi-step myocardial alcoholisation of the left anterior descending coronary artery by percutaneous ethanol injection. A thorough hemodynamic assessment was obtained by invasive and non-invasive monitoring devices. Repeated blood samples were obtained to determine haemoglobin and alcohol concentration, electrolytes, blood gas parameters and cardiac troponin I. After sacrifice, tissue was excised for quantification of infarction and histology. Cardiogenic shock was characterized by a significant decrease in mean arterial pressure (− 33%), cardiac output (− 29%), dP/dt(max) (− 28%), carotid blood flow (− 22%), left ventricular fractional shortening (− 28%), and left ventricle end-systolic pressure–volume relationship (− 51%). Lactate and cardiac troponin I levels increased from 1.4 ± 0.2 to 4.9 ± 0.7 mmol/L (p = 0.001) and from 0.05 ± 0.02 to 14.74 ± 2.59 µg/L (p = 0.001), respectively. All haemodynamic changes were stable over a three-hour period with a 71% survival rate. The necrotic volume (n = 5) represented 24.0 ± 1.9% of total ventricular mass. No sham exhibited any variation under general anaesthesia. We described and characterized, for the first time, a stable, reproducible sheep model of cardiogenic shock obtained by percutaneous intracoronary ethanol administration.
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spelling pubmed-73816452020-07-28 A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection Rienzo, Mario Imbault, Julien El Boustani, Younes Beurton, Antoine Carlos Sampedrano, Carolina Pasdois, Philippe Pernot, Mathieu Bernus, Olivier Haïssaguerre, Michel Couffinhal, Thierry Ouattara, Alexandre Sci Rep Article To develop a reproducible and stable closed chest model of ischemic cardiogenic shock in sheep, with high survival rate and potential insight into human pathology. We established a protocol for multi-step myocardial alcoholisation of the left anterior descending coronary artery by percutaneous ethanol injection. A thorough hemodynamic assessment was obtained by invasive and non-invasive monitoring devices. Repeated blood samples were obtained to determine haemoglobin and alcohol concentration, electrolytes, blood gas parameters and cardiac troponin I. After sacrifice, tissue was excised for quantification of infarction and histology. Cardiogenic shock was characterized by a significant decrease in mean arterial pressure (− 33%), cardiac output (− 29%), dP/dt(max) (− 28%), carotid blood flow (− 22%), left ventricular fractional shortening (− 28%), and left ventricle end-systolic pressure–volume relationship (− 51%). Lactate and cardiac troponin I levels increased from 1.4 ± 0.2 to 4.9 ± 0.7 mmol/L (p = 0.001) and from 0.05 ± 0.02 to 14.74 ± 2.59 µg/L (p = 0.001), respectively. All haemodynamic changes were stable over a three-hour period with a 71% survival rate. The necrotic volume (n = 5) represented 24.0 ± 1.9% of total ventricular mass. No sham exhibited any variation under general anaesthesia. We described and characterized, for the first time, a stable, reproducible sheep model of cardiogenic shock obtained by percutaneous intracoronary ethanol administration. Nature Publishing Group UK 2020-07-24 /pmc/articles/PMC7381645/ /pubmed/32709984 http://dx.doi.org/10.1038/s41598-020-68571-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Rienzo, Mario
Imbault, Julien
El Boustani, Younes
Beurton, Antoine
Carlos Sampedrano, Carolina
Pasdois, Philippe
Pernot, Mathieu
Bernus, Olivier
Haïssaguerre, Michel
Couffinhal, Thierry
Ouattara, Alexandre
A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection
title A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection
title_full A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection
title_fullStr A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection
title_full_unstemmed A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection
title_short A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection
title_sort total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381645/
https://www.ncbi.nlm.nih.gov/pubmed/32709984
http://dx.doi.org/10.1038/s41598-020-68571-5
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